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  1. The limited tolerance of Saccharomyces cerevisiae to inhibitors is a major challenge in second-generation bioethanol production, and our understanding of the molecular mechanisms providing tolerance to inhibitor-...

    Authors: Marlous van Dijk, Peter Rugbjerg, Yvonne Nygård and Lisbeth Olsson

    Citation: Biotechnology for Biofuels 2021 14:201

    Content type: Research

    Published on:

  2. Isobutanol is considered a potential biofuel, thanks to its high-energy content and octane value, limited water solubility, and compatibility with gasoline. As its biosynthesis pathway is known, a microorganis...

    Authors: Hsien-Lin Liu, Christine H.-T. Wang, En-Pei Isabel Chiang, Chieh-Chen Huang and Wen-Hsiung Li

    Citation: Biotechnology for Biofuels 2021 14:200

    Content type: Research

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  3. Cellulase synthesized by fungi can environment-friendly and sustainably degrades cellulose to fermentable sugars for producing cellulosic biofuels, biobased medicine and fine chemicals. Great efforts have been...

    Authors: Ai-Ping Pang, Funing Zhang, Xin Hu, Yongsheng Luo, Haiyan Wang, Samran Durrani, Fu-Gen Wu, Bing-Zhi Li, Zhihua Zhou, Zuhong Lu and Fengming Lin

    Citation: Biotechnology for Biofuels 2021 14:199

    Content type: Research

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  4. Lacto-N-triose II (LNT II), an important backbone for the synthesis of different human milk oligosaccharides, such as lacto-N-neotetraose and lacto-N-tetraose, has recently received significant attention. The pro...

    Authors: Duoduo Hu, Hao Wu, Yingying Zhu, Wenli Zhang and Wanmeng Mu

    Citation: Biotechnology for Biofuels 2021 14:198

    Content type: Research

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  5. A platform for the utilization of the Chlorella sp. biomass and sugarcane leaves to produce multiple products (biorefinery concept) including hydrogen, methane, polyhydroxyalkanoates (PHAs), lipid, and soil suppl...

    Authors: Napapat Sitthikitpanya, Sureewan Sittijunda, Sontaya Khamtib and Alissara Reungsang

    Citation: Biotechnology for Biofuels 2021 14:197

    Content type: Research

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  6. Tissue heterogeneity significantly influences the overall saccharification efficiency of plant biomass. However, the mechanisms of specific organ or tissue recalcitrance to enzymatic deconstruction are general...

    Authors: Yufen Wang, Xianyang Xu, Huiting Xue, Dejian Zhang and Guanhua Li

    Citation: Biotechnology for Biofuels 2021 14:196

    Content type: Research

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  7. Xylanase, an important accessory enzyme that acts in synergy with cellulase, is widely used to degrade lignocellulosic biomass. Thermostable enzymes with good catalytic activity at lower temperatures have grea...

    Authors: Shuai You, Ziqian Zha, Jing Li, Wenxin Zhang, Zhiyuan Bai, Yanghao Hu, Xue Wang, Yiwen Chen, Zhongli Chen, Jun Wang and Huiying Luo

    Citation: Biotechnology for Biofuels 2021 14:195

    Content type: Research

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  8. Sunflower stalk pith, residue from the processing of sunflower, is rich in pectin and cellulose, thereby acting as an economic raw material for the acquisition of these compounds. In order to increase the comm...

    Authors: Qibo Zhang, Lu Cheng, Xutong Ma, Xin Zhou and Yong Xu

    Citation: Biotechnology for Biofuels 2021 14:194

    Content type: Research

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  9. Even if the loss of production capacity of a microorganism is said to be a serious problem in various biotechnology industries, reports in literature are rather rare. Strains of the genera Trichoderma reesei are ...

    Authors: R. Martzy, T. M. Mello-de-Sousa, R. L. Mach, D. Yaver and A. R. Mach-Aigner

    Citation: Biotechnology for Biofuels 2021 14:193

    Content type: Research

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  10. Ethyl acetate (C4H8O2) and hydrogen (H2) are industrially relevant compounds that preferably are produced via sustainable, non-petrochemical production processes. Both compounds are volatile and can be produced b...

