Fig. 3

Activity assay of PTA and PTB (A) and AcK and BK (B) from strain AK17 (wild-type = WT) and the two other mutants AK17_M3 and AK17_M3ad. A Acetyl-CoA (dark gray) and Butanoyl-CoA (light gray). B Aceyl phosphate (dark gray) and Butanoyl phosphate (light gray). The absence of a bar indicates the product was below the detection limit Data represent the average of three replicate experiments. Standard deviations are shown as error bars