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  1. Content type: Research

    Microalgal starch is regarded as a promising alternative to crop-based starch for biorefinery such as the production of biofuels and bio-based chemicals. The single or separate use of inorganic carbon source, ...

    Authors: Man Qi, Changhong Yao, Binhuan Sun, Xupeng Cao, Qiang Fei, Bobo Liang, Wenyi Ran, Qi Xiang, Yongkui Zhang and Xianqiu Lan

    Citation: Biotechnology for Biofuels 2019 12:184

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  2. Content type: Research

    Efficient biotechnological conversion of lignocellulosic biomass to valuable products, such as transportation biofuels, is ecologically attractive, yet requires substantially improved mechanistic understanding...

    Authors: Zhenxing Ren, Wuxin You, Shasha Wu, Ansgar Poetsch and Chenggang Xu

    Citation: Biotechnology for Biofuels 2019 12:183

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  3. Content type: Research

    Decreasing fossil fuels and its impact on global warming have led to an increasing demand for its replacement by sustainable renewable biofuels. Microalgae may offer a potential feedstock for renewable biofuel...

    Authors: Kashif Mohd Shaikh, Asha Arumugam Nesamma, Malik Zainul Abdin and Pannaga Pavan Jutur

    Citation: Biotechnology for Biofuels 2019 12:182

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  4. Content type: Research

    Dedicated lignocellulosic feedstock from grass crops for biofuel production is extensively increasing. However, the access to fermentable cell wall sugars by carbohydrate degrading enzymes is impeded by lignin...

    Authors: Philippe Le Bris, Yin Wang, Clément Barbereau, Sébastien Antelme, Laurent Cézard, Frédéric Legée, Angelina D’Orlando, Marion Dalmais, Abdelhafid Bendahmane, Mathias Schuetz, Lacey Samuels, Catherine Lapierre and Richard Sibout

    Citation: Biotechnology for Biofuels 2019 12:181

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  5. Content type: Research

    DNA assembly is an essential technique enabling metabolic engineering and synthetic biology. Combining novel DNA assembly technologies with rational metabolic engineering can facilitate the construction of mic...

    Authors: Shuwen Liu, Haihan Xiao, Fangfang Zhang, Zheng Lu, Yun Zhang, Aihua Deng, Zhongcai Li, Cui Yang and Tingyi Wen

    Citation: Biotechnology for Biofuels 2019 12:180

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  6. Content type: Research

    Countercurrent saccharification is a promising way to minimize enzyme loading while obtaining high conversions and product concentrations. However, in countercurrent saccharification experiments, 3–4 months ar...

    Authors: Chao Liang, Chao Gu, M. Nazmul Karim and Mark Holtzapple

    Citation: Biotechnology for Biofuels 2019 12:179

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  7. Content type: Research

    Algal harvesting is a major cost which increases biofuel production cost. Algal biofuels are widely studied as third-generation biofuel. However, they are yet not viable because of its high production cost whi...

    Authors: Arghya Bhattacharya, Megha Mathur, Pushpendar Kumar and Anushree Malik

    Citation: Biotechnology for Biofuels 2019 12:178

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  8. Content type: Research

    Pretreatment is effective in reducing the natural recalcitrance of plant biomass so polysaccharides in cell walls can be accessed for conversion to sugars. Furthermore, lignocellulosic biomass must typically b...

    Authors: Abhishek S. Patri, Laura McAlister, Charles M. Cai, Rajeev Kumar and Charles E. Wyman

    Citation: Biotechnology for Biofuels 2019 12:177

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  9. Content type: Research

    Many studies have been carried out on the growth-modulating efficiency of plants by the colonization of an endophytic fungus Piriformospora indica. However, studies involving the co-culture of alga with endophyti...

    Authors: Vipul Swarup Bhatnagar, Prasun Bandyopadhyay, Girish H. Rajacharya, Sharanya Sarkar, Krishna Mohan Poluri and Shashi Kumar

    Citation: Biotechnology for Biofuels 2019 12:176

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  10. Content type: Research

    Valorization of lignin has the potential to significantly improve the economics of lignocellulosic biorefineries. However, its complex structure makes conversion to useful products elusive. One promising appro...

    Authors: Meghan C. Barnhart-Dailey, Dongmei Ye, Dulce C. Hayes, Danae Maes, Casey T. Simoes, Leah Appelhans, Amanda Carroll-Portillo, Michael S. Kent and Jerilyn A. Timlin

    Citation: Biotechnology for Biofuels 2019 12:175

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  11. Content type: Research

    The production of glucose from cellulose requires cellulases, which are obtained from decomposing microorganisms such as fungi and bacteria. Among the cellulases, β-glucosidases convert cellobiose to glucose a...