    Authors: Anna C. Bohnenkamp, René H. Wijffels, Servé W. M. Kengen and Ruud A. Weusthuis

    Citation: Biotechnology for Biofuels 2021 14:192

    Content type: Research

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  11. Woody biomass has been considered as a promising feedstock for biofuel production via thermochemical conversion technologies such as fast pyrolysis. Extensive Life Cycle Assessment studies have been completed ...

    Authors: Kai Lan, Longwen Ou, Sunkyu Park, Stephen S. Kelley, Prakash Nepal, Hoyoung Kwon, Hao Cai and Yuan Yao

    Citation: Biotechnology for Biofuels 2021 14:191

    Content type: Research

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  12. In the oilseed crop Brassica napus (rapeseed), various metabolic processes influence seed oil content, oil quality, and biological yield. However, the role of plastid membrane proteins in these traits has not bee...

    Authors: Zhongchun Xiao, Fang Tang, Liyuan Zhang, Shengting Li, Shufeng Wang, Qiang Huo, Bo Yang, Chao Zhang, Daojie Wang, Qing Li, Lijuan Wei, Tao Guo, Cunmin Qu, Kun Lu, Yanfeng Zhang, Liang Guo…

    Citation: Biotechnology for Biofuels 2021 14:190

    Content type: Research

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  13. Lignin is a complex aromatic heteropolymer comprising 15–30% dry weight of the lignocellulose. The complex structural characteristic of lignin renders it difficult for value-added utilization. Exploring effici...

    Authors: Jiangshan Ma, Qiang Li, Yujie Wu, Huimin Yue, Yanghong Zhang, Jiashun Zhang, Muling Shi, Sixian Wang and Gao-Qiang Liu

    Citation: Biotechnology for Biofuels 2021 14:189

    Content type: Research

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  14. Sugar alcohols are widely used as low-calorie sweeteners in the food and pharmaceutical industries. They can also be transformed into platform chemicals. Yarrowia lipolytica, an oleaginous yeast, is a promising h...

    Authors: Sujit Sadashiv Jagtap, Ashwini Ashok Bedekar, Vijay Singh, Yong-Su Jin and Christopher V. Rao

    Citation: Biotechnology for Biofuels 2021 14:188

    Content type: Research

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  15. The industrial production of various alcohols from organic carbon compounds may be performed at high rates and with a low risk of contamination using thermophilic microorganisms as whole-cell catalysts. Thermoana...

    Authors: Lisa Hitschler, Laura Sofie Nissen, Michelle Kuntz and Mirko Basen

    Citation: Biotechnology for Biofuels 2021 14:187

    Content type: Research

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  16. Consolidated bioprocessing (CBP) technique is a promising strategy for biorefinery construction, producing bulk chemicals directly from plant biomass without extra hydrolysis steps. Fixing and channeling CO2 into...

    Authors: Jingen Li, Bingchen Chen, Shuying Gu, Zhen Zhao, Qian Liu, Tao Sun, Yongli Zhang, Taju Wu, Defei Liu, Wenliang Sun and Chaoguang Tian

    Citation: Biotechnology for Biofuels 2021 14:186

    Content type: Research

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  17. Recent research articles indicate that direct interspecies electron transfer (DIET) is an alternative metabolic route for methanogenic archaea that improves microbial methane productivity. It has been shown th...

    Authors: Kerstin Heitkamp, Adriel Latorre-Pérez, Sven Nefigmann, Helena Gimeno-Valero, Cristina Vilanova, Efri Jahmad and Christian Abendroth

    Citation: Biotechnology for Biofuels 2021 14:185

    Content type: Research

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  18. The green microalga Chromochloris zofingiensis is capable of producing high levels of triacylglycerol rich in C18 unsaturated fatty acids (UFAs). FA desaturation degree is regulated by FA desaturases (FADs). Neve...

    Authors: Tao Wu, Lihua Yu, Yu Zhang and Jin Liu

    Citation: Biotechnology for Biofuels 2021 14:184

    Content type: Research

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  19. Endo-β-1,4-galactanases are glycoside hydrolases (GH) from the GH53 family belonging to the largest clan of GHs, clan GH-A. GHs are ubiquitous and involved in a myriad of biological functions as well as being ...

