Fig. 8

Overexpression of Snf1 partially rescued the cellulase production defect of Ptcu-rab7^KD on Avicel. A Quantitative RT-PCR analyses of the relative transcription of snf1 of QM9414, Ptcu-rab7^KD, and Ptcu-rab7^KD & OEsnf1 strains after induction with 1% (w/v) Avicel. Extracellular pNPC (B), pNPG (C), and CMCase activities (D) of the culture supernatant from QM9414, Ptcu-rab7^KD, and Ptcu-rab7^KD & OEsnf1 strains cultured with 1% (w/v) Avicel at the indicated points. E SDS–PAGE analysis of the culture supernatants of QM9414, Ptcu-rab7^KD, and Ptcu-rab7^KD & OEsnf1 strains cultured with 1% (w/v) Avicel. Equal amounts (20 μL) of culture supernatant were loaded for all strains. M denotes standard molecular weight protein marker. F Quantitative RT-PCR analyses of the relative transcription of cbh1 after induction with 1% (w/v) Avicel. Significant differences were determined by a two-tailed student's t test. *, P < 0.05; **P < 0.01; *** P < 0.001