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Table 2 Comparing extents of cellulose hydrolysis after 24 hours at 38°C.

From: Implications of cellobiohydrolase glycosylation for use in biomass conversion

   On BC   On PASC  
Enzyme Modification 1Cellulose Hydrolysis (%) Std. dev. 1Cellulose Hydrolysis (%) Std. dev.
Cel7A None 12.9 0.6 69.0 2.1
  3CD-only 3.7 0.2 10.4 1.4
  4PNGaseF treated #5.6 0.2 #31.4 1.6
rCel7A None 9.2 2.0 26.3 4.1
  3CD-only 3.4 0.3 5.5 1.5
  4PNGaseF treated #5.1 0.1 #26.9 0.5
  1. 1Data for the intact and CD forms are also plotted in Figure 6 and Figure 7.
  2. 3Purified from papain digests (see Materials and Methods).
  3. 4Deglycosylated by PNGaseF under native conditions (see Materials and Methods).
  4. #Activity of deglycosylated enzyme may have been compromised by the deglycosylation protocol and cannot be directly compared to the activity of the intact enzyme.