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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: Functional characterization of fungal lytic polysaccharide monooxygenases for cellulose surface oxidation

Fig. 5

Assessing LPMO (5 µM) activity on SA-Avicel (1%) fiber after 16 h with 1 mM ascorbic acid as an electron donor. T. reesei cellulase cocktail was used to convert all oxidized insoluble products into cellobionic acid and quantify the total C1-oxidized ends introduced to the fiber (nanomoles per mg of starting fiber). Total oxidized ends were obtained by quantifying cellobionic acid by HPAEC-PAD against a standard curve. Each bar is the average of three independent assays measured singly by HPAEC-PAD with error bars indicating the standard error of the mean

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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