    Authors: Raíza Azevedo, Jéssika Lawall Lopes, Manuel Macedo de Souza, Betania Ferraz Quirino, Letícia Jungmann Cançado and Luis Fernando Marins

    Citation: Biotechnology for Biofuels 2019 12:174

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  12. Content type: Research

    Isobutanol is a promising candidate as second-generation biofuel and has several advantages compared to bioethanol. Another benefit of isobutanol is that it is already formed as a by-product in fermentations w...

    Authors: Johannes Wess, Martin Brinek and Eckhard Boles

    Citation: Biotechnology for Biofuels 2019 12:173

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  13. Content type: Research

    Plant seed oil is an important bioresource for human food and animal feed, as well as industrial bioproducts. Therefore, increasing oil content in seeds has been one of the primary targets in the breeding prog...

    Authors: Xi Xie, Dauenpen Meesapyodsuk and Xiao Qiu

    Citation: Biotechnology for Biofuels 2019 12:172

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  14. Content type: Research

    Low-temperature swelling of cotton linter cellulose and subsequent gelatinization in trifluoroacetic acid (TFA) greatly enhance rates of enzymatic digestion or maleic acid–AlCl3 catalyzed conversion to hydroxymet...

    Authors: Haibing Yang, Ximing Zhang, Hao Luo, Baoyuan Liu, Tânia M. Shiga, Xu Li, Jeong Im Kim, Peter Rubinelli, Jonathan C. Overton, Varun Subramanyam, Bruce R. Cooper, Huaping Mo, Mahdi M. Abu-Omar, Clint Chapple, Bryon S. Donohoe, Lee Makowski…

    Citation: Biotechnology for Biofuels 2019 12:171

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  15. Content type: Research

    Co-pyrolysis of wastes with other feedstock can synergistically improve the rate of biomass decomposition and also help to resolve the issues related to limited availability feedstock. In this regards, synergi...

    Authors: Arun K. Vuppaladadiyam, Hao Liu, Ming Zhao, Abdul F. Soomro, Muhammad Zaki Memon and Valerie Dupont

    Citation: Biotechnology for Biofuels 2019 12:170

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  16. Content type: Methodology

    Lipid extraction for quantification of fat content in oleaginous yeasts often requires strong acids and harmful organic solvents; it is laborious and time-consuming. Therefore, in most cases just endpoint meas...

    Authors: Mikołaj Chmielarz, Sabine Sampels, Johanna Blomqvist, Jule Brandenburg, Frida Wende, Mats Sandgren and Volkmar Passoth

    Citation: Biotechnology for Biofuels 2019 12:169

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  17. Content type: Research

    In industrial oleaginous microalgae such as Nannochloropsis spp., the key components of the carbon concentration mechanism (CCM) machineries are poorly defined, and how they are mobilized to facilitate cellular u...

    Authors: Li Wei, Mohamed El Hajjami, Chen Shen, Wuxin You, Yandu Lu, Jing Li, Xiaoyan Jing, Qiang Hu, Wenxu Zhou, Ansgar Poetsch and Jian Xu

    Citation: Biotechnology for Biofuels 2019 12:168

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  18. Content type: Review

    After just more than 100 years of history of industrial acetone–butanol–ethanol (ABE) fermentation, patented by Weizmann in the UK in 1915, butanol is again today considered a promising biofuel alternative bas...

    Authors: Cansu Birgen, Peter Dürre, Heinz A. Preisig and Alexander Wentzel

    Citation: Biotechnology for Biofuels 2019 12:167

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  19. Content type: Research

    H2–ethanol-coproducing bacteria, as primary fermenters, play important roles in the microbiome of bioreactors for bioenergy production from organic wastewater or solid wastes. Ethanoligenens harbinense YUAN-3 is ...

    Authors: Huahua Li, Xiaoxue Mei, Bingfeng Liu, Guojun Xie, Nanqi Ren and Defeng Xing

    Citation: Biotechnology for Biofuels 2019 12:166

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  20. Content type: Research

    We have recently developed a one-step, genetically encoded immobilization approach based on fusion of a target enzyme to the self-crystallizing protein Cry3Aa, followed by direct production and isolation of th...

