The role of acetyl xylan esterase in the solubilization of xylan and enzymatic hydrolysis of wheat straw and giant reed
© Zhang et al; licensee BioMed Central Ltd. 2011
Received: 31 August 2011
Accepted: 20 December 2011
Published: 20 December 2011
Due to the complexity of lignocellulosic materials, a complete enzymatic hydrolysis into fermentable sugars requires a variety of cellulolytic and xylanolytic enzymes. Addition of xylanases has been shown to significantly improve the performance of cellulases and to increase cellulose hydrolysis by solubilizing xylans in lignocellulosic materials. The goal of this work was to investigate the effect of acetyl xylan esterase (AXE) originating from Trichoderma reesei on xylan solubilization and enzymatic hydrolysis of cellulose.
The solubilization of xylan in pretreated wheat straw and giant reed (Arundo donax) by xylanolytic enzymes and the impact of the sequential or simultaneous solubilization of xylan on the hydrolysis of cellulose by purified enzymes were investigated. The results showed that the removal of acetyl groups in xylan by AXE increased the accessibility of xylan to xylanase and improved the hydrolysis of xylan in pretreated wheat straw and giant reed. Solubilization of xylan led to an increased accessibility of cellulose to cellulases and thereby increased the hydrolysis extent of cellulose. A clear synergistic effect between cellulases and xylanolytic enzymes was observed. The highest hydrolysis yield of cellulose was obtained with a simultaneous use of cellulases, xylanase and AXE, indicating the presence of acetylated xylan within the cellulose matrix. Acetylated xylobiose and acetylated xylotriose were produced from xylan without AXE, as confirmed by atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry.
The results in this paper demonstrate that supplementation of xylanase with AXE enhances the solubilization of xylan to some extent and, consequently, increases the subsequent hydrolysis of cellulose. The highest hydrolysis yield was, however, obtained by simultaneous hydrolysis of xylan and cellulose, indicating a layered structure of cellulose and xylan chains in the cell wall substrate. AXE has an important role in the hydrolysis of lignocellulosic materials containing acetylated xylan.
Plant cell walls consist of three major polymers: cellulose, hemicelluloses and lignin. Cellulose, the most abundant constituent of the plant cell wall, is a homopolysaccharide composed entirely of D-glucose linked together by β-1,4-glucosidic bonds. Xylans, the main hemicelluloses in hardwoods and annual plants, consist of a linear backbone of β-(1→4)-D-xylopyranosyl residues, substituted by α-L-arabinofuranosyl units in the positions of 2-O and/or 3-O, by 4-O-methyl-glucopyranosyl uronic acid in the position of 2-O, and/or by acetyl groups in the positions of 2-O and/or 3-O . Furthermore, some of the arabinofuranosyl units may be esterified with ferulic or p-coumaric acids .
Complete hydrolysis of lignocellulosic materials to monosaccharides for fermentation to fuels or chemicals can be accomplished by acid hydrolysis, but enzymatic hydrolysis is preferred due to minimization of the formation of byproducts that inhibit the microbial conversion. Due to the complexity of lignocellulosic materials, a complete enzymatic hydrolysis into fermentable monosaccharides requires a variety of cellulolytic and xylanolytic enzymes. Efficient cellulose hydrolysis requires the cooperative action of endoglucanases (E.C. 18.104.22.168), which hydrolyze the cellulose polymer internally, exposing reducing and non-reducing ends, and exoglucanases or cellobiohydrolases (E.C. 22.214.171.124), which act on the reducing or non-reducing ends, releasing mainly cellobiose. The cellulose hydrolysis process is finalized through the action of β-glucosidase (E.C. 126.96.36.199), which cleaves cello-oligosaccharides into two molecules of glucose [3, 4]. Given the diversity of xylan structures, a complete hydrolysis of xylan involves the synergistic action of main chain degrading enzymes, including endo-β-1,4-xylanases (EC 188.8.131.52) and β-D-xylosidases (EC 184.108.40.206), and side group cleaving enzymes, including α-L-arabinofuranosidases (EC 220.127.116.11), α-glucuronidases (EC 18.104.22.168), acetyl xylan esterases (AXEs) (EC 22.214.171.124), and feruloyl esterases (EC 126.96.36.199).
