Fig. 4

Initiating LPMO activity by adding ascorbic acid at different time points. In the reactions, 25% (w/w) Avicel was incubated with 3.2 mg/g Celluclast 1.5 L + 0.4 mg/g NZ-BG + 0.4 mg/g of either TaAA9A or TtAA9E. The LPMO activity was initiated by adding AscA (10 mM) at different time points. If added, EDTA was present at 6.3 mM. Panel A shows glucose release; panel B shows the release of soluble oxidized sugars. Standard deviations are shown as error bars, for three biological replicates