    Authors: Bradley S. Heater, Wai Shan Chan, Marianne M. Lee and Michael K. Chan

    Citation: Biotechnology for Biofuels 2019 12:165

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  21. Content type: Research

    Escherichia coli has been proved to be one promising platform chassis for the production of various natural products, such as biofuels. Product toxicity is one of the main bottlenecks for achieving maximum produc...

    Authors: Guochao Xu, Anning Wu, Lin Xiao, Ruizhi Han and Ye Ni

    Citation: Biotechnology for Biofuels 2019 12:164

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  22. Content type: Research

    Efficient and economically viable production of biofuels from lignocellulosic biomass is dependent on mechanical and chemical pretreatment and enzymatic hydrolysis of plant material. These processing steps yie...

    Authors: Kyle R. Pomraning, James R. Collett, Joonhoon Kim, Ellen A. Panisko, David E. Culley, Ziyu Dai, Shuang Deng, Beth A. Hofstad, Mark G. Butcher and Jon K. Magnuson

    Citation: Biotechnology for Biofuels 2019 12:162

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  23. Content type: Research

    The increasing interest in replacing petroleum-based products by more sustainable materials in the packaging sector gives relevance to cellulose as a biodegradable natural resource. Moreover, its properties ca...

    Authors: Cristina Valls, F. I. Javier Pastor, M. Blanca Roncero, Teresa Vidal, Pilar Diaz, Josefina Martínez and Susana V. Valenzuela

    Citation: Biotechnology for Biofuels 2019 12:161

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  24. Content type: Research

    Antibiotics and antibiotic resistance genes (ARGs) are two pollutants in soil, especially ARGs as one of the top three threats to human health. The performance of soil microbial fuel cells (MFCs) fuelled with ...

    Authors: Xiaodong Zhao, Xiaojing Li, Yue Li, Yang Sun, Xiaolin Zhang, Liping Weng, Tianzhi Ren and Yongtao Li

    Citation: Biotechnology for Biofuels 2019 12:160

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  25. Content type: Research

    Wheat straw, the most abundant lignocellulosic biomass in China, is rich in cellulose that can be hydrolyzed and then converted into biofuels, such as bioethanol and biohydrogen. However, the accessibility of ...

    Authors: Zhichao Deng, Ao Xia, Qiang Liao, Xianqing Zhu, Yun Huang and Qian Fu

    Citation: Biotechnology for Biofuels 2019 12:159

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  26. Content type: Research

    With the increasing demand for vegetative oil and the approach of peak seed oil production, it is important to develop new oil production platforms from non-seed tissues. Castor bean (Ricinus communis) is one of ...

    Authors: Xia Wan, Qing Liu, Bei Dong, Sapna Vibhakaran Pillai, Feng-Hong Huang, Surinder P. Singh and Xue-Rong Zhou

    Citation: Biotechnology for Biofuels 2019 12:158

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  27. Content type: Research

    In the enzymatic conversion of biomass, it becomes an important issue to efficiently and cost-effectively degrade cellulose into fermentable glucose. β-Glucosidase (Bgluc), an essential member of cellulases, p...

    Authors: Yang Zhou, Xiaofeng Li, Dandan Yan, Frank Addai Peprah, Xingqi Ji, Emmanuella Esi Fletcher, Yanwei Wang, Yingying Wang, Jie Gu, Feng Lin and Haifeng Shi

    Citation: Biotechnology for Biofuels 2019 12:157

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  28. Content type: Research

    Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that cleave polysaccharides through an oxidative mechanism. These enzymes are major contributors to the recycling of carbon in nature an...

    Authors: Céline Moreau, Sandra Tapin-Lingua, Sacha Grisel, Isabelle Gimbert, Sophie Le Gall, Valérie Meyer, Michel Petit-Conil, Jean-Guy Berrin, Bernard Cathala and Ana Villares

    Citation: Biotechnology for Biofuels 2019 12:156

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  29. Content type: Review

    Microbial communities are ubiquitous in nature and exhibit several attractive features, such as sophisticated metabolic capabilities and strong environment robustness. Inspired by the advantages of natural mic...

    Authors: Yujia Jiang, Ruofan Wu, Jie Zhou, Aiyong He, Jiaxing Xu, Fengxue Xin, Wenming Zhang, Jiangfeng Ma, Min Jiang and Weiliang Dong

    Citation: Biotechnology for Biofuels 2019 12:155

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  30. Content type: Research

    In the context of sustainable development, yeast are one class of microorganisms foreseen for the production of oil from diverse renewable feedstocks, in particular those that do not compete with the food supp...

