The acid buffering capacity of high free ash in waste wheat straw (WWS) has been revealed to be a significant hindrance on the efficiency of autohydrolysis pretreatment. Previous researches have mainly relied ...
Authors: Wei Tang, Xinxing Wu, Chen Huang, Caoxing Huang, Chenhuan Lai and Qiang Yong
Microalgae are efficient producers of lipid-rich biomass, making them a key component in developing a sustainable energy source, and an alternative to fossil fuels. Chlorella species are of special interest becau...
Authors: Luca Dall’Osto, Stefano Cazzaniga, Zeno Guardini, Simone Barera, Manuel Benedetti, Giuseppe Mannino, Massimo E. Maffei and Roberto Bassi
Myceliophthora thermophila is a thermophilic ascomycete fungus that is used as a producer of enzyme cocktails used in plant biomass saccharification. Further development of this species as an industrial enzyme fa...
Authors: Ana Carolina dos Santos Gomes, Daniel Falkoski, Evy Battaglia, Mao Peng, Maira Nicolau de Almeida, Nancy Coconi Linares, Jean-Paul Meijnen, Jaap Visser and Ronald P. de Vries
Butanol production by fermentation has recently attracted increasingly more attention because of its mild reaction conditions and environmentally friendly properties. However, traditional feedstocks, such as c...
Authors: Zhangnan Lin, Hongjuan Liu, Jing Wu, Petra Patakova, Barbora Branska and Jianan Zhang
Microalgae as a viable biodiesel feedstock show great potential to approach the challenges of energy shortage and environment pollution, but their economic feasibility was seriously hampered by high production...
Authors: Lin Luo, Hongyu Ren, Xuanyuan Pei, Guojun Xie, Defeng Xing, Yingqi Dai, Nanqi Ren and Bingfeng Liu
5-Hydroxymethylfurfural (HMF) is a highly valuable platform chemical that can be obtained from plant biomass carbohydrates. HMF can be oxidized to 2,5-furandicarboxylic acid (FDCA), which is used as a renewabl...
Authors: Ana Serrano, Eva Calviño, Juan Carro, María I. Sánchez-Ruiz, F. Javier Cañada and Angel T. Martínez
Increasing seed oil content is one of the most important targets for rapeseed (Brassica napus) breeding. However, genetic mechanisms of mature seed oil content in Brassica napus (B. napus) remain little known. To...
Authors: Zhongchun Xiao, Chao Zhang, Fang Tang, Bo Yang, Liyuan Zhang, Jingsen Liu, Qiang Huo, Shufeng Wang, Shengting Li, Lijuan Wei, Hai Du, Cunmin Qu, Kun Lu, Jiana Li and Nannan Li
Animal bedding remains an underutilized source of raw material for bioethanol production, despite the economic and environmental benefits of its use. Further research concerning the optimization of the product...
Authors: Miguel Sanchis-Sebastiá, Borbála Erdei, Krisztina Kovacs, Mats Galbe and Ola Wallberg
As a renewable and clean energy carrier, the production of biohydrogen from low-value feedstock such as lignocellulose has increasingly garnered interest. The NADH-dependent reduced ferredoxin:NADP+ oxidoreductas...
Authors: Yang Li, Jialei Hu, Chunyun Qu, Lili Chen, Xiaolong Guo, Hongxin Fu and Jufang Wang
In this work, three pretreatments under investigation at the DOE Bioenergy Research Centers (BRCs) were subjected to a side-by-side comparison to assess their performance on model bioenergy hardwoods (a eucaly...
Authors: Aditya Bhalla, Charles M. Cai, Feng Xu, Sandip K. Singh, Namita Bansal, Thanaphong Phongpreecha, Tanmoy Dutta, Cliff E. Foster, Rajeev Kumar, Blake A. Simmons, Seema Singh, Charles E. Wyman, Eric L. Hegg and David B. Hodge
Lacto-N-neotetraose (LNnT) is one of the important ingredients of human milk oligosaccharides, which can enhance immunity, regulate intestinal bacteria and promote cell maturation.
Authors: Xiaomin Dong, Nan Li, Zhenmin Liu, Xueqin Lv, Jianghua Li, Guocheng Du, Miao Wang and Long Liu
S-Adenosylmethionine (SAM) is a critical cofactor involved in many biochemical reactions. However, the low fermentation titer of SAM in methionine-free medium hampers commercial-scale production. The SAM synthe.....
