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Table 2 Substrate consumption and product formation parameters during anaerobic batch fermentation of a mixture of glucose, arabinose and xylose in defined mineral medium

From: Comparing the xylose reductase/xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting Saccharomyces cerevisiae strains

  TMB3075 (xylose reductase/xylitol dehydrogenase) TMB3076 (xylose isomerase)
Consumed arabinose g l-1 4.04 ± 0.33 1.65 ± 0.01
Consumed xylose g l-1 20.1 ± 0.2 4.9 ± 0.7
q arabinose* (g arabinose) (g cells)-1 h-1 0.014 ± 0.001 0.005 ± 0.001
q xylose* (g xylose) (g cells)-1 h-1 0.09 ± 0.001 0.02 ± 0.001
Final ethanol titre g l-1 14.7 ± 0.5 11.8 ± 0.3
q ethanol* (g ethanol) (g cells)-1 h-1 0.024 ± 0.001 0.010 ± 0.001
Y ethanol, on total added sugars (g ethanol) (g sugar)-1 0.23 ± 0.01 0.18 ± 0.01
Y ethanol, on consumed sugars (g ethanol) (g sugar)-1 0.32 ± 0.01 0.41 ± 0.01
Y xylitol (g xylitol) (g consumed xylose)-1 0.27 ± 0.04 0.04 ± 0.02
Y arabitol (g arabitol) (g consumed arabinose)-1 0.87 ± 0.03 ND
Y biomass, on total added sugars
(g dry cell weight) (g sugar) -1
0.03 ± 0.01 0.03 ± 0.01
Y biomass, on consumed sugars
(g dry cell weight) (g sugar) -1
0.04 ± 0.01 0.07 ± 0.01
Y glycerol (g acetate) (g consumed arabinose)-1 0.08 ± 0.001 0.09 ± 0.01
Y acetate (g acetate) (g consumed arabinose)-1 0.020 ± 0.002 0.016 ± 0.001
  1. Values are the calculated average of two biological replicates. *Calculated after glucose depletion.
  2. ND.: not determined. q: specific productivity, Y: yield.