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Table 1 List of Synechocystis sp. PCC 6803 strains used in this study

From: Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803

Strain

Relevant genotypesa

References

WT

Wild-type Synechocystis sp. PCC 6803

[12]

HX29

Δddh::(PtrcBCD-kivdS286T-T)-CmR, Δsll1564::(PtrcBCD-kivdS286T-T)-SpR,

pEEK2-(PtrcBCD-kivdS286T-T)-KmR

[15]

HX42

Δddh::(PtrcBCD-slr1192OP-alsS-T)-CmR, Δslr0168::(PtrcBCD-ilvC-ilvD-T)-SpR,

pEEK2-(PtrcBCD-kivdS286T-T)-KmR

[15]

HX56

Δddh::(PtrcBCD-kivdS286T-T)-CmR, Δsll1564::(PtrcBCD-kivdS286T-T)-SpR,

Δslr0168::(PtrcBCD-kivdS286T-T)-EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX61

Δddh::(PtrcBCD-kivdS286T-T)-CmR, Δsll1564::(PtrcBCD-kivdS286T-T)-SpR,

Δslr0168::EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX62

Δddh::(PtrcBCD-kivdS286T-T)-CmR, ΔPEPc::(PtrcBCD-kivdS286T-T)-SpR,

Δslr0168::(PtrcBCD-kivdS286T-T)-EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX63

Δddh::(PtrcBCD-kivdS286T-T)-CmR, Δslr0186::(PtrcBCD-kivdS286T-T)-SpR,

Δslr0168::(PtrcBCD-kivdS286T-T)-EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX74

Δddh::(PtrcBCD-slr1192OP-alsS-T)-CmR, Δslr0168::(PtrcBCD-ilvC-ilvD-T)-SpR,

ΔNSII::(PtrcBCD-fbaA-tktA-T)-EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX75

Δddh::(PtrcBCD-slr1192OP-alsS-T)-CmR, Δslr0168::(PtrcBCD-ilvC-ilvD-T)-SpR,

ΔNSII::EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX77

Δddh::(PtrcBCD-slr1192OP-alsS-T)-CmR, Δslr0168::(PtrcBCD-ilvC-ilvD-T)-SpR,

ΔNSII::(PtrcBCD-pckA-tpiA-T)-EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX78

Δddh::(PtrcBCD-kivdS286T-T)-CmR, Δslr1934::(PtrcBCD-kivdS286T-T)-SpR,

Δslr0168::(PtrcBCD-kivdS286T-T)-EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX79

Δddh::(PtrcBCD-kivdS286T-T)-CmR, Δslr0168::(PtrcBCD-kivdS286T-T)-SpR,

ΔNSII::EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX80

Δddh::(PtrcBCD-kivdS286T-T)-CmR, Δsll1721::(PtrcBCD-kivdS286T-T)-SpR,

Δslr0168::(PtrcBCD-kivdS286T-T)-EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX81

Δddh::(PtrcBCD-kivdS286T-T)-CmR, Δslr0168::(PtrcBCD-kivdS286T-T)-SpR,

ΔNSII::EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX86

Δddh::(PtrcBCD-kivdS286T-T)-CmR, Δslr0168::(PtrcBCD-kivdS286T-T)-SpR,

ΔNSII::(PtrcBCD-pyk1-pckA-T)-EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX87

Δddh::(PtrcBCD-kivdS286T-T)-CmR, Δslr0168::(PtrcBCD-kivdS286T-T)-SpR,

ΔNSII::(PtrcBCD-pckA-tpiA-T)-EmR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX88

Δddh::(PtrcBCD-slr1192OP-sll0065-T)-CmR,

Δslr0168::(PpsbA2-sll1363-slr0452-T)-SpR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX89

Δddh::(PtrcBCD-slr1192OP-sll0065-T)-CmR,

Δslr0168::(PtrcBCD-sll1363-slr0452-T)-SpR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

HX91

Δddh::(PtrcBCD-slr1192OP-slr2088-sll0065-T)-CmR,

Δslr0168::(PtrcBCD-sll1363-slr0452-T)-SpR, pEEK2-(PtrcBCD-kivdS286T-T)-KmR

This study

  1. Expressed genes in bold
  2. aKmR, kanamycin resistance cassette; SpR, spectinomycin resistance cassette; CmR, chloramphenicol resistance cassette; EmR, erythromycin resistance cassette; T, terminator BBa_B0015