Fig. 6
Positive effects of rewiring central carbon metabolism on photosynthetic isobutanol (IB) and 3-methyl-1-butanol (3M1B) production. A Western-immunoblot analysis of all overexpressed enzymes. L, ladder (in kDa). Ten micrograms, 20 μg, and 20 μg of total soluble proteins were loaded for each strain to detect Strep-tagged, Flag-tagged, and His-tagged proteins, respectively. Protein size: KivdS286T, 61 kDa; PK, 53 kDa; PCK, 60 kDa; TPI, 27 kDa; AlsS, 62 kDa; FBA, 39 kDa; IlvD, 65 kDa; IlvC, 54 kDa; Slr1192OP, 36 kDa; TK, 72 kDa. B Growth profile of engineered Synechocystis sp. PCC 6803 strains HX74, HX75 and HX77. C Relative IB and 3M1B titers of the engineered Synechocystis strains HX74, HX75 and HX77 on day 10. D Relative IB and 3M1B production per cell of the engineered Synechocystis strains HX74, HX75 and HX77 on day 10. E Relative IB and 3M1B titers of the engineered Synechocystis strains HX81 and HX87 on day 10. F Relative IB and 3M1B production per cell of the engineered Synechocystis strains HX81and HX87 on day 10. G Growth profile of engineered Synechocystis strains HX79 and HX86. H Relative IB and 3M1B titers of the engineered Synechocystis strains HX79 and HX86 on day 10. I Relative IB and 3M1B production per cell of the engineered Synechocystis strains HX79 and HX86 on day 10. J Growth profile of engineered Synechocystis strains HX81 and HX87. Results are the mean of three biological replicates, each with three technical replicates. Error bars represent standard deviation. Asterisk represents significant difference between different strains (one-way ANOVA, * p < 0.05, ** p < 0.005)