    Authors: Sebastian J. Muderspach, Folmer Fredslund, Verena Volf, Jens-Christian Navarro Poulsen, Thomas H. Blicher, Mads Hartvig Clausen, Kim Krighaar Rasmussen, Kristian B. R. M. Krogh, Kenneth Jensen and Leila Lo Leggio

    Citation: Biotechnology for Biofuels 2021 14:183

    Content type: Research

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  20. Bio-hydrogen production via dark fermentation of low-value waste is a potent and simple mean of recovering energy, maximising the harvesting of reducing equivalents to produce the cleanest fuel amongst renewab...

    Authors: M. Arizzi, S. Morra, G. Gilardi, M. Pugliese, M. L. Gullino and F. Valetti

    Citation: Biotechnology for Biofuels 2021 14:182

    Content type: Research

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  21. Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates h...

    Authors: Zhi-Peng Wang, Xin-Yue Zhang, Yan Ma, Jing-Run Ye, Jing Jiang, Hai-Ying Wang and Wei Chen

    Citation: Biotechnology for Biofuels 2021 14:181

    Content type: Research

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  22. Microalgae are coming to the spotlight due to their potential applications in a wide number of fields ranging from the biofuel to the pharmaceutical sector. However, several factors such as low productivity, e...

    Authors: M. Giovannoni, I. Larini, V. Scafati, A. Scortica, M. Compri, D. Pontiggia, G. Zapparoli, N. Vitulo, M. Benedetti and B. Mattei

    Citation: Biotechnology for Biofuels 2021 14:180

    Content type: Research

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  23. Environmental factors, such as weather extremes, have the potential to cause adverse effects on plant biomass quality and quantity. Beyond adversely affecting feedstock yield and composition, which have been e...

    Authors: Meenaa Chandrasekar, Leela Joshi, Karleigh Krieg, Sarvada Chipkar, Emily Burke, Derek J. Debrauske, Kurt D. Thelen, Trey K. Sato and Rebecca G. Ong

    Citation: Biotechnology for Biofuels 2021 14:179

    Content type: Research

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  24. In terms of global demand, rapeseed is the third-largest oilseed crop after soybeans and palm, which produces vegetable oil for human consumption and biofuel for industrial production. Roots are vital organs f...

    Authors: Keqi Li, Jie Wang, Lieqiong Kuang, Ze Tian, Xinfa Wang, Xiaoling Dun, Jinxing Tu and Hanzhong Wang

    Citation: Biotechnology for Biofuels 2021 14:178

    Content type: Research

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  25. To create an ideotype woody bioenergy crop with desirable growth and biomass properties, we utilized the viral 2A-meidated bicistronic expression strategy to express both PtrMYB3 (MYB46 ortholog of Populus tricho...

    Authors: Jin-Seong Cho, Min-Ha Kim, Eun-Kyung Bae, Young-Im Choi, Hyung-Woo Jeon, Kyung-Hwan Han and Jae-Heung Ko

    Citation: Biotechnology for Biofuels 2021 14:177

    Content type: Research

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  26. The efficiency of biological systems as an option for pretreating lignocellulosic biomass has to be improved to make the process practical. Fungal treatment with manganese (Mn) addition for improving lignocell...

    Authors: Xiao Fu, Jialong Zhang, Xiangyu Gu, Hongbo Yu and Shulin Chen

    Citation: Biotechnology for Biofuels 2021 14:176

    Content type: Research

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  27. Lignification of secondary cell walls is a major factor conferring recalcitrance of lignocellulosic biomass to deconstruction for fuels and chemicals. Genetic modification can reduce lignin content and enhance...

    Authors: Hasi Yu, Chang Liu and Richard A. Dixon

    Citation: Biotechnology for Biofuels 2021 14:175

    Content type: Methodology

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  28. Isobutanol is an attractive biofuel with many advantages. Third-generation biorefineries that convert CO2 into bio-based fuels have drawn considerable attention due to their lower feedstock cost and more ecofrien...

    Authors: Xiao-Xi Wu, Jian-Wei Li, Su-Fang Xing, Hui-Ting Chen, Chao Song, Shu-Guang Wang and Zhen Yan

    Citation: Biotechnology for Biofuels 2021 14:174

    Content type: Research

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  29. The fermentation of lignocellulose hydrolysates to ethanol requires robust xylose-capable Saccharomyces cerevisiae strains able to operate in the presence of microbial inhibitory stresses. This study aimed at dev...