In lignocellulosic matrices, xylan is closely associated with the cellulose fibrils, as well as lignin, and does to some extent cover the fiber surfaces, thereby limiting the access of cellulases to the cellulose surface . It has been reported that enzymatic removal of xylan enhances cellulose hydrolysis by removing xylan covering or entrapping cellulose [6, 7]. The addition of xylanases (XYLs) has been shown to significantly improve the performance of cellulases and to increase the cellulose conversion of many lignocellulosic materials [8–11]. Thus, the solubilization of xylan in lignocellulosic materials plays an important role in efficient enzymatic hydrolysis.
Hydrolysis of xylan can be enhanced by the removal of xylan side groups; it has been reported that the hydrolysis of isolated hardwood xylans by XYLs was restricted by increasing the degree of acetylation of the xylans . Chemical deacetylation of xylans of aspen wood and wheat straw increased the enzymatic solubilization of xylans and consequently enhanced cellulose accessibility . The improvement of xylan hydrolysis by the concerted action of endoxylanase and AXE has been observed and resulting in further synergistic improvements in cellulose hydrolysis . A relatively linear relationship was observed between the removal of acetyl groups and the release of xylose in the hydrolysis of different corn stover substrates with both endoxylanase and AXE . After pretreatments using hydrothermal or steam explosion treatment of annual plants, some of the substituents, such as acetyl residues, may remain in xylans and hinder the action of XYLs during the enzymatic hydrolysis. In addition, the presence of lignin-carbohydrate complexes between arabinose residues and ferulic or p-coumaric acid formed through etherification or esterification has been suggested. Synergism between α-L-arabinofuranosidases and XYLs in the hydrolysis of xylan and agricultural residues has also been reported by Raweesri et al. . α-L-arabinofuranosidases from Aspergillus niger and endoxylanase from A. nidulans synergistically enhanced the hydrolysis of steam exploded wheat straw by cellulases . In order to further elucidate the enzymatic hydrolysis of complex lignocellulosic materials, it is necessary to investigate the solubilization of xylan by xylan-acting enzymes and the consequential effect on cellulose hydrolysis.
In this work, the synergistic action of xylanolytic enzymes in the solubilization of xylan and the subsequent enhancement of cellulose hydrolysis was carried out sequentially or simultaneously with pure cellulases on pretreated wheat straw and giant reed (Arundo donax). The role of xylan solubilization with xylanolytic enzymes was investigated by analyzing the carbohydrates, including monosaccharides, disaccharides and xylo-oligosaccharides, released from the substrates.
Results and discussion
Solubilization of xylan by xylanolytic enzymes
Carbohydrates released from pretreated wheat straw and giant reed by xylan-acting enzymes after 24 h hydrolysis in 50 mM sodium citrate buffer at pH 5.0 and 45°C
There was no obvious synergistic effect observed between XYL and α-arabinofuranosidase, most probably due to the low content of the residual L-arabinofuranosyl side groups in the xylans of wheat straw (0.05%) and giant reed (0.3%). The synergy between XYL and arabinofuranosidase in the degradation of arabinoxylan, rich in arabinose substituents, has previously been described by Sørensen et al. [21, 22]. The hydrolysis degree of xylans in the pretreated substrates reached 11.3% and 13.7%, respectively, when AXE was added to XYL (Table 1). Supplementation of XYL with AXE caused a clear increase in the amount of xylose and xylobiose, whereas no xylotriose or xylotetraose were detected. After the supplementation of XYL with AXE, 1.1% and 2.6% of acetyl groups (of the original xylan in the substrate) were released from wheat straw and giant reed, respectively. The acetyl groups in lignocellulosic materials are known to restrict the action of XYLs and limit the solubilization of xylan in the substrates [13, 15, 23]. The synergy between esterase and XYLs has been reported previously [24, 25]. Chemical or enzymatic removal of acetyl groups has been shown to increase the availability of the xylan backbone to XYLs and improve the hydrolysis [13, 15]. In the present work, the addition of AXE enhanced the solubilization of xylan to some extent, which indicated that AXE removed some acetyl groups in xylan and increased the accessibility of xylan to XYL. The AXE from Trichoderma reesei has high specificity for acetylated xylan and it has no activity towards acetylated galactoglucomannan or phenolic substituents from wheat straw xylan .
