The co-production of single cell oil (SCO) with value-added products could improve the economic viability of industrial SCO production. The newly isolated oleaginous yeast Cryptococcus podzolicus DSM 27192 was ab...
Authors: Xiujuan Qian, Olga Gorte, Lin Chen, Wenming Zhang, Weiliang Dong, Jiangfeng Ma, Min Jiang, Fengxue Xin and Katrin Ochsenreither
Fast, complete, and ultimate removal of inhibitory compounds derived from lignocellulose pretreatment is the prerequisite for efficient production of cellulosic ethanol and biochemicals. Biodetoxification is t...
Authors: Xia Yi, Qiuqiang Gao, Lei Zhang, Xia Wang, Yanqing He, Fengxian Hu, Jian Zhang, Gen Zou, Shihui Yang, Zhihua Zhou and Jie Bao
Microalgae have been experimented as a potential feedstock for biofuel generation in current era owing to its’ rich energy content, inflated growth rate, inexpensive culture approaches, the notable capacity of CO
Authors: Nazia Hossain, T. M. I. Mahlia and R. Saidur
Reducing fresh water consumption and nutrient addition will be an effective way to reduce the whole cost of bioethanol production. On the other hand, treatment of biogas slurry derived from anaerobic digestion...
Authors: Guowei Duan, Bo Wu, Han Qin, Weiting Wang, Qiong Tan, Yonghua Dai, Yao Qin, Furong Tan, Guoquan Hu and Mingxiong He
During current submerged fermentation for microbial lipid production, the large-scale reactor operations inevitably consume substantial amounts of water and electricity for aeration, stirring, and temperature ...
Authors: Lei Liu, Jie Song, Yi Li, Ping Li and Hailei Wang
Chlorophylls play important roles in photosynthesis, and thus are critical for growth and related metabolic pathways in photosynthetic organisms. They are particularly important in microalgae, emerging as the ...
Authors: Hyun Gi Koh, Nam Kyu Kang, Seungjib Jeon, Sung-Eun Shin, Byeong-ryool Jeong and Yong Keun Chang
Phosphorus (P) is an essential element of microalgae, which is either required for anabolism or for energy metabolism. When employing a nitrogen limitation strategy to trigger microalgal intracellular lipid ac...
Authors: Liang Fu, Qingcheng Li, Ge Yan, Dandan Zhou and John C. Crittenden
The main representatives of hemicellulose are xylans, usually decorated β-1,4-linked d-xylose polymers, which are hydrolyzed by xylanases. The efficient utilization and complete hydrolysis of xylans necessitate t...
Authors: Constantinos Katsimpouras, Grigorios Dedes, Nikolaos S. Thomaidis and Evangelos Topakas
Microalgae are attracting much attention as a promising feedstock for renewable energy production, while simultaneously providing environmental benefits. So far, comparison studies for microalgae selection for...
Authors: A. Jebali, F. G. Acién, N. Jiménez-Ruiz, C. Gómez, J. M. Fernández-Sevilla, N. Mhiri, F. Karray, S. Sayadi and E. Molina-Grima
The glucose–methanol–choline (GMC) superfamily is a large and functionally diverse family of oxidoreductases that share a common structural fold. Fungal members of this superfamily that are characterised and r...
Authors: Leander Sützl, Gabriel Foley, Elizabeth M J Gillam, Mikael Bodén and Dietmar Haltrich
Lytic polysaccharide monooxygenases (LPMOs) opened a new horizon for biomass deconstruction. They use a redox mechanism not yet fully understood and the range of substrates initially envisaged to be the crysta...
Authors: Thamy Lívia Ribeiro Corrêa, Atílio Tomazini Júnior, Lúcia Daniela Wolf, Marcos Silveira Buckeridge, Leandro Vieira dos Santos and Mario Tyago Murakami
Yeast strains that are tolerant to multiple environmental stresses are highly desired for various industrial applications. Despite great efforts in identifying key genes involved in stress tolerance of budding...