    Authors: Bianca A. Brandt, Maria D. P. García-Aparicio, Johann F. Görgens and Willem H. van Zyl

    Citation: Biotechnology for Biofuels 2021 14:173

    Content type: Research

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  30. Over the last few years, valorization of lignocellulosic biomass has been expanded beyond the production of second-generation biofuels to the synthesis of numerous platform chemicals to be used instead of thei...

    Authors: Grigorios Dedes, Anthi Karnaouri, Asimina A. Marianou, Konstantinos G. Kalogiannis, Chrysoula M. Michailof, Angelos A. Lappas and Evangelos Topakas

    Citation: Biotechnology for Biofuels 2021 14:172

    Content type: Research

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  31. Sugarcane bagasse (SCB) is an abundant feedstock for second-generation bioethanol production. This complex biomass requires an array of carbohydrate active enzymes (CAZymes), mostly from filamentous fungi, for...

    Authors: Olusola A. Ogunyewo, Pooja Upadhyay, Girish H. Rajacharya, Omoaruemike E. Okereke, Laura Faas, Leonardo D. Gómez, Simon J. McQueen-Mason and Syed Shams Yazdani

    Citation: Biotechnology for Biofuels 2021 14:171

    Content type: Research

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  32. Biogas can be upgraded to methane biologically by adding H2 to biogas reactors. The process is called biological methanation (BM) and can be done in situ in a regular biogas reactor or the biogas can be transferr...

    Authors: Radziah Wahid and Svein Jarle Horn

    Citation: Biotechnology for Biofuels 2021 14:170

    Content type: Research

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  33. Filamentous fungi are excellent lignocellulose degraders, which they achieve through producing carbohydrate active enzymes (CAZymes). CAZyme production is highly orchestrated and gene expression analysis has g...

    Authors: Raphael Gabriel, Rebecca Mueller, Lena Floerl, Cynthia Hopson, Simon Harth, Timo Schuerg, Andre Fleissner and Steven W. Singer

    Citation: Biotechnology for Biofuels 2021 14:169

    Content type: Research

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  34. Rhodospirillum rubrum is a purple non-sulphur bacterium that produces H2 by photofermentation of several organic compounds or by water gas-shift reaction during CO fermentation. Successful strategies for both pro...

    Authors: Alberto Rodríguez, Natalia Hernández-Herreros, José L. García and M. Auxiliadora Prieto

    Citation: Biotechnology for Biofuels 2021 14:168

    Content type: Research

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  35. Plants inherently display a rich diversity in cell wall chemistry, as they synthesize an array of polysaccharides along with lignin, a polyphenolic that can vary dramatically in subunit composition and interun...

    Authors: Lisanne de Vries, Sydne Guevara-Rozo, MiJung Cho, Li-Yang Liu, Scott Renneckar and Shawn D. Mansfield

    Citation: Biotechnology for Biofuels 2021 14:167

    Content type: Review

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  36. As the fifth major cereal crop originated from Africa, sorghum (Sorghum bicolor) has become a key C4 model organism for energy plant research. With the development of high-throughput detection technologies for va...

    Authors: Yuanming Liu, Zhonghuang Wang, Xiaoyuan Wu, Junwei Zhu, Hong Luo, Dongmei Tian, Cuiping Li, Jingchu Luo, Wenming Zhao, Huaiqing Hao and Hai-Chun Jing

    Citation: Biotechnology for Biofuels 2021 14:165

    Content type: Research

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  37. Biomass recalcitrance is governed by various molecular and structural factors but the interplay between these multiscale factors remains unclear. In this study, hot water pretreatment (HWP) was applied to maiz...

    Authors: Amandine Leroy, Xavier Falourd, Loïc Foucat, Valérie Méchin, Fabienne Guillon and Gabriel Paës

    Citation: Biotechnology for Biofuels 2021 14:164

    Content type: Research

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  38. As a potential source of polyunsaturated fatty acids (PUFA), Schizochytrium sp. has been widely used in industry for PUFA production. Polyketide synthase (PKS) cluster is supposed to be the primary way of PUFA sy...

    Authors: Yanyan Shi, Zhen Chen, Yixin Li, Xingyu Cao, Lijie Yang, Yiyuan Xu, Zhipeng Li and Ning He

    Citation: Biotechnology for Biofuels 2021 14:163

    Content type: Research

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  39. As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin d...

