Fig. 4

Proof of concept cultivation of U. cynodontis Δfuz7^r Δcyp3^r p_etefmtta p_ria1ria1 in a 2 L fermenter with 100 g/L initial glucose, a linear glucose feed rate and in situ separation of itaconic acid by adsorption. Depicted are oxygen transfer rate (OTR) and concentrations of glucose, itaconic acid and ethanol over time. Cultivation was performed at T = 30 ℃, n = 300–650 rpm, q_in = 60 SL/L/h, pH_initial = 5.78, pH_control = 3.6 (10 M NaOH), OD_600,initial = 0.5 and V_L,initial = 1250 mL in adapted Verduyn medium in a 2-L fermenter. DOT was controlled at 30% by adjusting stirrer speed. Glucose was fed from a 440.7 g/L solution. Column was filled with 11.3 mL of Blücher 100562 activated carbon. The adsorption steps of product separation cycles were performed from 92 to 96 h and 116–118 h. For concentrations, mean values of two replicates are shown