Authors: Liying Ruan, Lu Li, Dian Zou, Cong Jiang, Zhiyou Wen, Shouwen Chen, Yu Deng and Xuetuan Wei
Biofuels derived from lignocellulosic biomass are a viable alternative to fossil fuels required for transportation. Following plant biomass pretreatment, the furan derivative furfural is present at concentrati...
Authors: Daria Feldman, David J. Kowbel, Adi Cohen, N. Louise Glass, Yitzhak Hadar and Oded Yarden
Schizochytrium sp. is a marine fungus with great potential as an alternative commercial source of lipids rich in polyunsaturated fatty acids (PUFAs). To further increase lipid accumulation in Schizochytrium sp., ...
Authors: Zhipeng Li, Xueping Ling, Hao Zhou, Tong Meng, Jinjin Zeng, Wei Hang, Yanyan Shi and Ning He
In anaerobic cellulolytic micro-organisms, cellulolysis results in the action of several cellulases gathered in extracellular multi-enzyme complexes called cellulosomes. Their action releases cellobiose and lo...
Authors: Nian Liu, Aurélie Fosses, Clara Kampik, Goetz Parsiegla, Yann Denis, Nicolas Vita, Henri-Pierre Fierobe and Stéphanie Perret
Formate converted from CO2 reduction has great potential as a sustainable feedstock for biological production of biofuels and biochemicals. Nevertheless, utilization of formate for growth and chemical production ...
Authors: Seung-Jin Kim, Jihee Yoon, Dae-Kyun Im, Yong Hwan Kim and Min-Kyu Oh
Cellulose-active lytic polysaccharide monooxygenases (LPMOs) secreted by filamentous fungi play a key role in the degradation of recalcitrant lignocellulosic biomass. They can occur as multidomain proteins fus...
Authors: Amani Chalak, Ana Villares, Celine Moreau, Mireille Haon, Sacha Grisel, Angélina d’Orlando, Isabelle Herpoël-Gimbert, Aurore Labourel, Bernard Cathala and Jean-Guy Berrin
2,3-Butanediol (2,3-BDO) is a valuable chemical for industrial applications. Bacteria can produce 2,3-BDO with a high productivity, though most of their classification as pathogens makes them undesirable for t...
Compared to the oleaginous yeast Yarrowia lipolytica, Trichosporon cutaneum can metabolize pentose sugars more efficiently, and in the meantime is more tolerant to inhibitors, which is suitable for lipid producti...
Authors: Ya Wang, Riming Yan, Lijuan Tang, Libin Zhu, Du Zhu and Fengwu Bai
Oleaginous yeasts are potent hosts for the renewable production of lipids and harbor great potential for derived products, such as biofuels. Several promising processes have been described that produce hydroca...
Authors: Stefan Bruder, Eva Johanna Moldenhauer, Robert Denis Lemke, Rodrigo Ledesma-Amaro and Johannes Kabisch
Consolidated bioprocessing (CBP) combines enzyme production, saccharification and fermentation into a one-step process. This strategy represents a promising alternative for economic ethanol production from sta...
Authors: Rosemary A. Cripwell, Shaunita H. Rose, Lorenzo Favaro and Willem H. van Zyl
Filamentous fungi secret hydrolytic enzymes like cellulase and hemicellulase outside the cells, serving as important scavengers of plant biomass in nature and workhorses in the enzyme industry. Unlike the exte...
Authors: Chengcheng Li, Ai-Ping Pang, Hang Yang, Roujing Lv, Zhihua Zhou, Fu-Gen Wu and Fengming Lin
A consolidated bioprocessing (CBP), where lignocellulose is converted into the desired product(s) in a single fermentative step without the addition of expensive degradative enzymes, represents the ideal solut...
Authors: Zeenat Bashir, Lili Sheng, Annamma Anil, Arvind Lali, Nigel P. Minton and Ying Zhang
Enzymatic hydrolysis of lignocellulose by fungi is a key step in global carbon cycle and biomass utilization. Cellulolytic enzyme production is tightly controlled at a transcriptional level. Here, we investiga...
Layered double hydroxides (LDHs) have received widespread attention for their potential applications in catalysis, polymer nanocomposites, pharmaceuticals, and sensors. Here, the mechanism underlying the physi...
Authors: Wei Ren, Yanshuai Ding, Lide Gu, Wanli Yan, Cang Wang, Mingsheng Lyu, Changhai Wang and Shujun Wang
Cellulolytic enzymes produced by Trichoderma reesei are widely used for the industrial production of biofuels and chemicals from lignocellulose. We speculated that intracellular pH during the fermentation process...
