Fig. 3

Saccharification of Avicel with LPMO-containing cellulase cocktails at high dry matter. In the reactions, 25% (w/w) Avicel was incubated with either 3.2 mg/g Celluclast 1.5 L + 0.4 mg/g NZ-BG + 0.4 mg/g LPMO (TaAA9A and TtAA9E in varying ratios), or 3.6 mg/g Celluclast 1.5 L + 0.4 mg/g NZ-BG, or 4 mg/g Cellic CTec2. All reactions contained 10 mM AscA as reductant. Panel A shows the glucose release; panel B shows the total release of oxidized sugars, which is the sum of C4-oxidized products generated by TaAA9A (C) and C1-oxidized products generated by TtAA9E (D). The symbols * and *** in panel A indicate significant differences (p ≤ 0.05 and p ≤ 0.01, respectively) between the cellulase cocktail spiked with TtAA9E only (0:100) and the other enzyme combinations after 72 h (by Student's t-test). Soluble oxidized products were not detected in the reactions without LPMO. Standard deviations are shown as error bars, for three biological replicates