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Table 2 Comparing extents of cellulose hydrolysis after 24 hours at 38°C.

From: Implications of cellobiohydrolase glycosylation for use in biomass conversion

  

On BC

 

On PASC

 

Enzyme

Modification

1Cellulose Hydrolysis (%)

Std. dev.

1Cellulose Hydrolysis (%)

Std. dev.

Cel7A

None

12.9

0.6

69.0

2.1

 

3CD-only

3.7

0.2

10.4

1.4

 

4PNGaseF treated

#5.6

0.2

#31.4

1.6

rCel7A

None

9.2

2.0

26.3

4.1

 

3CD-only

3.4

0.3

5.5

1.5

 

4PNGaseF treated

#5.1

0.1

#26.9

0.5

  1. 1Data for the intact and CD forms are also plotted in Figure 6 and Figure 7.
  2. 3Purified from papain digests (see Materials and Methods).
  3. 4Deglycosylated by PNGaseF under native conditions (see Materials and Methods).
  4. #Activity of deglycosylated enzyme may have been compromised by the deglycosylation protocol and cannot be directly compared to the activity of the intact enzyme.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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