Authors: Pei Liu, Guoxiu Zhang, Yumeng Chen, Jian Zhao, Wei Wang and Dongzhi Wei
Black soldier fly (BSF, Hermetia illucens L.) can efficiently degrade organic wastes and transform into a high fat containing insect biomass that could be used as feedstock for biodiesel production. Meanwhile, th...
Authors: Zhaolu Zhu, Kashif ur Rehman, Yongqiang Yu, Xiu Liu, Hui Wang, Jeffery K. Tomberlin, Sing-Hoi Sze, Minmin Cai, Jibin Zhang, Ziniu Yu, Jinshui Zheng and Longyu Zheng
Switchgrass (Panicum virgatum L.), a warm-season perennial C4 plant, can be used as a forage plant, a soil and water conservation plant, a windbreak plant, and as a good source of biofuels and alternative energy ...
Authors: Shumeng Zhang, Fengli Sun, Weiwei Wang, Guoyu Yang, Chao Zhang, Yongfeng Wang, Shudong Liu and Yajun Xi
The current extraction and use of fossil fuels has been linked to extensive negative health and environmental outcomes. Lignocellulosic biomass-derived biofuels and bioproducts are being actively considered as...
Authors: Winston E. Anthony, Rhiannon R. Carr, Drew M. DeLorenzo, Tayte P. Campbell, Zeyu Shang, Marcus Foston, Tae Seok Moon and Gautam Dantas
Microalgae are one of the promising feedstock that consists of high carbohydrate content which can be converted into bioethanol. Pre-treatment is one of the critical steps required to release fermentable sugar...
Authors: Chai Kee Phwan, Kit Wayne Chew, Abdi Hanra Sebayang, Hwai Chyuan Ong, Tau Chuan Ling, Marlinda Abdul Malek, Yeek-Chia Ho and Pau Loke Show
Xylan is the major component of hemicelluloses, which are the second most abundant polysaccharides in nature, accounting for approximately one-third of all renewable organic carbon resources on earth. Efficien...
Authors: Xueqiang Liu, Zhengqiang Jiang, Yu Liu, Xin You, Shaoqing Yang and Qiaojuan Yan
Prehydrolyzate, which is from the prehydrolysis process in dissolving pulps industry, contains various sugar-derived and lignin compounds such as xylooligosaccharides (XOS), gluco-oligosaccharides, xylose, glu...
Authors: Caoxing Huang, Xucai Wang, Chen Liang, Xiao Jiang, Gan Yang, Jie Xu and Qiang Yong
Biorefineries are widely recognized as the most feasible solution to the problem of achieving environmental sustainability along with economic growth. Furthermore, pine wilt disease has caused severe environme...
Authors: Ho Myeong Kim, In Seong Choi, Seoyoun Lee, In Min Hwang, Ho Hyun Chun, Seung Gon Wi, Jin-Cheol Kim, Tae Young Shin, Jong Cheol Kim, Jae Su Kim, Junheon Kim and Hae Woong Park
Yarrowia lipolytica, an oleaginous yeast, is a promising platform strain for production of biofuels and oleochemicals as it can accumulate a high level of lipids in response to nitrogen limitation. Accordingly, m...
Authors: Minsuk Kim, Beom Gi Park, Eun-Jung Kim, Joonwon Kim and Byung-Gee Kim
Biofuel production from plant cell walls offers the potential for sustainable and economically attractive alternatives to petroleum-based products. In particular, Clostridium thermocellum is a promising host for ...
Authors: Liang Tian, Peter M. Conway, Nicholas D. Cervenka, Jingxuan Cui, Marybeth Maloney, Daniel G. Olson and Lee R. Lynd
Efforts to develop efficient lignocellulose-degrading enzymatic preparations have led to the relatively recent discovery of a new class of novel cellulase boosters, termed lytic polysaccharide monoxygenases (L...
Authors: Pooja Dixit, Biswajit Basu, Munish Puri, Deepak Kumar Tuli, Anshu Shankar Mathur and Colin James Barrow
Microalgal starch is regarded as a promising alternative to crop-based starch for biorefinery such as the production of biofuels and bio-based chemicals. The single or separate use of inorganic carbon source, ...