Authors: Ming-Ming Zhang, Liang Xiong, Ya-Jie Tang, Muhammad Aamer Mehmood, Zongbao Kent Zhao, Feng-Wu Bai and Xin-Qing Zhao
(Pseudo)Bacteroides cellulosolvens is a cellulolytic bacterium that produces the most extensive and intricate cellulosomal system known in nature. Recently, the elaborate architecture of the B. cellulosolvens cel...
Authors: Olga Zhivin-Nissan, Bareket Dassa, Ely Morag, Meital Kupervaser, Yishai Levin and Edward A. Bayer
In the present work, the main inhibitors of the yeast cells (vanillin, furfural, formic, and levulinic acid) were generated by pretreatments or hydrolysis (sulfuric acid or enzymes) to convert reducing sugars ...
Authors: Cecilia Laluce, Longinus I. Igbojionu, José L. Silva and Clóvis A. Ribeiro
With the increased attention on bio-based industry, demands for techniques that enable fast and effective strain improvement have been dramatically increased. Evolutionary engineering, which is less dependent ...
Authors: Jina Yang, Beomhee Kim, Gi Yeon Kim, Gyoo Yeol Jung and Sang Woo Seo
Clostridium spp. can synthesize valuable chemicals and fuels by utilizing diverse waste-stream substrates, including starchy biomass, lignocellulose, and industrial waste gases. However, metabolic engineering in
Authors: Gabriele Philipps, Sebastian de Vries and Stefan Jennewein
Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal c...
Authors: Wagner Rodrigo de Souza, Thályta Fraga Pacheco, Karoline Estefani Duarte, Bruno Leite Sampaio, Patrícia Abrão de Oliveira Molinari, Polyana Kelly Martins, Thaís Ribeiro Santiago, Eduardo Fernandes Formighieri, Felipe Vinecky, Ana Paula Ribeiro, Bárbara Andrade Dias Brito da Cunha, Adilson Kenji Kobayashi, Rowan Andrew Craig Mitchell, Dasciana de Sousa Rodrigues Gambetta and Hugo Bruno Correa Molinari
Lipolytic enzymes of hyperthermophilic archaea generally prefer small carbon chain fatty acid esters (C2–C12) and are categorized as esterases. However, a few have shown activity with long-chain fatty acid esters...
Authors: Surabhi Soni, Sneha S. Sathe, Rutuja R. Sheth, Prince Tiwari, Rajesh-Kumar N. Vadgama, Annamma Anil Odaneth, Arvind M. Lali and Sanjeev K. Chandrayan
Grass glucuronoarabinoxylan (GAX) substitutions can inhibit enzymatic degradation and are involved in the interaction of xylan with cell wall cellulose and lignin, factors which contribute to the recalcitrance...
Authors: Theodora Tryfona, Mathias Sorieul, Carolina Feijao, Katherine Stott, Denis V. Rubtsov, Nadine Anders and Paul Dupree
Downregulation of genes involved in lignin biosynthesis and related biochemical pathways has been used as a strategy to improve biofuel production. Plant C1 metabolism provides the methyl units used for the me...
Authors: Hongli Xie, Nancy L. Engle, Sivasankari Venketachalam, Chang Geun Yoo, Jaime Barros, Mitch Lecoultre, Nikki Howard, Guifen Li, Liang Sun, Avinash C. Srivastava, Sivakumar Pattathil, Yunqiao Pu, Michael G. Hahn, Arthur J. Ragauskas, Richard S. Nelson, Richard A. Dixon…
Due to the intact structure of lignocellulosic biomass, pretreatment was a prerequisite to improve the enzymatic hydrolysis by disrupting the recalcitrant lignocellulose and increasing the accessibility of cel...
Authors: Hongdan Zhang, Shihang Huang, Weiqi Wei, Jiajie Zhang and Jun Xie
Monitoring and providing early warning are essential operations in the anaerobic digestion (AD) process. However, there are still several challenges for identifying the early warning indicators and their thres...