Influence of xylan solubilization on cellulose hydrolysis
Simultaneous hydrolysis of xylan and cellulose
Carbohydrates released from pretreated wheat straw and giant reed after 48 h hydrolysis by cellulases and xylan-acting enzymes in 50 mM sodium citrate buffer at pH 5.0 and 45°C
As expected from the results above, there was no obvious enhancement of the simultaneous action of CEL plus XYL on the pretreated wheat straw and giant reed by addition of α-arabinofuranosidase (Figure 2). The highest contents of carbohydrates were released from the pretreated substrates by the combined action of CEL plus XYL plus AXE (Table 2); a consequence of the most extensive xylan solubilization. The amount of acetyl group released from the substrates after supplementation of CEL and XYL with AXE was 4.5% and 3.6% of the original xylan in the pretreated wheat straw and giant reed, respectively. The addition of AXE improved the release of acetyl groups from xylans and subsequently increased the solubilization of xylans, leading to an enhanced action of CEL via improvement of cellulose accessibility and solubilization. The results were in good agreement with reports on the combination of endoxylanase and AXE in increasing the xylan conversion, and consequently the cellulose conversion to glucose by cellulolytic enzymes [14, 15].
The amount of xylose, xylobiose and xylotriose released by the combination of cellulases and xylanolytic enzymes was higher than released by corresponding xylanolytic enzymes alone (Tables 1 and 2). Obviously, hydrolysis with xylanolytic enzymes with the addition of CEL increased the solubilization of xylan in the substrates. The highest hydrolysis degrees of xylans in the pretreated wheat straw and giant reed were 64.3% and 29.8% (compared with 11.3% and 13.7%, respectively, in the absence of cellulose hydrolysis). The xylans in the substrates were not fully solubilized due to structural limitations caused by associations of hemicelluloses, cellulose and lignin in the plant cell wall . The degree of cellulose hydrolysis by the combination of CEL plus XYL with AXE followed the same trend, thus being significantly higher (55.6%) in wheat straw and in giant reed (28.9%) (Table 2). The final degree of cellulose hydrolysis may also have been limited by the number of cellulases chosen in this work, excluding some of the endoglucanases and other essential minor cellulolytic enzymes.
Action of AXE in enzymatic hydrolysis
Solubilization of xylan by xylanolytic enzymes before the addition of pure cellulases clearly increased the hydrolysis yield of cellulose in pretreated wheat straw and giant reed. Supplementation of XYL with AXE enhanced the solubilization of xylan only slightly and had a minor effect on the subsequent hydrolysis of cellulose. The hydrolysis of xylan, however, was dramatically increased along with simultaneous hydrolysis of cellulose, indicating a layered structure of cellulose and xylan chains occurring alternately in the cell wall substrate. Also, the total hydrolysis yield was significantly increased by the simultaneous action of xylanases and cellulases. A linear relationship between the hydrolysis yields of xylan and cellulose was thus obtained, which showed clear synergism between CEL and xylanolytic enzymes in the hydrolysis of wheat straw and giant reed. The presence of AXE clearly increased the simultaneous solubilization and hydrolysis of xylan and cellulose. The XYL used produced xylobiose as the main hydrolysis product. No xylotriose was detected in the hydrolysates from the substrates after the addition of AXE, and more xylose and xylobiose was detected. The results indicated that AXE could remove acetyl group from xylan in the substrates and released xylan fragments, creating new sites for XYL action and thus leading to the formation of shorter carbohydrates and increased xylan solubilization. The data in this work supports the finding that AXE has an important role in the hydrolysis of pretreated wheat straw and giant reed.
