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Table 2 Data from batch fermentation tests in shake flasks with different initial concentrations of glucose and AA

From: Development of a hybrid bio-purification process of lactic acid solutions employing an engineered E. coli strain in a membrane bioreactor

 

t (h)

ΤEST 1

ΤEST 2

ΤEST 3

Glucose (g/L)

0

0.61 ± 0.03

1.24 ± 0.03

2.33 ± 0.04

24

0.00 ± 0.00

0.00 ± 0.00

0.98 ± 0.04

48

0.00 ± 0.00

0.00 ± 0.00

0.12 ± 0.03

72

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

AA (g/L)

0

0.54 ± 0.01

1.02 ± 0.02

2.00 ± 0.04

24

0.36 ± 0.07

1.45 ± 0.34

2.43 ± 0.03

48

0.09 ± 0.00

0.18 ± 0.03

2.57 ± 0.08

72

0.00 ± 0.00

0.00 ± 0.00

2.72 ± 0.10

ΟD600

0

0.15 ± 0.00

0.15 ± 0.00

0.15 ± 0.01

24

0.93 ± 0.04

0.66 ± 0.02

0.67 ± 0.02

48

0.81 ± 0.03

1.12 ± 0.02

0.66 ± 0.01

72

0.75 ± 0.08

0.79 ± 0.01

0.56 ± 0.01

pH

0

7.10 ± 0.00

7.10 ± 0.01

7.08 ± 0.02

24

7.65 ± 0.10

6.59 ± 0.01

6.31 ± 0.01

48

8.04 ± 0.05

8.52 ± 0.04

5.82 ± 0.04

72

8.03 ± 0.04

8.89 ± 0.06

5.79 ± 0.04

LA (g/L)

0

5.21 ± 0.22

5.05 ± 0.07

4.97 ± 0.06

24

5.25 ± 0.16

5.16 ± 0.04

5.06 ± 0.05

48

5.15 ± 0.14

5.12 ± 0.08

5.13 ± 0.14

72

5.15 ± 0.11

5.21 ± 0.10

5.18 ± 0.05

TN (ppm)

0

62

62

63

24

32

49

45

48

31

24

48

72

24

18

39

TOC (ppm)

0

2748

3051

3804

24

2136

2693

3228

48

2303

2343

3820

72

2186

2405

3596