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Table 6 Fermentation parameters of 1 l batch cultures of C. saccharolyticus at 72°C and T. neapolitana at 80°C grown on a glucose/xylose (7:3, w w-1) mixture and on Miscanthus hydrolysate.

From: Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana

Mono-saccharides Ferm. period Consumption Production C-balance COD-balance
    mmol l-1   mmol l-1 g l-1   
g l-1 h Glucose Xylose COD H2 CO2 Acetate Lactate COD CDW   
C. saccharolyticus             
Glc/Xyl             
10 28 39.3 18.7   188 100 88.8 1.1   0.67 0.94 0.93
14 34 55.2 25.4   253 130 117 1.9 7.4 0.77 0.87 0.91
28 >71 77.4 54.8   293 171 136 3.7 53.1 0.84 0.66 0.79
Hydrolysate             
10 35 41.9 17.5 82.6 237 120 105 1.1   0.98 0.89 0.92
14 69 58.9 26.2 92.8 319 176 152 2.3   1.04 0.92 0.92
28 >71 52.4 41.5 97.9 244 153 125 4.5   1.01 0.74 0.88
T. neapolitana             
Glc/Xyl             
10 22 38.2 18.6   178 93.6 82.3 3.9   0.97 0.96 0.94
14 33 54.4 24.4   240 131 105 5.9 15.8 0.74 0.87 0.91
28 >45 66.7 21.3   189 113 89.9 14.2 58.4 0.42 0.81 0.90
Hydrolysate             
10 26 40.8 16.0 80.3 198 102 88.0 9.6   1.27 0.89 0.92
14 39 55.8 22.2 94.3 285 154 124 11.2   1.02 0.88 0.91
28 >45 42.9 22.8 -a 82.0 62.2 45.2 11.2 124 0.46 0.89 0.96
  1. aDue to Maillard reactions and thus non-defined product formation, no net chemical oxygen demand (COD) consumption was measured.
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