The monosaccharides, D-xylose and D-glucose were purchased from Merck (Darmstadt, Germany) and 1,4-β-D-xylobiose, 1,4-β-D-xylotriose, 1,4-β-D-xylotetraose and cellobiose were from Megazyme (Bray, Ireland). All other chemicals used were analytical grade.
Hydrothermally pretreated wheat straw was a kind gift from Inbicon (Fredericia, Denmark). The carbohydrate composition of the wheat straw as monosaccharides was glucose 65.4%, xylose 3.6% and arabinose 0.05% of dry matter (DM). The pretreated giant reed (A. donax) was kindly provided by Chemtex (Tortona, Italy). The carbohydrate composition of the giant reed as monosaccharides was glucose 49.8%, xylose 7.7% and arabinose 0.3% of DM. The amounts of acetyl groups were 0.12% and 0.44% of DM in the pretreated wheat straw and giant reed, respectively.
Cellobiohydrolases CBH I (Cel 7A), CBH II (Cel 6A), and endoglucanase EG II (Cel 5A) from T. reesei were purified as described by Suurnäkki et al. . A. niger β-glucosidase (βG) (Cel 3A) was purified from Novozyme 188 (Novozymes A/S, Bagsvaerd, Denmark) according to Sipos et al. . XYL (family 10) from Thermoascus aurantiacus was a kind gift from ROAL and was partially purified from the preparation containing XYL, heterologously produced by the genetically modified T. reesei strain . AXE from T. reesei was purified as described by Sundberg and Poutanen . The α-L-arabinofuranosidase was from Novozymes. XYL activity was assayed using birchwood glucuronoxylan as a substrate in 50 mM sodium citrate buffer (pH 5.0) according to the method of Bailey et al. . AXE activity was assayed using p-nitrophenyl acetate as a substrate  and α-L-arabinofuranosidase activity using p-nitrophenyl-α-L-arabinofuranoside as a substrate . Protein was quantified by the Lowry method, using BSA (St. Louis, MO, Sigma Chemical Co., USA) as standard .
In order to investigate the solubilization of xylan in the pretreated wheat straw and giant reed by different xylanolytic enzyme mixtures and examine the influence of xylan solubilization on further hydrolysis of substrates by cellulases, the enzymatic hydrolysis experiments were carried out in two steps. In the prehydrolysis step, the hydrolysis of the pretreated wheat straw and giant reed was carried out by the different xylanolytic enzyme mixtures in a working volume of 2.5 mL in 50 mM sodium citrate buffer (pH 5.0) containing 0.02% NaN3 at 45°C. All hydrolysis experiments were carried out in duplicates. The DM content of the substrate was 2%. XYL was dosed at 5 000 nkat/g substrate (0.36 mg protein/g substrate), and AXE and α-L-arabinofuranosidase were both dosed at 500 nkat/g substrate (0.17 mg protein/g substrate for α-L-arabinofuranosidase). The dosages of different enzymes used were always the same throughout the experiments unless otherwise noted. After 24 h of hydrolysis, all samples were withdrawn and boiled for 10 min to stop the enzymatic hydrolysis. After cooling, the samples were washed three times with Milli-Q-water (Milli-Q-plus, Millipore, Billerica, MA, USA) and the supernatants were combined for analysis. In the second step, the hydrolysis residues were further hydrolyzed by the CEL containing CBHI 6.5 mg, CBHII 1.5 mg, EGII 2.0 mg and βG 500 nkat/g substrate in the same conditions as described previously. Samples were withdrawn at 24 h and 48 h and boiled for 10 min to stop the enzymatic hydrolysis.
The simultaneous hydrolysis of the pretreated wheat straw and giant reed by cellulolytic enzymes (CEL) and different xylanolytic enzymes was carried out as described above. The DM content of substrate was 2%. CEL (CBHI plus CBHII plus EGII plus βG) was dosed and loaded in all samples as described earlier and XYL, AXE and α-L-arabinofuranosidase were added to examine the synergistic enhancement between cellulases and different xylanolytic enzymes. Samples were withdrawn at 24 h and 48 h and boiled for 10 min to stop the enzymatic hydrolysis. All samples were centrifuged and the supernatants were analyzed for reducing sugars. Two replicate tests were carried out in all hydrolysis experiments and average values were presented. The reducing sugar concentrations showed a relative standard error of less than 4%. Monosaccharides, disaccharides and oligosaccharides were analyzed by HPAEC-PAD. The relative standard errors were determined for the standards and were shown to be consistently below 1%.
