Fig. 3
From: Engineering a marine microalga Chlorella sp. as the cell factory
Design and expression foreign genes using native cis elements. a Map of the vector pMEM-CP1. Primers used for PCR amplification of the eGFP fragment and the eBle-mCherry fragment are shown. b PCR amplification of the fragment harboring eGFP in the genome of transformants. Arrow indicates the PCR product with expected size of 1499 bp. c PCR amplification of the eBle-mCherry fragment in the genome of transformants. Arrow indicates the PCR product with expected size of 1305 bp. PC positive control, NC negative control, T1-T2 transformants