Fig. 6

Overexpression of Xyr1 rescued the defect in cellulase production of Ptcu-rab7^KD with glucose but not Avicel. A, B Quantitative RT-PCR analyses of the relative transcription of rab7 (A) and xyr1 (B) of the QM9414, QM9414 & OExyr1, and Ptcu-rab7^KD & OExyr1 strains cultured with 1% (w/v) glucose at the indicated time points. C Extracellular pNPC hydrolytic activities of the culture supernatant from QM9414, QM9414 & OExyr1, and Ptcu-rab7^KD & OExyr1 strains cultured with 1% (w/v) glucose. D SDS–PAGE analysis of the culture supernatants of the indicated strains cultured with 1% (w/v) glucose. Culture supernatants (20 μL) were taken at the indicated time points and loaded for analysis. M denotes standard molecular weight protein marker. E Extracellular pNPC hydrolytic activities of the culture supernatant of the QM9414, Ptcu-rab7^KD, QM9414 & OExyr1, and Ptcu-rab7^KD & OExyr1 strains cultured with 1% (w/v) Avicel at indicated time points. F Quantitative RT-PCR analyses of the relative transcription of xyr1 cultured with 1% (w/v) Avicel at the indicated time points. Significant differences were determined by a two-tailed student's t test. *, P < 0.05; **P < 0.01; ***P < 0.001