Fig. 6

Localization analysis of lignocellulases-GFP in the ER and extracellular region between wt and ΔTgparp. a Confocal images of hyphae from the lignocellulases-GFP fusion strains after staining with ER-Tracker™ Red to track lignocellulases localization in the ER between wt and ΔTgparp. b Average intensity of lignocellulases-GFP fluorescence in the ER of wt and ΔTgparp after staining hyphae with ER-Tracker™ as markers of ER compartment. Data were calculated from all valid pixels in the ER, and error bars represent ± SEs. c Fluorescence intensities of fermentation supernatant when SSF of different lignocellulase-GFP fusion strains was carried out for 4 days. Data were calculated from three biological replicates. Error bars represent ± SDs. d–e Western blot analysis of lignocellulase-GFPs in the ER (left) and fermentation supernatants (right), and the intensity of Western blotting bands was compared between wt and ΔTgparp. Three independent biological experiments were performed and yielded similar results in each independent biological experiment. Error bars represent ± SDs. * P < 0.05, ** P < 0.01, *** P < 0.001. A P-value < 0.05 was regarded as statistically significant