Analysis of carbohydrates
The amount of reducing sugars liberated was determined using the dinitrosalicylic acid method with xylose or glucose as standard . Monosaccharides in the hydrolysates were analyzed using HPAEC-PAD system equipped with Waters 2707 autosampler, Waters 515 HPLC pumps and Waters 2465 pulsed amperometric detector using Empower 2 software for instrument control and data analysis (Waters Corporation, Milford, MA, USA). An analytical CarboPac PA-1 column (4.0 mm ID × 250 mm) in combination with a CarboPac PA-1 guard column (4.0 mm × 50 mm) (Dionex, Sunnyvale, USA) was used. For the analysis of monosaccharides, the eluents for gradient analysis were H2O and 0.2 M NaOH with a total flow rate of 1 mL/min with post column addition of 300 mM NaOH at a flow rate of 0.3 mL/min. D-glucose, D-xylose, and D-arabinose (Merck, Darmstadt, Germany) were used as external standards .
The analytical CarboPac PA-100 column (250 mm × 4 mm) and the guard column PA-100 (25 mm × 3 mm) (Dionex) were used for disaccharide and oligosaccharide analysis. The eluents for gradient analysis were 100 mM NaOH and 100 mM NaOH/1 M NaOAc at a flow rate of 1 mL/minute as described by Rantanen et al. . It should be observed that the acetyl substituents in the oligosaccharides were removed during the HPAEC-PAD analysis due to the alkaline eluent. 1,4-β-D-cellobiose, 1,4-β-D-xylobiose, 1,4-β-D-xylotriose and 1,4-β-D-xylotetraose (Megazyme, Bray, Ireland) were used as external standards.
Analysis of acetyl groups
The content of acetyl groups of the substrates was analyzed using the Megazyme Acetic Assay Kit (K-ACET) after acid hydrolysis of the samples.
The hydrolysates of giant reed were desalted and concentrated using Hypersep Hypercarb Porous Graphitized Carbon columns (Thermo Scientific, Waltham, USA), according to the established protocols by Packer et al. . The oligosaccharides were eluted from the column by 50% (v/v) CH3CN in 0.05% (v/v) trifluoroacetic acid. Eluted oligosaccharides were dried and dissolved in 30 μL of Mili-Q-water. The 2,5-dihydroxybenzoic acid (DHB) solution (10 mg/mL) was employed as a matrix for AP-MALDI-ITMS measurement. The 10 mg DHB was dissolved in 1 mL CH3CN/Milli-Q-water (3:7, v/v). The oligosaccharides were crystallized on a target plate by mixing 1 μL of the sample with 1 μL of the DHB solution, followed by drying under a constant stream of warm air. The AP-MALDI-ITMS was operated according to the protocols described by Chong et al. .
List of abbreviations
atmospheric pressure matrix assisted laser desorption/ionization ion trap mass spectrometer
acetyl xylan esterase
bovine serum albumin
mixture of cellulose-acting enzymes
high-performance anion exchange chromatography coupled with pulsed amperometric detection
The authors acknowledge the financial support for this research from HYPE project (project number: 213139) and NEMO project (project number: 222699). This work was also supported by the Natural Science Foundation of Shaanxi Province (project number: 2011JQ3007) and Northwest A&F University Key Research Project for Young Staff. The authors are grateful to Laura Huikko and Sun-Li Chong (University of Helsinki, Finland) for assistance in the HPAEC-PAD and performing the AP-MALDI-IT-MS analysis. Inbicon (Fredericia, Denmark) and Chemtex (Italy) are gratefully acknowledged for providing pretreated wheat straw and pretreated giant reed, respectively. Roal Oy (Rajamäki, Finland) is also gratefully acknowledged for providing some enzymes.
