In the biofuel industry, cellulase plays an indispensable role in hydrolyzing cellulose into fermentable glucose. Trichoderma reesei is a popular filamentous fungus with prominent ability to produce cellulase. Wh...
Authors: Fei Gao, Zhenzhen Hao, Xianhua Sun, Lina Qin, Tong Zhao, Weiquan Liu, Huiying Luo, Bin Yao and Xiaoyun Su
Protein glycosylation pathways have been identified in a variety of bacteria and are best understood in pathogens and commensals in which the glycosylation targets are cell surface proteins, such as S layers, ...
Authors: Jordan Russell, Sun-Ki Kim, Justin Duma, Harald Nothaft, Michael E. Himmel, Yannick J. Bomble, Christine M. Szymanski and Janet Westpheling
The model yeast, Saccharomyces cerevisiae, is not known to be oleaginous. However, an industrial wild-type strain, D5A, was shown to accumulate over 20% storage lipids from glucose when growth is nitrogen-limited...
Authors: Qiaoning He, Yongfu Yang, Shihui Yang, Bryon S. Donohoe, Stefanie Van Wychen, Min Zhang, Michael E. Himmel and Eric P. Knoshaug
Methanol has become an attractive substrate for biotechnological applications due to its abundance and low-price. Chemicals production from methanol could alleviate the environmental concerns, costs, and forei...
Authors: Wenming Zhang, Meng Song, Qiao Yang, Zhongxue Dai, Shangjie Zhang, Fengxue Xin, Weiliang Dong, Jiangfeng Ma and Min Jiang
Biogas production is an attractive technology for a sustainable generation of renewable energy. Although the microbial community is fundamental for such production, the process control is still limited to tech...
Authors: Anja Grohmann, Yevhen Vainshtein, Ellen Euchner, Christian Grumaz, Dieter Bryniok, Ralf Rabus and Kai Sohn
To mitigate the climate change caused by CO2 emission, the global incentive to the low-carbon alternatives as replacement of fossil fuel-derived products continuously expands the need for renewable feedstock. The...
Authors: Si-Yu Li, I-Son Ng, Po Ting Chen, Chung-Jen Chiang and Yun-Peng Chao
Lignocellulosic biomass, such as wood and straw, is an interesting feedstock for the production of fermentable sugars. However, mainly due to the presence of lignin, this type of biomass is recalcitrant to sac...
Authors: Lisanne de Vries, Ruben Vanholme, Rebecca Van Acker, Barbara De Meester, Lisa Sundin and Wout Boerjan
Lignin is a crucial molecule for terrestrial plants, as it offers structural support and permits the transport of water over long distances. The hardness of lignin reduces plant digestibility by cattle and she...
Authors: Mojdeh Faraji, Luis L. Fonseca, Luis Escamilla-Treviño, Jaime Barros-Rios, Nancy L. Engle, Zamin K. Yang, Timothy J. Tschaplinski, Richard A. Dixon and Eberhard O. Voit
Applying very high gravity (VHG) fermentation conditions to the sugarcane juice (SCJ) bioethanol industry would improve its environmental and economic sustainability without the need for major infrastructure c...
Authors: Bruno Monteiro, Pedro Ferraz, Mário Barroca, Sandra H. da Cruz, Tony Collins and Cândida Lucas
Butanol has been regarded as an important bulk chemical and advanced biofuel; however, large scaling butanol production by solventogenic Clostridium sp. is still not economically feasible due to the high cost of ...
Authors: Fengxue Xin, Wei Yan, Jie Zhou, Hao Wu, Weiliang Dong, Jiangfeng Ma, Wenming Zhang and Min Jiang
Thraustochytrids are heterotrophic, oleaginous, marine protists with a significant potential for biofuel production. High-value co-products can off-set production costs; however, the cost of raw materials, and...
Authors: Alexandra Merkx-Jacques, Holly Rasmussen, Denise M. Muise, Jeremy J. R. Benjamin, Haila Kottwitz, Kaitlyn Tanner, Michael T. Milway, Laura M. Purdue, Mark A. Scaife, Roberto E. Armenta and David L. Woodhall
Schizochytrium sp. is a promising strain for the production of docosahexaenoic acid (DHA)-rich oil and biodiesel, and has been widely used in the food additive and bioenergy industries. Oxygen is a particularly i...
