Fig. 1

Purification of MrMnP. a FPLC elution profile of the purification step of MrMnP performed on a HiTrapTM Phenyl HP FPLC column. Heme absorption at 407 nm (blue line), total protein at 280 nm (red line), (NH₄)₂SO₄ concentration (brown line), percentage of 10 mM McIlvaine buffer (green line). b UV–Vis absorption spectra of MrMnP. The inset shows SDS-PAGE analysis of purified MrMnP. Lane M: protein ladder; lane 1: crude extract showing expressed MrMnP; lane 2: purified MrMnP (~ 45 kDa)