- Aspinall GO: Structural chemistry of the hemicelluloses. Adv Carbohydr Chem. 1959, 14: 429-468.Google Scholar
- Mueller-Harvey I, Hartley RD, Harris PJ, Curzon EH: Linkage of p-coumaroyl and feruloyl groups to cell-wall polysaccharides of barley straw. Carbohydr Res. 1986, 148: 71-85. 10.1016/0008-6215(86)80038-6.View ArticleGoogle Scholar
- Himmel ME, Adney WS, Baker JO, Nieves RA, Thomas SR: Cellulases: structure, function, and applications. Handbook on bioethanol: Production and utilization. Edited by: Wyman CE. 1996, Washington DC: Taylor and Francis, 143-161.Google Scholar
- Lynd LR, Weimer PJ, Zyl WH, Pretorius IS: Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev. 2002, 66: 506-524. 10.1128/MMBR.66.3.506-577.2002.View ArticleGoogle Scholar
- Saha BC: Hemicellulose bioconversion. J Ind Microbiol Biotechnol. 2003, 30: 279-291. 10.1007/s10295-003-0049-x.View ArticleGoogle Scholar
- Yang B, Wyman CE: Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose. Biotechnol Bioeng. 2004, 86: 88-95. 10.1002/bit.20043.View ArticleGoogle Scholar
- Öhgren K, Bura R, Saddler J, Zacchi G: Effect of hemicellulose and lignin removal on enzymatic hydrolysis of steam pretreated corn stover. Bioresour Technol. 2007, 98: 2503-2510. 10.1016/j.biortech.2006.09.003.View ArticleGoogle Scholar
- Berlin A, Gilkes N, Kilburn D, Bura R, Markov A, Skomarovsky A, Okunev O, Gusakov A, Maximenko V, Gregg D, Sinitsyn A, Saddler J: Evaluation of novel fungal cellulase preparations for ability to hydrolyze softwood substrates - evidence for the role of accessory enzymes. Enzyme Microb Technol. 2005, 37: 175-184. 10.1016/j.enzmictec.2005.01.039.View ArticleGoogle Scholar
- Berlin A, Gilkes N, Kilburn D, Maximenko V, Bura R, Markov A, Skomarovsky A, Gusakov A, Sinitsyn A, Okunev O, Solovieva I, Saddler JN: Evaluation of cellulase preparations for hydrolysis of hardwood substrates. Appl Biochem Biotechnol. 2006, 129-132: 528-545.View ArticleGoogle Scholar
- García-Aparicio MP, Ballesteros M, Manzanares P, Ballesteros I, González A, Negro MJ: Xylanase contribution to the efficiency of cellulose enzymatic hydrolysis of barley straw. Appl Biochem Biotechnol. 2007, 136-140: 353-366.Google Scholar
- Kumar R, Wyman CE: Effect of xylanase supplementation of cellulase on digestion of corn stover solids prepared by leading pretreatment technologies. Bioresour Technol. 2009, 100: 4203-4213. 10.1016/j.biortech.2008.11.057.View ArticleGoogle Scholar
- Poutanen K, Rättö M, Puls J, Viikari L: Evaluation of different microbial xylanolytic system. J Biotechnol. 1987, 6: 49-60. 10.1016/0168-1656(87)90045-9.View ArticleGoogle Scholar
- Grohmann K, Mitchell DJ, Himmel ME, Dale BE, Schroeder HA: The role of ester groups in resistance of plant cell wall polysaccharides to enzymatic hydrolysis. Appl Biochem Biotechnol. 1989, 20/21: 45-61. 10.1007/BF02936472.View ArticleGoogle Scholar
- Selig MJ, Knoshaug EP, Adney WS, Himmel ME, Decker SR: Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities. Bioresour Technol. 2008, 99: 4997-5005. 10.1016/j.biortech.2007.09.064.View ArticleGoogle Scholar
- Selig MJ, Adney WS, Himmel ME, Decker SR: The impact of cell wall acetylation on corn stover hydrolysis by cellulolytic and xylanolytic enzymes. Cellulose. 2009, 16: 711-722. 10.1007/s10570-009-9322-0.View ArticleGoogle Scholar
