Fig. 6

Brightfield and confocal images of LPMO-treated SA-Avicel labeled using cyanine aminooxy dye (blue) for C4 products and rhodamine chloride (green) for C1 products. For each panel, 5 µM LPMOs were incubated at 50 °C for 24 h with 1% SA-Avicel and 1 mM of gallic acid. Insoluble products were separated, labeled with fluorescent dye, and visualized using a confocal microscope. Untreated substrates did not show fluorescence signals (Additional file 1: Figure S2)