Authors: Yiran Wu, Adam Kovalovszki, Jiahao Pan, Cong Lin, Hongbin Liu, Na Duan and Irini Angelidaki
Soil ascomycete fungi produce plant-biomass-degrading enzymes to facilitate nutrient and energy uptake in response to exogenous stress. This is controlled by a complex signal network, but the regulatory mechan...
Commercial biogas upgrading facilities are expensive and consume energy. Biological biogas upgrading may serve as a low-cost approach because it can be easily integrated with existing facilities at biogas plan...
Authors: Radziah Wahid, Daniel Girma Mulat, John Christian Gaby and Svein Jarle Horn
Solid-state fermentation (SSF) mimics the natural decay environment of soil fungi and can be employed to investigate the production of plant biomass-degrading enzymes. However, knowledge on the transcriptional...
Insight into the function of carbohydrate-active enzymes is required to understand their biological role and industrial potential. There is a need for better use of the ample genomic data in order to enable se...
Clostridium acetobutylicum is a model fermentative anaerobe for consolidated bioprocessing of lignocellulose hydrolysates into acetone–butanol–ethanol (ABE). However, the main inhibitors (acids, furans and phenol...
Authors: Huanhuan Liu, Jing Zhang, Jian Yuan, Xiaolong Jiang, Lingyan Jiang, Guang Zhao, Di Huang and Bin Liu
The urban sewer system is an important component of urban water infrastructure for sewage collection and transportation, and in-sewer transportation of sewage can cause multitudinous contaminant degradations w...
Authors: Pengkang Jin, Yonggang Gu, Xuan Shi and Wenna Yang
Miscanthus is a leading bioenergy crop with enormous lignocellulose production potential for biofuels and chemicals. However, lignocellulose recalcitrance leads to biomass process difficulty for an efficient bioe...
Authors: Aftab Alam, Ran Zhang, Peng Liu, Jiangfeng Huang, Yanting Wang, Zhen Hu, Meysam Madadi, Dan Sun, Ruofei Hu, Arthur J. Ragauskas, Yuanyuan Tu and Liangcai Peng
Yeast has been the focus of development of cell biofactories for the production of lipids and interest in the field has been driven by the need for sustainably sourced lipids for use in a broad range of indust...
Authors: Huadong Peng, Lizhong He and Victoria S. Haritos
Propionic acid (PA), a key platform chemical produced as a by-product during petroleum refining, has been widely used as a food preservative and an important chemical intermediate in many industries. Microbial...
Authors: Xin Xu, Thomas C. Williams, Christina Divne, Isak S. Pretorius and Ian T. Paulsen
We recently confirmed that the deactivation of T. reesei cellulases at the air–liquid interface reduces microcrystalline cellulose conversion at low enzyme loadings in shaken flasks. It is one of the main causes ...
Authors: Samarthya Bhagia, Charles E. Wyman and Rajeev Kumar
Starch is an inexpensive and renewable raw material for numerous industrial applications. However, most starch-based products are not cost-efficient due to high-energy input needed in traditional enzymatic sta...
4-Hydroxyphenylacetic acid (4HPAA) is an important building block for synthesizing drugs, agrochemicals, biochemicals, etc. 4HPAA is currently produced exclusively via petrochemical processes and the process i...
Authors: Yu-Ping Shen, Lai San Fong, Zhi-Bo Yan and Jian-Zhong Liu
Mixers are usually inserted into microalgal photobioreactors to generate vortices that can enhance light/dark cycles of algal cells and consequently enhance biomass productivity. However, existing mixer design...
Volatile fatty acids (VFAs) are produced by fermentation of various bio-sources and human wastes at minimal cost; sometimes, even sources having a prepaid processing fee were used. However, low concentrations ...
Lignocellulosic biomass is an attractive, inexpensive source of potentially fermentable sugars. However, hydrolysis of lignocellulose results in a complex mixture containing microbial inhibitors at variable co...