Authors: Man Qi, Changhong Yao, Binhuan Sun, Xupeng Cao, Qiang Fei, Bobo Liang, Wenyi Ran, Qi Xiang, Yongkui Zhang and Xianqiu Lan
Efficient biotechnological conversion of lignocellulosic biomass to valuable products, such as transportation biofuels, is ecologically attractive, yet requires substantially improved mechanistic understanding...
Decreasing fossil fuels and its impact on global warming have led to an increasing demand for its replacement by sustainable renewable biofuels. Microalgae may offer a potential feedstock for renewable biofuel...
Authors: Kashif Mohd Shaikh, Asha Arumugam Nesamma, Malik Zainul Abdin and Pannaga Pavan Jutur
Dedicated lignocellulosic feedstock from grass crops for biofuel production is extensively increasing. However, the access to fermentable cell wall sugars by carbohydrate degrading enzymes is impeded by lignin...
Authors: Philippe Le Bris, Yin Wang, Clément Barbereau, Sébastien Antelme, Laurent Cézard, Frédéric Legée, Angelina D’Orlando, Marion Dalmais, Abdelhafid Bendahmane, Mathias Schuetz, Lacey Samuels, Catherine Lapierre and Richard Sibout
DNA assembly is an essential technique enabling metabolic engineering and synthetic biology. Combining novel DNA assembly technologies with rational metabolic engineering can facilitate the construction of mic...
Authors: Shuwen Liu, Haihan Xiao, Fangfang Zhang, Zheng Lu, Yun Zhang, Aihua Deng, Zhongcai Li, Cui Yang and Tingyi Wen
Countercurrent saccharification is a promising way to minimize enzyme loading while obtaining high conversions and product concentrations. However, in countercurrent saccharification experiments, 3–4 months ar...
Authors: Chao Liang, Chao Gu, M. Nazmul Karim and Mark Holtzapple
Algal harvesting is a major cost which increases biofuel production cost. Algal biofuels are widely studied as third-generation biofuel. However, they are yet not viable because of its high production cost whi...
Authors: Arghya Bhattacharya, Megha Mathur, Pushpendar Kumar and Anushree Malik
Pretreatment is effective in reducing the natural recalcitrance of plant biomass so polysaccharides in cell walls can be accessed for conversion to sugars. Furthermore, lignocellulosic biomass must typically b...
Authors: Abhishek S. Patri, Laura McAlister, Charles M. Cai, Rajeev Kumar and Charles E. Wyman
Many studies have been carried out on the growth-modulating efficiency of plants by the colonization of an endophytic fungus Piriformospora indica. However, studies involving the co-culture of alga with endophyti...
Authors: Vipul Swarup Bhatnagar, Prasun Bandyopadhyay, Girish H. Rajacharya, Sharanya Sarkar, Krishna Mohan Poluri and Shashi Kumar
Valorization of lignin has the potential to significantly improve the economics of lignocellulosic biorefineries. However, its complex structure makes conversion to useful products elusive. One promising appro...
Authors: Meghan C. Barnhart-Dailey, Dongmei Ye, Dulce C. Hayes, Danae Maes, Casey T. Simoes, Leah Appelhans, Amanda Carroll-Portillo, Michael S. Kent and Jerilyn A. Timlin
The production of glucose from cellulose requires cellulases, which are obtained from decomposing microorganisms such as fungi and bacteria. Among the cellulases, β-glucosidases convert cellobiose to glucose a...
Authors: Raíza Azevedo, Jéssika Lawall Lopes, Manuel Macedo de Souza, Betania Ferraz Quirino, Letícia Jungmann Cançado and Luis Fernando Marins
Isobutanol is a promising candidate as second-generation biofuel and has several advantages compared to bioethanol. Another benefit of isobutanol is that it is already formed as a by-product in fermentations w...
Authors: Johannes Wess, Martin Brinek and Eckhard Boles
Plant seed oil is an important bioresource for human food and animal feed, as well as industrial bioproducts. Therefore, increasing oil content in seeds has been one of the primary targets in the breeding prog...
Authors: Xi Xie, Dauenpen Meesapyodsuk and Xiao Qiu
Lipid extraction for quantification of fat content in oleaginous yeasts often requires strong acids and harmful organic solvents; it is laborious and time-consuming. Therefore, in most cases just endpoint meas...
Authors: Mikołaj Chmielarz, Sabine Sampels, Johanna Blomqvist, Jule Brandenburg, Frida Wende, Mats Sandgren and Volkmar Passoth