Authors: Zhi-Qian Bi, Lu-Jing Ren, Xue-Chao Hu, Xiao-Man Sun, Si-Yu Zhu, Xiao-Jun Ji and He Huang
Xylan removal by bacterial pretreatments has been confirmed to increase the digestibility of biomass. Here, an effective xylan removal technique has been developed to enhance the digestibility of wheat straw a...
Authors: Haipeng Guo, Chuntao Hong, Bingsong Zheng, Dean Jiang and Wensheng Qin
Sugarcane bagasse is an abundant and geographically widespread agro-industrial residue with high carbohydrate content, making it a strong candidate feedstock for the bio-based economy. This study examines the ...
Authors: Clementine L. Chambon, Thandeka Y. Mkhize, Prashant Reddy, Agnieszka Brandt-Talbot, Nirmala Deenadayalu, Paul S. Fennell and Jason P. Hallett
Milling during fermentation, termed cotreatment, has recently been proposed as an alternative to thermochemical pretreatment as a means to increase the accessibility of lignocellulosic biomass to biological at...
Authors: Sanchari Ghosh, Evert K. Holwerda, Robert S. Worthen, Lee R. Lynd and Brenden P. Epps
MicroRNAs are a class of endogenous non-coding RNAs that play a vital role in post-transcriptional gene regulation in eukaryotic cells. In plants and animals, miRNAs are implicated in diverse roles ranging fro...
Authors: Sulin Lou, Ting Sun, Hui Li and Zhangli Hu
Biogenic and biogenic-thermogenic coalbed methane (CBM) are important energy reserves for unconventional natural gas. Thus, to investigate biogenic gas formation mechanisms, a series of fresh coal samples from...
Clostridium thermocellum has been the subject of multiple metabolic engineering strategies to improve its ability to ferment cellulose to ethanol, with varying degrees of success. For ethanol production in C. the...
Authors: Shuen Hon, Evert K. Holwerda, Robert S. Worthen, Marybeth I. Maloney, Liang Tian, Jingxuan Cui, Paul P. Lin, Lee R. Lynd and Daniel G. Olson
Anaerobic fermentation of lignocellulose occurs in both natural and managed environments, and is an essential part of the carbon cycle as well as a promising route to sustainable production of fuels and chemic...
Authors: Xiaoyu Liang, Jason M. Whitham, Evert K. Holwerda, Xiongjun Shao, Liang Tian, Yu-Wei Wu, Vincent Lombard, Bernard Henrissat, Dawn M. Klingeman, Zamin K. Yang, Mircea Podar, Tom L. Richard, James G. Elkins, Steven D. Brown and Lee R. Lynd
The microbial production of fatty acids has received great attention in the last few years as feedstock for the production of renewable energy. The main advantage of using cyanobacteria over other organisms is...
Authors: María Santos-Merino, M. Pilar Garcillán-Barcia and Fernando de la Cruz
Lignin is a potential feedstock for microbial conversion into various chemicals. However, the degradation rate of native or technical lignin is low, and depolymerization is needed to obtain reasonable conversi...
Authors: Krithika Ravi, Omar Y. Abdelaziz, Matthias Nöbel, Javier García-Hidalgo, Marie F. Gorwa-Grauslund, Christian P. Hulteberg and Gunnar Lidén
Production of biofuels and bioenergy precursors by phototrophic microorganisms, such as microalgae and cyanobacteria, is a promising alternative to conventional fuels obtained from non-renewable resources. Sev...
Authors: Juan D. Tibocha-Bonilla, Cristal Zuñiga, Rubén D. Godoy-Silva and Karsten Zengler
Increasing the efficiency of enzymatic biomass degradation is crucial for a more economically feasible conversion of abundantly available plant feedstock. Synergistic effects between the enzymes deployed in th...
Authors: Jonathan Herlet, Wolfgang H. Schwarz, Vladimir V. Zverlov, Wolfgang Liebl and Petra Kornberger
Replacing fossil fuels as energy carrier requires alternatives that combine sustainable production, high volumetric energy density, easy and fast refueling for mobile applications, and preferably low risk of h...
Authors: Fabian M. Schwarz, Kai Schuchmann and Volker Müller
Alkali used for pH control during fermentation and acidification for downstream recovery of succinic acid (SA) are the two largest cost contributors for bio-based SA production. To promote the commercializatio...
Authors: Chong Li, Shi Gao, Xiaotong Li, Xiaofeng Yang and Carol Sze Ki Lin
To reduce emissions from petrochemical refinement, bio-production has been heralded as a way to create economically valuable compounds with fewer harmful effects. For example, gaseous alkenes are precursor mol...