- Raweesri P, Riangrungrojana P, Pinphanichakarn P: α -L-arabinofuranosidase from Streptomyces sp. PC22: Purification, characterization and its synergistic action with xylanolytic enzymes in the degradation of xylan and agricultural residues. Bioresour Technol. 2008, 99: 8981-8986. 10.1016/j.biortech.2008.05.016.View ArticleGoogle Scholar
- Alvira P, Negro MJ, Ballesteros M: Effect of endoxylanase and α-L-arabinofuranosidases supplementation on the enzymatic hydrolysis of steam exploded wheat straw. Bioresour Technol. 2011, 102: 4552-4558. 10.1016/j.biortech.2010.12.112.View ArticleGoogle Scholar
- Viikari L, Kantelinen A, Buchert J, Puls J: Enzymatic accessibility of xylans in lignocellulosic materials. Appl Microbiol Biotechnol. 1994, 41: 124-129. 10.1007/BF00166093.View ArticleGoogle Scholar
- Zilliox C, Debeire P: Hydrolysis of wheat straw by a thermostable endoxylanase: adsorption and kinetic studies. Enzyme Microbiol Technol. 1998, 22: 58-63. 10.1016/S0141-0229(97)00105-1.View ArticleGoogle Scholar
- Zhang J, Siika-aho M, Puranen T, Tang M, Tenkanen M, Viikari L: Thermostable recombinant xylanases from Nonomuraea flexuosa and Thermoascus aurantiacus show distinct properties in the hydrolysis of xylans and pretreated wheat straw. Biotechnol Biofuels. 2011, 4: 12-10.1186/1754-6834-4-12.View ArticleGoogle Scholar
- Sørensen HR, Meyer AS, Pedersen S: Enzymatic hydrolysis of water-soluble wheat arabinoxylan. 1. Synergy between α-L-arabinofuranosidases, endo-1,4-β-xylanase, and β-xylosidase activities. Biotechnol Bioeng. 2003, 81: 726-731. 10.1002/bit.10519.View ArticleGoogle Scholar
- Sørensen HR, Pedersen S, Meyer AS: Synergistic enzyme mechanisms and effects of sequential enzyme additions on degradation of water insoluble wheat arabinoxylan. Enzyme Microbiol Technol. 2007, 40: 908-918. 10.1016/j.enzmictec.2006.07.026.View ArticleGoogle Scholar
- Mitchell DJ, Grohmann K, Himmel ME, Dale BE, Schroeder HA: Effect of the degree of acetylation on the enzymatic digestion of acetylated xylan. J Wood Chem. 1990, 10: 111-121. 10.1080/02773819008050230.View ArticleGoogle Scholar
- Biely P, MacKenzie CR, Puls J, Schneider H: Cooperativity of esterases and xylanases in the enzymatic degradation of acetyl xylan. Nature Biotechnol. 1986, 4: 731-733. 10.1038/nbt0886-731.View ArticleGoogle Scholar
- Tenkanen M, Siika-aho M, Hausalo T, Puls J, Viikari L: Synergism between xylanolytic enzymes of Trichoderma reesei in the degradation of acetyl-4-O-methylglucuronoxylan. Biotechnology in the pulp and paper industry: advances in applied and fundamental research. Edited by: Srebotnik E, Messner K. 1996, Vienna: Facultas-Universitatsverlog, 503-508.Google Scholar
- Tenkanen M: Action of Trichoderma reesei and Asperginus oryzae esterases in the deacetylation of hemicelluloses. Biotechnol Appl Biochem. 1998, 27: 19-24.View ArticleGoogle Scholar
- Berlin A, Maximenko V, Gilkes N, Saddler J: Optimization of enzyme complexes for lignocellulose hydrolysis. Biotechnol Bioeng. 2007, 97: 287-296. 10.1002/bit.21238.View ArticleGoogle Scholar
- Kumar R, Wyman CE: Effects of cellulase and xylanase enzymes on the deconstruction of solid from pretreatment of poplar by leading technologies. Biotechnol Prog. 2009, 25: 302-314. 10.1002/btpr.102.View ArticleGoogle Scholar