    Authors: Tangwu Cui, Bo Yuan, Haiwei Guo, Hua Tian, Weimin Wang, Yingqun Ma, Changzhi Li and Qiang Fei

    Citation: Biotechnology for Biofuels 2021 14:162

    Content type: Research

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  40. Fungal glucose dehydrogenases (GDHs) are FAD-dependent enzymes belonging to the glucose-methanol-choline oxidoreductase superfamily. These enzymes are classified in the “Auxiliary Activity” family 3 (AA3) of t...

    Authors: Gabriele Cerutti, Elena Gugole, Linda Celeste Montemiglio, Annick Turbé-Doan, Dehbia Chena, David Navarro, Anne Lomascolo, François Piumi, Cécile Exertier, Ida Freda, Beatrice Vallone, Eric Record, Carmelinda Savino and Giuliano Sciara

    Citation: Biotechnology for Biofuels 2021 14:161

    Content type: Research

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  41. Population increase and industrialization has resulted in high energy demand and consumptions, and presently, fossil fuels are the major source of staple energy, supplying 80% of the entire consumption. This h...

    Authors: Kehinde Oladoke Olatunji, Noor A. Ahmed and Oyetola Ogunkunle

    Citation: Biotechnology for Biofuels 2021 14:159

    Content type: Review

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  42. Future expansion of corn-derived ethanol raises concerns of sustainability and competition with the food industry. Therefore, cellulosic biofuels derived from agricultural waste and dedicated energy crops are ...

    Authors: Shannon M. Hoffman, Maria Alvarez, Gilad Alfassi, Dmitry M. Rein, Sergio Garcia-Echauri, Yachin Cohen and José L. Avalos

    Citation: Biotechnology for Biofuels 2021 14:157

    Content type: Research

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  43. Production of biodiesel from renewable sources such as inedible vegetable oils by enzymatic catalysis has been a hotspot but remains a challenge on the efficient use of an enzyme. COFs (Covalent Organic Framew...

    Authors: Zi-Wen Zhou, Chun-Xian Cai, Xiu Xing, Jun Li, Zu-E. Hu, Zong-Bo Xie, Na Wang and Xiao-Qi Yu

    Citation: Biotechnology for Biofuels 2021 14:156

    Content type: Research

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  44. Saccharomyces cerevisiae is widely used in traditional brewing and modern fermentation industries to produce biofuels, chemicals and other bioproducts, but challenged by various harsh industrial conditions, such ...

    Authors: Yanfang Liu, Yuping Lin, Yufeng Guo, Fengli Wu, Yuanyuan Zhang, Xianni Qi, Zhen Wang and Qinhong Wang

    Citation: Biotechnology for Biofuels 2021 14:155

    Content type: Research

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  45. The recalcitrance of lignocellulosic biomass is a major constraint to its high-value use at industrial scale. In nature, microbes play a crucial role in biomass degradation, nutrient recycling and ecosystem fu...

    Authors: Sivasamy Sethupathy, Gabriel Murillo Morales, Yixuan Li, Yongli Wang, Jianxiong Jiang, Jianzhong Sun and Daochen Zhu

    Citation: Biotechnology for Biofuels 2021 14:154

    Content type: Review

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  46. Sugarcane processing roughly generates 54 million tonnes sugarcane bagasse (SCB)/year, making SCB an important material for upgrading to value-added molecules. In this study, an integrated scheme was developed...

    Authors: Leila Khaleghipour, Javier A. Linares-Pastén, Hamid Rashedi, Seyed Omid Ranaei Siadat, Andrius Jasilionis, Said Al-Hamimi, Roya R. R. Sardari and Eva Nordberg Karlsson

    Citation: Biotechnology for Biofuels 2021 14:153

    Content type: Research

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  47. Sugarcane is an essential crop for sugar and ethanol production. Immediate processing of sugarcane is necessary after harvested because of rapid sucrose losses and deterioration of stalks. This study was condu...

    Authors: Na Peng, Ziting Yao, Ziting Wang, Jiangfeng Huang, Muhammad Tahir Khan, Baoshan Chen and Muqing Zhang

    Citation: Biotechnology for Biofuels 2021 14:152

    Content type: Research

    Published on: