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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: Biosynthesis of fragrance 2-phenylethanol from sugars by Pseudomonas putida

Fig. 3

Production of 2-PE by resting cells in the presence of Ehrlich pathway intermediates: l-phenylalanine (l-Phe), phenylacetaldehyde (PA) or phenylpyruvate (PP). CM12-5 (pPE-1) (blue bars) and CM12-5Δgcd (xylABE) (pPE-1) (red bars) cells were grown in the presence of glucose as the C source until reaching a DO660 of 1, the cultures were then washed and concentrated in 1xM9 + 0.5% (w/v) glucose or xylose to reach a DO660 of about 10, and the effect of 1 mM l-Phe PA or PP supplements on 2-PE production was measured after 24 h of incubation. The figure shows the average values of three independent assays

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