|
Substrate
|
Enzyme
|
Final glucose (g/L)
|
Final xylose (g/L)
|
Glucose from hydrolysis (g/L)
|
Xylose from hydrolysis (g/L)
|
Glucose yield (% of theoreticala)
|
Xylose yield (% of theoreticala)
|
|---|
|
SPS
|
Cell+Nov
|
6.9
|
1.4
|
3.6
|
n.d.
|
29
|
-
|
| |
F-1663/S
|
6.9
|
1.4
|
3.6
|
n.d.
|
29
|
-
|
| |
F-1663/WS
|
6.8
|
1.4
|
3.5
|
n.d.
|
28
|
-
|
| |
F-1663/B
|
6.9
|
1.4
|
3.6
|
n.d.
|
29
|
-
|
|
SPWS
|
Cell+Nov
|
9.6
|
6.5
|
8.7
|
1.1
|
60
|
72
|
| |
F-1663/S
|
10.4
|
5.6
|
9.5
|
0.2
|
65
|
13
|
| |
F-1663/WS
|
10.3
|
5.6
|
9.4
|
0.2
|
64
|
13
|
| |
F-1663/B
|
10.0
|
5.6
|
9.1
|
0.2
|
62
|
13
|
|
SPB
|
Cell+Nov
|
7.6
|
5.6
|
6.8
|
3.0
|
50
|
53
|
| |
F-1663/S
|
7.6
|
3.0
|
6.8
|
0.4
|
50
|
7
|
| |
F-1663/WS
|
8.3
|
3.1
|
7.5
|
0.5
|
55
|
9
|
| |
F-1663/B
|
8.0
|
3.3
|
7.2
|
0.7
|
52
|
12
|
- SPS: steam-pretreated spruce; SPWS: steam-pretreated wheat straw; SPB: steam-pretreated sugarcane bagasse
- a Theoretical sugar concentrations from hydrolysis (glucose and xylose originating from glucan + glucose oligomers and xylan + xylose oligomers, respectively) were 12.3 g/L glucose on SPS, 14.6 g/L glucose and 1.5 g/L xylose on SPWS, and 13.7 g/L glucose and 5.7 g/L xylose on SPB.
- n.d.: not detected, Cell+Nov: the 3:1 (w:w) mixture of Celluclast 1.5 L & Novozym 188, F-1663/S, WS, B: the crude enzyme supernatants of Trichoderma atroviride TUB F-1663 produced on steam-pretreated spruce, wheat straw and sugarcane bagasse.
- The values presented are the average of two separate hydrolysis experiments, and the standard deviations are <5%.
