Fig. 2From: In silico design of multipoint mutants for enhanced performance of Thermomyces lanuginosus lipase for efficient biodiesel productionDetermination of enzymatic activity using a pNPP and b olive oil as substrates at 45 °C and pH 8.0 in Tris–HCl buffer. The error bars in graphs represent standard deviations (n = 3). The symbols indicate significant differences between mutants and wild-type: ns (no significant difference), p > 0.05; *, p < 0.05; **, p < 0.01Back to article page