Fig. 4

Co-expression of multiple genes in the Chlorella sp. MEM25. a Live-cell fluorescence microscopy of the GFP/BLE-expressing strains. Live cells were plated on agar pads and subjected to fluorescence microscopy. Images were collected using the optimal filters for each fluorescent protein (GFP: excitation 488 nm, emission 520 nm; mCherry: excitation 587 nm, emission 610 nm). For each fluorescent image shown, wild-type images were acquired using identical acquisition settings. Visible light microscope images are also shown. The fluorescent protein and respective WT images were processed identically. Scale bar = 10 µm. b Comparison of GFP or mCherry fluorescence between wild-type and transformant cells. Data are represented as mean ± SD (n = 3 biologically independent samples). *Significant change (**, P ≤ 0.05; ***, P ≤ 0.001. one-sided Student’s t test) versus the WT