Biotechnology for Biofuels and Bioproducts

Table 3 2-PE production from corn syrup (CS) in Pseudomonas putida CM12-5 derivative strains

From: Biosynthesis of fragrance 2-phenylethanol from sugars by Pseudomonas putida

		CS 1/80 (v/v)
		(pPE-1)	(pPHE1) (pPE-1)	PG2E (pPE-1)	Δgcd (xylABE) (pPE-1)
mg L⁻¹	Initial glucose	4725 ± 94	4737 ± 95	4722 ± 89	4729 ± 100
	Consumed glucose	2375 ± 48	2117 ± 42	2579 ± 52	2204 ± 44
	Initial fructose	6351 ± 127	6323 ± 162	6318 ± 120	6349 ± 127
	Consumed fructose	429 ± 9	357 ± 7	273 ± 5	474 ± 9
	Produced 2-PE	28.7 ± 0.57	43.8 ± 0.87	84.1 ± 1.68	10.5 ± 0.21
mM	Initial glucose	26 ± 0.52	26 ± 0.53	26 ± 0.52	26 ± 0.52
	Consumed glucose	13 ± 0.26	12 ± 0.24	14 ± 0.28	12 ± 0.24
	Initial fructose	35.3 ± 0.71	35.3 ± 0.70	35.3 ± 0.70	35.3 ± 0.71
	Consumed fructose	2.4 ± 0.05	2.0 ± 0.04	1.5 ± 0.03	2.6 ± 0.05
	Produced 2-PE	0.23 ± 0.01	0.36 ± 0.02	0.69 ± 0.03	0.09 ± 0.01
(G) Molar yield (%)		1.78 ± 0.03	3.05 ± 0.05	4.81 ± 0.09	0.70 ± 0.01

The assays were carried as described in the footnote for Table 3 except that corn syrup was used. Glucose and fructose were determined at t = 0 and 24 h, and 2-PE was measured at the end of the assays. The values and standard deviation are the average of three independent assays

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