Authors: Anita Singh, Stacy R. Bedore, Nilesh K. Sharma, Sarah A. Lee, Mark A. Eiteman and Ellen L. Neidle
Simultaneous cofermentation of glucose and xylose mixtures would be a cost-effective solution for the conversion of cellulosic biomass to high-value products. However, most yeasts ferment glucose and xylose se...
Authors: Saet-Byeol Kim, Deok-Ho Kwon, Jae-Bum Park and Suk-Jin Ha
Cyanobacteria produce hydrocarbons corresponding to diesel fuels by means of aldehyde-deformylating oxygenase (ADO). ADO catalyzes a difficult and unusual reaction in the conversion of aldehydes to hydrocarbon...
Authors: Hisashi Kudo, Yuuki Hayashi and Munehito Arai
Butyric fermentation and a substantial loss of dry matter (DM) often occur in alfalfa silage during the rainy season, which is not conducive to subsequent biofuel production. Currently, there have been negativ...
Authors: Qinhua Liu, Junfeng Li, Jie Zhao, Jingxing Wu and Tao Shao
Populus (poplar) tree species including hybrid varieties are considered as promising biomass feedstock for biofuels and biochemicals production due to their fast growing, short vegetative cycle, and widely distri...
Authors: Peiyao Wen, Tian Zhang, Jinye Wang, Zhina Lian and Junhua Zhang
Lignocellulose biomass contains high amount of biotin and resulted in an excessive biotin condition for cellulosic glutamic acid accumulation by Corynebacterium glutamicum. Penicillin or ethambutol triggers cellu...
Lignocellulosic biomass from dedicated energy crops such as Miscanthus spp. is an important tool to combat anthropogenic climate change. However, we still do not exactly understand the sources of cell wall recalc...
Authors: Ricardo M. F. da Costa, Sivakumar Pattathil, Utku Avci, Ana Winters, Michael G. Hahn and Maurice Bosch
The steadily increasing demand on transportation fuels calls for renewable fuel replacements. This has attracted a growing amount of research to develop advanced biofuels that have similar physical, chemical, ...
Authors: Wenqin Bai, Weitao Geng, Shaojie Wang and Fuzhong Zhang
Maize brown midrib (bm) mutants associated with impaired lignin biosynthesis are a potential source for the breed of novel germplasms with improved cell wall digestibility. The spontaneous bm5 mutants had been id...
Authors: Wangdan Xiong, Zhenying Wu, Yuchen Liu, Yu Li, Kunlong Su, Zetao Bai, Siyi Guo, Zhubing Hu, Zhiming Zhang, Yan Bao, Juan Sun, Guofeng Yang and Chunxiang Fu
Trichoderma reesei is one of the major producers of enzymes for the conversion of plant biomass to sustainable fuels and chemicals. Crude plant biomass can induce the production of CAZymes in T. reesei, but there...
Authors: Tiziano Benocci, Maria Victoria Aguilar-Pontes, Roland Sándor Kun, Ronnie J. M. Lubbers, Kathleen Lail, Mei Wang, Anna Lipzen, Vivian Ng, Igor V. Grigoriev, Bernhard Seiboth, Paul Daly and Ronald P. de Vries
Seasonal variation in microalgae production is a significant challenge to developing cost-competitive algae biofuels. Summer production can be three to five times greater than winter production, which could re...
Authors: Lynn M. Wendt, Christopher Kinchin, Bradley D. Wahlen, Ryan Davis, Thomas A. Dempster and Henri Gerken
The CYP152 family member OleTJE from Jeotgalicoccus sp. ATCC 8456 has been well-known to catalyze the unusual one-step decarboxylation of free fatty acids towards the formation of terminal alkenes. Efforts to tun...
Authors: Yuanyuan Jiang, Zhong Li, Cong Wang, Yongjin J. Zhou, Huifang Xu and Shengying Li
Marine microalga Nannochloropsis is a promising source for the production of renewable and sustainable biodiesel in replacement of depleting petroleum. Other than biodiesel, Nannochloropsis is a green and potenti...
