Table 2 Isobutanol (IB) and 3-methyl-1-butanol (3M1B) production rates of engineered Synechocystis sp. PCC 6803 strains HX29 and HX42
HX29 | HX42 | |||||||
|---|---|---|---|---|---|---|---|---|
Growth stage | IB (mg L−1 day−1) | 3M1B (mg L−1 day−1) | IB (mg L−1 day−1) | 3M1B (mg L−1 day−1) | ||||
In-flask | Cumulative | In-flask | Cumulative | In-flask | Cumulative | In-flask | Cumulative | |
Stage I (day 0–10) | 25.1 ± 0.6 | 28.0 ± 0.6 | 6.4 ± 0.1 | 7.2 ± 0.1 | 24.1 ± 1.0 | 27.1 ± 1.0 | 6.8 ± 0.1 | 7.7 ± 0.1 |
Stage II (day 10–20) | 16.6 ± 0.8 | 29.8 ± 1.0 | 3.9 ± 0.2 | 7.3 ± 0.2 | 14.0 ± 2.2 | 25.6 ± 2.6 | 5.8 ± 0.6 | 9.5 ± 0.5 |
Stage III (day 20–30) | 3.1 ± 0.3 | 20.5 ± 0.4 | 0.8 ± 0.1 | 5.1 ± 0.1 | 3.2 ± 2.4 | 18.9 ± 4.2 | 1.1 ± 1.3 | 6.4 ± 1.8 |
Stage IV (day 30–40) | 1.5 ± 0.7 | 20.3 ± 0.8 | 0.9 ± 0.2 | 5.6 ± 0.2 | 3.5 ± 1.3 | 21.3 ± 3.3 | 1.4 ± 0.4 | 7.3 ± 1.1 |
Stage V (day 40–50) | 2.4 ± 2.4 | 21.2 ± 2.9 | 0.9 ± 0.6 | 5.9 ± 0.8 | 1.4 ± 6.3 | 20.8 ± 10.1 | 0.4 ± 2.0 | 6.8 ± 3.2 |
Stage VI (day 50–60) | − 12.8 ± 0.6 | 4.2 ± 0.9 | 3.2 ± 0.1 | 1.3 ± 0.3 | − 18.7 ± 5.9 | − 3.3 ± 1.7 | − 6.3 ± 1.9 | − 1.1 ± 0.5 |