    Authors: Stéphane Thomas, Daniel R. A. Sanya, Florian Fouchard, Huu-Vang Nguyen, Gotthard Kunze, Cécile Neuvéglise and Anne-Marie Crutz-Le Coq

    Citation: Biotechnology for Biofuels 2019 12:154

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  31. Content type: Correction

    The original version of the article [1] unfortunately contained a mistake in author’s second name. The name of the author has been corrected from Thomas Breuck to Thomas Brück in this correction article.

    Authors: Boutheina Bessadok, Andrea Santulli, Thomas Brück and Saloua Sadok

    Citation: Biotechnology for Biofuels 2019 12:152

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    The original article was published in Biotechnology for Biofuels 2019 12:129

  32. Content type: Research

    During inevitable light/dark cycle, lipid productivity of outdoor microalgae photoautotrophic cultivation is lowered by nightly biomass and lipid loss. To minimize, or even reverse the nightly lipid loss, it w...

    Authors: Feng Qi, Haiyan Pei, Ruimin Mu, Guixia Ma, Daoji Wu and Qiang Han

    Citation: Biotechnology for Biofuels 2019 12:151

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  33. Content type: Research

    3-Hydroxypropionic acid (3-HP) is a promising platform chemical with various industrial applications. Several metabolic routes to produce 3-HP from organic substrates such as sugars or glycerol have been imple...

    Authors: Christian Arenas-López, Jessica Locker, Diego Orol, Frederik Walter, Tobias Busche, Jörn Kalinowski, Nigel P. Minton, Katalin Kovács and Klaus Winzer

    Citation: Biotechnology for Biofuels 2019 12:150

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  34. Content type: Methodology

    Fungal fermentation is used to produce a diverse repertoire of enzymes, chemicals, and drugs for various industries. During submerged cultivation, filamentous fungi form a range of macromorphologies, including...

    Authors: Timothy C. Cairns, Claudia Feurstein, Xiaomei Zheng, Ping Zheng, Jibin Sun and Vera Meyer

    Citation: Biotechnology for Biofuels 2019 12:149

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  35. Content type: Research

    Microbial lipids derived from various lignocellulosic feedstocks have emerged as a promising candidate for the biodiesel industry and a potential substitute for high value-added fats. However, lignocellulosic ...

    Authors: Wenting Zhou, Mou Tang, Tao Zou, Na Peng, Mi Zhao and Zhiwei Gong

    Citation: Biotechnology for Biofuels 2019 12:148

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  36. Content type: Research

    Methane is the primary component of natural gas and biogas. The huge abundance of methane makes it a promising alternative carbon source for industrial biotechnology. Herein, we report diamine compound, putres...

    Authors: Linh Thanh Nguyen and Eun Yeol Lee

    Citation: Biotechnology for Biofuels 2019 12:147

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  37. Content type: Research

    Trichoderma reesei is the most important industrial producer of lignocellulolytic enzymes. These enzymes play an important role in biomass degradation leading to novel applications of this fungus in the biotechno...

    Authors: Renato Graciano de Paula, Amanda Cristina Campos Antoniêto, Karoline Maria Vieira Nogueira, Liliane Fraga Costa Ribeiro, Marina Campos Rocha, Iran Malavazi, Fausto Almeida and Roberto Nascimento Silva

    Citation: Biotechnology for Biofuels 2019 12:146

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  38. Content type: Research

    Bacillus amyloliquefaciens NB is a newly discovered strain, which produces poly-(γ-glutamic acid) (γ-PGA) from raw extracted inulin of Jerusalem artichoke tubers; however, the underlying mechanisms remain unknown...

    Authors: Yibin Qiu, Yifan Zhu, Yijing Zhan, Yatao Zhang, Yuanyuan Sha, Yijing Zhan, Zongqi Xu, Sha Li, Xiaohai Feng and Hong Xu

    Citation: Biotechnology for Biofuels 2019 12:145

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  39. Content type: Research

    The α-l-arabinofuranosidases (α-l-ABFs) are exoenzymes involved in the hydrolysis of α-l-arabinosyl linkages in plant cell wall polysaccharides. They play a crucial role in the degradation of arabinoxylan and ara...

    Authors: Mohamed Mroueh, Marion Aruanno, Romain Borne, Pascale de Philip, Henri-Pierre Fierobe, Chantal Tardif and Sandrine Pagès

    Citation: Biotechnology for Biofuels 2019 12:144

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  40. Content type: Research

    The extremely thermophilic bacterium Caldicellulosiruptor lactoaceticus can degrade and metabolize untreated lignocellulosic biomass containing xylan. The mechanism of the bacterium for degradation of insoluble x...