- Poutanen K, Sundberg M, Korte H, Puls J: Deacetylation of xylans by acetyl esterases of Trichoderma reesei. Appl Microbiol Biotechnol. 1990, 33: 506-510.Google Scholar
- Biely P, Vršanská M, Tenkanen M, Kluepfel D: Endo-β-1,4-xylanase families: differences in catalytic properties. J Biotechnol. 1997, 57: 151-166. 10.1016/S0168-1656(97)00096-5.View ArticleGoogle Scholar
- Biely P, Mastihubová M, Tenkanen M, Eyzaguirre J, Li XL, Vršanská M: Action of xylan deacetylating enzymes on monoacetyl derivatives of 4-nitrophenyl glycosides of β-D-xylopyranose and α-L-arabinofuranose. J Biotechnol. 2011, 151: 137-142. 10.1016/j.jbiotec.2010.10.074.View ArticleGoogle Scholar
- Suurnäkki A, Tenkanen M, Siika-aho M, Niku-Paavola ML, Viikari L, Buchert J: Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp. Cellulose. 2000, 7: 189-209. 10.1023/A:1009280109519.View ArticleGoogle Scholar
- Sipos B, Benko Z, Dienes D, Réczey K, Viikari L, Siika-aho M: Characterisation of specific activities and hydrolytic properties of cell-wall degrading enzymes produced by Trichoderma reesei Rut C30 on different carbon sources. Appl Biochem Biotechnol. 2010, 161: 347-364. 10.1007/s12010-009-8824-4.View ArticleGoogle Scholar
- Vehmaanperä J, Alapuranen N, Puranen T, Siika-aho M, Kallio J, Hooman S, Voutilainen S, Halonen T, Viikari L: Treatment of cellulosic material and enzymes useful therein. Pat appl. FI20051318, WO 2007071818, Priority 22.12.2005
- Sundberg M, Poutanen K: Purification and properties of two acetyl xylan esterases of Trichoderma reesei. Biotechnol Appl Biochem. 1991, 13: 1-11.Google Scholar
- Bailey MJ, Biely P, Poutanen K: Interlaboratory testing of methods for assay of xylanase activity. J Biotechnol. 1992, 23: 257-270. 10.1016/0168-1656(92)90074-J.View ArticleGoogle Scholar
- Lowry OH, Roseborough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem. 1951, 193: 265-275.Google Scholar
- Miller GL: Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem. 1959, 31: 4228-Google Scholar
- Pakarinen A, Maijala P, Stoddard FL, Santanen A, Tuomainen P, Kymäläinen M, Viikari L: Evaluation of annual bioenergy crops in the boreal zone for biogas and ethanol production. Biomass Bioenergy. 2011, 35: 3071-3078. 10.1016/j.biombioe.2011.04.022.View ArticleGoogle Scholar
- Rantanen H, Virkki L, Tuomainen P, Kabel M, Schols H, Tenkanen M: Preparation of arabinoxylobiose from rye xylan using family 10 Aspergillus aculeatus endo-1,4-b-D-xylanase. Carbohydr Res. 2007, 68: 350-359.View ArticleGoogle Scholar
- Packer NH, Lawson MA, Jardine DR, Redmond JW: A general approach to desalting oligosaccharides released from glycoproteins. Glycoconj J. 1998, 15: 737-747. 10.1023/A:1006983125913.View ArticleGoogle Scholar
- Chong SL, Nissilä T, Ketola RA, Koutaniemi S, Maceluch MD, Mellerowicz EJ, Tenkanen M, Tuomainen P: Feasibility of using atmospheric pressure matrix-assisted laser desorption/ionization with ion trap mass spectrometry in the analysis of acetylated xylooligosaccharides derived from hardwoods and Arabidopsis thaliana. Anal Bioanal Chem. 2011, 401: 2995-3009. 10.1007/s00216-011-5370-z.View ArticleGoogle Scholar
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