Fig. 5From: Small GTPase Rab7 is involved in stress adaptation to carbon starvation to ensure the induced cellulase biosynthesis in Trichoderma reeseiTrrab7 knock-down showed no large effect on the secretion of overexpressed EG1. A Extracellular CMCase activities of the culture supernatant from QM9414, QM9414 & Pcdna-eg1, and Ptcu-rab7KD & Pcdna-eg1 strains cultured with 1% (w/v) glucose at indicated time points. Significant differences were determined by a two-tailed student's t test. *, P < 0.05; **P < 0.01; ***P < 0.001. B SDS–PAGE analysis of the culture supernatants of indicated strains cultured with 1% (w/v) glucose. M denotes standard molecular weight protein marker. Culture supernatants (20 μL) were taken at indicated time points and loaded for analysis. As the parental strains QM9414 and Ptcu-rab7KD did not produce significant extracellular proteins with glucose as the sole carbon source, the observed protein band with the highest intensity on SDS–PAGE between 55 and 70 kD was EG1Back to article page