    Authors: Xiaojing Jia and Yejun Han

    Citation: Biotechnology for Biofuels 2019 12:143

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  41. Content type: Correction

    After the publication of the article [1], it was brought to our attention that the GenBank Accession number for the EF1 is missing in Methods section. Please find the accession number in the erratum below.

    Authors: Wagner Rodrigo de Souza, Thályta Fraga Pacheco, Karoline Estefani Duarte, Bruno Leite Sampaio, Patrícia Abrão de Oliveira Molinari, Polyana Kelly Martins, Thaís Ribeiro Santiago, Eduardo Fernandes Formighieri, Felipe Vinecky, Ana Paula Ribeiro, Bárbara Andrade Dias Brito da Cunha, Adilson Kenji Kobayashi, Rowan Andrew Craig Mitchell, Dasciana de Sousa Rodrigues Gambetta and Hugo Bruno Correa Molinari

    Citation: Biotechnology for Biofuels 2019 12:142

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    The original article was published in Biotechnology for Biofuels 2019 12:111

  42. Content type: Research

    Docosahexaenoic acid (DHA, C22:6) and odd-chain fatty acids (OCFAs, C15:0 and C17:0) have attracted great interest, since they have been widely used in food and therapeutic industries, as well as chemical indu...

    Authors: Fangzhong Wang, Yali Bi, Jinjin Diao, Mingming Lv, Jinyu Cui, Lei Chen and Weiwen Zhang

    Citation: Biotechnology for Biofuels 2019 12:141

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  43. Content type: Research

    Oleaginous yeasts are considered as a potential lipid source for food, feed and biofuel production. In order to make the yeast-based lipid production environmentally and economically sustainable, there is a ne...

    Authors: Volha Shapaval, Jule Brandenburg, Johanna Blomqvist, Valeria Tafintseva, Volkmar Passoth, Mats Sandgren and Achim Kohler

    Citation: Biotechnology for Biofuels 2019 12:140

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  44. Content type: Research

    Expression of d-xylose isomerase having high catalytic activity in Saccharomyces cerevisiae (S. cerevisiae) is a prerequisite for efficient and economical production of bioethanol from cellulosic biomass. Althoug...

    Authors: Taisuke Seike, Yosuke Kobayashi, Takehiko Sahara, Satoru Ohgiya, Yoichi Kamagata and Kazuhiro E. Fujimori

    Citation: Biotechnology for Biofuels 2019 12:139

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  45. Content type: Research

    The lignocellulosic biomass feedstocks such as empty fruit bunches (EFBs) prove to be potential renewable resources owing to their abundance, low prices, and high carbohydrate contents. Generally, the conversi...

    Authors: Jae Kyun Kim, Jungwoo Yang, So Young Park, Ju-Hyun Yu and Kyoung Heon Kim

    Citation: Biotechnology for Biofuels 2019 12:138

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  46. Content type: Research

    Rhodotorula toruloides is a promising platform organism for production of lipids from lignocellulosic substrates. Little is known about the metabolic aspects of lipid production from the lignocellolosic sugar xyl...

    Authors: Ievgeniia A. Tiukova, Jule Brandenburg, Johanna Blomqvist, Sabine Sampels, Nils Mikkelsen, Morten Skaugen, Magnus Ø. Arntzen, Jens Nielsen, Mats Sandgren and Eduard J. Kerkhoven

    Citation: Biotechnology for Biofuels 2019 12:137

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  47. Content type: Research

    The biodegradation of chitin is an important part of the carbon and nitrogen cycles in nature. Speeding up the biotransformation of chitin substrates can not only reduce pollution, but also produce high value-...

    Authors: Xiaomeng Sun, Yingjie Li, Zhennan Tian, Yuanchao Qian, Huaiqiang Zhang and Lushan Wang

    Citation: Biotechnology for Biofuels 2019 12:136

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  48. Content type: Research

    Salix spp. are high-productivity crops potentially used for lignocellulosic biofuels such as bioethanol. In general, pretreatment is needed to facilitate the enzymatic depolymerization process. Biomass resistance...

    Authors: Jonas A. Ohlsson, Henrik R. Hallingbäck, Mohamed Jebrane, Anne E. Harman-Ware, Todd Shollenberger, Stephen R. Decker, Mats Sandgren and Ann-Christin Rönnberg-Wästljung

    Citation: Biotechnology for Biofuels 2019 12:135

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