Authors: James Wilson, Sarah Gering, Jessica Pinard, Ryan Lucas and Brandon R. Briggs
Taking into account its thermotolerance, high growth rate, and broad substrate spectrum, Kluyveromyces marxianus can be considered an ideal consolidated bioprocessing (CBP). A major obstacle to ethanol production...
Authors: Jungang Zhou, Peixia Zhu, Xiaoyue Hu, Hong Lu and Yao Yu
Recently, it has been indicated that free nitrous acid (FNA) and Fenton pre-treatment of waste activated sludge can enhance methane production in anaerobic digestion of waste activated sludge. In addition, it ...
Authors: Seyed Mostafa Hallaji, Ali Torabian, Behnoush Aminzadeh, Soraya Zahedi and Nicky Eshtiaghi
The filamentous fungus Trichoderma reesei, the most widely used cellulase producer, also has promising applications in lignocellulose-based biorefinery: consolidated bioprocessing for the production of high value...
Authors: Guokun Wang, Wendi Jia, Na Chen, Ke Zhang, Lixian Wang, Pin Lv, Ronglin He, Min Wang and Dongyuan Zhang
Astaxanthin is a natural carotenoid pigment with tremendous antioxidant activity and great commercial value. Microbial production of astaxanthin via metabolic engineering has become a promising alternative. Al...
Authors: Jin Jin, Ying Wang, Mingdong Yao, Xiaoli Gu, Bo Li, Hong Liu, Mingzhu Ding, Wenhai Xiao and Yingjin Yuan
Bioethanol production processes with Saccharomyces cerevisiae using lignocellulosic biomass as feedstock are challenged by the simultaneous utilization of pentose and hexose sugars from biomass hydrolysates. The ...
Authors: Verena Oehling, Paul Klaassen, Oliver Frick, Christian Dusny and Andreas Schmid
The bioconversion of lignocellulosic biomass in various industrial processes, such as the production of biofuels, requires the degradation of hemicellulose. Clostridium stercorarium is a thermophilic bacterium, w...
Authors: Jannis Broeker, Matthias Mechelke, Melanie Baudrexl, Denise Mennerich, Daniel Hornburg, Matthias Mann, Wolfgang H. Schwarz, Wolfgang Liebl and Vladimir V. Zverlov
Microbial biosynthesis of alkanes is considered a promising method for the sustainable production of drop-in fuels and chemicals. Carbon dioxide would be an ideal carbon source for these production systems, bu...
Authors: Tapio Lehtinen, Henri Virtanen, Suvi Santala and Ville Santala
β-Glucosidases are components of the cellulase system, a family of enzymes that hydrolyze the β-1,4 linkages of cellulose. These proteins have been extensively studied due to the possibility of their use in vario...
Authors: Samara G. da Costa, Olinto Liparini Pereira, André Teixeira-Ferreira, Richard Hemmi Valente, Sebastião T. de Rezende, Valéria M. Guimarães and Fernando Ariel Genta
Microalgae are capable of producing sustainable bioproducts and biofuels by using carbon dioxide or other carbon substances in various cultivation modes. It is of great significance to exploit microalgae for t...
Authors: Han Sun, Weiyang Zhao, Xuemei Mao, Yuelian Li, Tao Wu and Feng Chen
Expression of glycosyl hydrolases in lignocellulosic biomass has been proposed as an alternative to improve efficiency of cellulosic ethanol production. In planta production of hyperthermophilic hydrolytic enzyme...
Authors: Yao Xiao, Xuejun He, Yemaiza Ojeda-Lassalle, Charleson Poovaiah and Heather D. Coleman
Propionic acid (PA) is used as a food preservative and increasingly, as a precursor for the synthesis of monomers. PA is produced mainly through hydrocarboxylation of ethylene, also known as the ‘oxo-process’;...
Authors: Carlos H. Luna-Flores, Chris C. Stowers, Brad M. Cox, Lars K. Nielsen and Esteban Marcellin
Developing novel microbial cell factories requires careful testing of candidates under industrially relevant conditions. However, this frequently occurs late during the strain development process. The availabi...
Authors: Felipe Senne de Oliveira Lino, Thiago Olitta Basso and Morten Otto Alexander Sommer
Whole-tree chips will be a likely feedstock for future biorefineries because of their low cost. Non-structural components (NSC), however, represent a significant part of whole-tree chips. The NSC can account f...
