Growth curves with different glucose concentrations. Growth curves of E. coli crp*-gfp grown in modified 1 × M9 minimal media containing 2, 4, or 8% glucose. All points are the mean of four replications with error bars indicating the standard error. (a) Data was fit to curves described by the Gompertz equation. R2 values: 2%, 0.85; 4%, 0.91; 8%, 0.97. (b) Each replication consisted of the fluorescence of a crp*-gfp- culture subtracted from the fluorescence of a crp*-gfp culture. Data was fit to first derivative Gompertz equation. R2 values: 2%, 0.80; 4%, 0.82; 8%, 0.82. (c) Mean fluorescence values from panel b were integrated and plotted against mean crp*-gfp absorbance values from panel a. Linear fit R2 values: 2%, 0.90; 4%, 0.93; 8%, 0.98 (n = 4).