Fig. 2

Long-term milking experiments of engineered Synechocystis sp. PCC 6803 strains HX29 and HX42. A Schematic diagram of plasmids used for generating strain HX29. kivd^S286T: encodes α-ketoisovalerate decarboxylase (Lactococcus lactis). B Growth profile, isobutanol (IB) and 3-methyl-1-butanol (3M1B) in-flask and cumulative titers of strain HX29. C Schematic diagram of plasmids used for generating strain HX42. kivd^S286T, encodes α-ketoisovalerate decarboxylase (L. lactis); alsS, encodes acetolactate synthase (Bacillus subtilis); ilvC, encodes acetohydroxy-acid isomeroreductase (Escherichia coli); ilvD, encodes dihydroxy-acid dehydratase (E. coli); slr1192^OP, encodes codon-optimized alcohol dehydrogenase (Synechocystis). D Growth profile, IB and 3M1B in-flask and cumulative titers of strain HX42. Results are the mean of four biological replicates, each with three technical replicates. Error bars represent standard deviation