Fig. 3

Promotion of lignocellulose utilization efficiency of T. guizhouense by deleting Tgparp or overexpressing Tgadprase under heat stress. a Growth comparison of the wt and mutants inoculated on PDA medium for 72 h at 28 °C and rice straw medium for 48 h at 28 °C and 37 °C. b Hydrolase activities, including FPA, EG, CBH, and XYL, of wt and mutants using rice straw as the sole carbon source under heat stress. c, Biomass of T. guizhouense by quantifying the copies of a 300 bp fragment from T. guizhouense in different treatments under heat stress (values are shown as the log₁₀ conversion of the fragment copies·g⁻¹ FW rice straw-induced cultures). d, e Transcriptional levels of some representative lignocellulase genes and regulatory factors in different treatments based on SWATH analysis. The representative lignocellulase genes included r-Tgegl (endo-1,4-β-glucanase, OPB37031), r-Tgcbh (cellobiohydrolase, OPB45635), r-Tgbgl (β-glucosidase, KKP01743), and r-Tgxyl (endo-1,4-β-xylanase, OPB45659). The regulatory factors included Tgace1 (OPB46882.1), Tgcre1 (OPB38360.1), Tgxyr1 (OPB38038.1), and Tgire (OPB43384.1). Data were calculated from three biological replicates, and error bars represent ± SDs. * P < 0.05, ** P < 0.01, *** P < 0.001. A P-value < 0.05 was regarded as statistically significant