Authors: Hanna Hörhammer, Chang Dou, Rick Gustafson, Azra Suko and Renata Bura
Arabinoxylan is an abundant polysaccharide in industrially relevant biomasses such as sugarcane, corn stover and grasses. However, the arabinofuranosyl di-substitutions that decorate the xylan backbone are rec...
Authors: Camila Ramos dos Santos, Priscila Oliveira de Giuseppe, Flávio Henrique Moreira de Souza, Letícia Maria Zanphorlin, Mariane Noronha Domingues, Renan Augusto Siqueira Pirolla, Rodrigo Vargas Honorato, Celisa Caldana Costa Tonoli, Mariana Abrahão Bueno de Morais, Vanesa Peixoto de Matos Martins, Lucas Miranda Fonseca, Fernanda Büchli, Paulo Sergio Lopes de Oliveira, Fábio Cesar Gozzo and Mário Tyago Murakami
The development of efficient cellulase blends is a key factor for cost-effectively valorizing biomass in a new bio-economy. Today, the enzymatic hydrolysis of plant-derived polysaccharides is mainly accomplish...
Authors: Benedikt Leis, Claudia Held, Björn Andreeßen, Wolfgang Liebl, Sigrid Graubner, Louis-Philipp Schulte, Wolfgang H. Schwarz and Vladimir V. Zverlov
Cyanobacteria have shown promising potential for the production of various biofuels and chemical feedstocks. Synechococcus elongatus UTEX 2973 is a fast-growing strain with pronounced tolerance to high temperatur...
Authors: Xiaoming Tan, Shengwei Hou, Kuo Song, Jens Georg, Stephan Klähn, Xuefeng Lu and Wolfgang R. Hess
The thermophilic anaerobic bacterium Clostridium thermocellum is a multifunctional ethanol producer, capable of both saccharification and fermentation, that is central to the consolidated bioprocessing (CBP) appr...
Authors: Ninad Kothari, Evert K. Holwerda, Charles M. Cai, Rajeev Kumar and Charles E. Wyman
The diverse microbiome present in the rumen of ruminant animals facilitates the digestion of plant-based fiber. In this study, a shotgun metagenomic analysis of the microbes adhering to plant fiber in the came...
Authors: Javad Gharechahi and Ghasem Hosseini Salekdeh
Lavender (Lavandula angustifolia) and lavandin (a sterile hybrid of L. angustifolia × L. latifolia) essential oils are among those most commonly used in the world for various industrial purposes, including perfum...
Authors: Laurence Lesage-Meessen, Marine Bou, Christian Ginies, Didier Chevret, David Navarro, Elodie Drula, Estelle Bonnin, José C. del Río, Elise Odinot, Alexandra Bisotto, Jean-Guy Berrin, Jean-Claude Sigoillot, Craig B. Faulds and Anne Lomascolo
Lytic polysaccharide monooxygenase (LPMOs) are enzymes that catalyze the breakdown of polysaccharides in biomass and have excellent potential for biorefinery applications. However, their activities are relativ...
Authors: Damao Wang, Jing Li, Ann C. Y. Wong, Finn L. Aachmann and Yves S. Y. Hsieh
The lignin can compete for binding cellulase enzymes with cellulose fibers and decrease the accessibility of enzymes to carbohydrates. The competitive adsorption of cellulase to lignin mainly depended on the c...
Authors: Wenjun Ying, Zhengjun Shi, Haiyan Yang, Gaofeng Xu, Zhifeng Zheng and Jing Yang
Lignocellulose is highly recalcitrant to enzymatic deconstruction, where the recalcitrance primarily results from chemical linkages between lignin and carbohydrates. Glucuronoyl esterases (GEs) from carbohydra...
Authors: Jenny Arnling Bååth, Scott Mazurkewich, Rasmus Meland Knudsen, Jens-Christian Navarro Poulsen, Lisbeth Olsson, Leila Lo Leggio and Johan Larsbrink
During the process of bioethanol production, cellulose is hydrolyzed into its monomeric soluble units. For efficient hydrolysis, a chemical and/or mechanical pretreatment step is required. Such pretreatment is...
Authors: Shahar Yoav, Tomer M. Salame, Daria Feldman, Dana Levinson, Michael Ioelovich, Ely Morag, Oded Yarden, Edward A. Bayer and Yitzhak Hadar
