Table 1 Strains and plasmids used in this study
Strain or plasmid | Description | References or source |
|---|---|---|
Strains | ||
Escherichia coli DH5α | Strain used for plasmid construction | Invitrogen |
Aurantiochytrium sp. SD116 | Wild type | [27] |
ΔPDAT | Disruption of two AuPDAT in SD116 | This study |
ΔPDAT1 | Disruption of one AuPDAT in SD116 | This study |
SD116::AuPDAT | Overexpression of AuPDAT gene in SD116 | This study |
Saccharomyces cerevisiae | ||
H1246 | The TAG-deficient quadruple of S. cerevisiae | [29] |
H1246-pYES2 | H1246 harboring the empty plasmid pYES2 | [29] |
H1246-pDGA1 | H1246 expressing yeast DGA1 gene | [29] |
H1246-pPDAT | H1246 expressing AuPDAT gene | This study |
Plasmids | ||
pWKZ | Plasmid carrying the zeoR gene expression cassette | This study |
pMDZ-GFP | Plasmid carrying the GFP and zeoR gene co-expression cassette and 18 s homologous arm H1 and H2 | [30] |
pMDZ-GFP-PDAT | Plasmid carrying the GFP and AuPDAT gene co-expression cassette | This study |
pWKZ-△PDAT | Plasmid carrying the zeoR gene expression cassette and AuPDAT homologous arm H1 and H2 | This study |
pWKN-△PDAT | Plasmid carrying the neoR gene expression cassette and AuPDAT homologous arm H1 and H2 | This study |
pYES2-PDAT | Plasmid carrying the URA3 gene expression cassette and the AuPDAT gene expression cassette | This study |