Table 1 List of strains and plasmids used and developed in this study
Plasmid/strain ID | Description | Source |
|---|---|---|
pUC_18 | Cloning vector | Thermo Fisher |
pUC_1801 | Vector for insertion of erm into ldh locus | This study |
pUC_1802 | Vector for insertion of kan into ack/pta loci | This study |
pUC_1803 | Vector for removal of pyrF | This study |
pUC_1804 | Vector for insertion of pyrF into ldh locus | This study |
pUC_1805 | Vector for the removal of pyrF from ldh locus | This study |
pUC_1806 | Vector for insertion of pyrF into bk/ptb loci | This study |
NEB10 | E.coli cloning strain | New England Biolab |
AK17_WT | Thermoanaerobacterium AK17 Wild type | Matís strain collection |
AK17_M1 | AK17_WT1 + pUC1801: Δldh:erm | This study |
AK17_M2 | AK17_WT1 + pUC1802: Δack/pta:kan | This study |
AK17_M3 | AK17_M1 + pUC1802: Δack/pta:kan Δldh:erm | This study |
AK17_M3ad | AK17_M3 after several (> 10) passages | This study |
AK17_M4 | AK17_M3 + pUC1803: Δack/pta:kan Δldh:erm ΔpyrF | This study |
AK17_M5 | AK17_M4 + pUC1804: Δack/pta:kan Δldh:pyrF | This study |
AK17_M6 | AK17_M5 + pUC1805: Δack/pta:kan Δldh ΔpyrF | This study |
AK17_M7 | AK17_M6 + pUC1806: Δack/pta:kan Δldh Δbk/ptb:pyrF | This study |