Biotechnology for Biofuels and Bioproducts

Table 2 2-PE production from 2G lignocellulosic hydrolysates PCS and PSCS in Pseudomonas putida CM12-5 derivative strains

From: Biosynthesis of fragrance 2-phenylethanol from sugars by Pseudomonas putida

		3% PCS				3% PSCS
		(pPE-1)	(pPHE1) (pPE-1)	PG2E (pPE-1)	Δgcd(xylABE) (pPE-1)	(pPE-1)	(pPHE1) (pPE-1)	PG2E (pPE-1)	Δgcd(xylABE) (pPE-1)
mg L⁻¹	Initial glucose	1630 ± 78	1753 ± 130	1638 ± 107	1562 ± 87	1533 ± 111	1576 ± 99	1576 ± 166	1576 ± 135
	Consumed Glucose	1334 ± 32	1682 ± 149	1456 ± 131	1425 ± 116	1441 ± 121	1548 ± 103	1358 ± 247	1443 ± 195
	Produced 2-PE	86 ± 19	109 ± 17	110 ± 25	85 ± 14	82 ± 15	97 ± 16	93 ± 15	84 ± 11
	Initial xylose	2596 ± 178	2627 ± 59	2600 ± 37	2633 ± 115	2034 ± 203	2305 ± 18	1910 ± 394	2065 ± 205
	Consumed xylose	1990 ± 232	1690 ± 230	1302 ± 431	1978 ± 254	1698 ± 380	1788 ± 102	1367 ± 305	1574 ± 181
mM	Initial glucose	9.05 ± 0.43	9.20 ± 0.72	9.09 ± 0.59	8.67 ± 0.48	8.51 ± 0.61	8.75 ± 0.55	8.75 ± 0.92	8.75 ± 0.75
	Consumed Glucose	7.40 ± 0.18	8.68 ± 0.83	8.08 ± 0.73	7.91 ± 0.64	8.00 ± 0.67	8.59 ± 0.57	7.54 ± 1.37	8.01 ± 1.08
	Produced 2-PE	0.70 ± 0.15	0.82 ± 0.14	0.90 ± 0.21	0.69 ± 0.12	0.67 ± 0.12	0.80 ± 0.13	0.77 ± 0.12	0.69 ± 0.09
	Initial xylose	17.29 ± 1.19	17.50 ± 0.40	17.32 ± 0.25	17.54 ± 0.77	13.55 ± 1.35	15.36 ± 0.12	12.73 ± 2.62	13.76 ± 1.36
	Consumed xylose	13.26 ± 1.55	11.26 ± 1.53	8.67 ± 2.87	13.18 ± 1.69	11.31 ± 2.53	11.91 ± 0.68	9.11 ± 2.03	10.49 ± 1.21
(G) Molar yield (%)		8.85 ± 1.68	9.51 ± 1.82	11.14 ± 1.76	8.80 ± 1.66	8.68 ± 1.28	9.24 ± 0.89	10.17 ± 0.26	8.64 ± 1.06

The assays were carried out as described in M8 minimal medium with 10 mM KNO₃ and 3% (w/v) of hydrolysated PCS or PSCS. Glucose and xylose were determined at the beginning of the assay and 24 h later. The concentration of 2-PE was determined at the end of the assay. The values and standard deviation are the average of three independent assays

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