Enzymatic hydrolysis of steam-pretreated lignocellulosic materials with Trichoderma atroviride enzymes produced in-house

Table 4 Final concentrations and yields of glucose and xylose obtained in the hydrolysis of SPS, SPWS and SPB

Substrate	Enzyme	Final glucose (g/L)	Final xylose (g/L)	Glucose from hydrolysis (g/L)	Xylose from hydrolysis (g/L)	Glucose yield (% of theoretical^a)	Xylose yield (% of theoretical^a)
SPS	Cell+Nov	6.9	1.4	3.6	n.d.	29	-
	F-1663/S	6.9	1.4	3.6	n.d.	29	-
	F-1663/WS	6.8	1.4	3.5	n.d.	28	-
	F-1663/B	6.9	1.4	3.6	n.d.	29	-
SPWS	Cell+Nov	9.6	6.5	8.7	1.1	60	72
	F-1663/S	10.4	5.6	9.5	0.2	65	13
	F-1663/WS	10.3	5.6	9.4	0.2	64	13
	F-1663/B	10.0	5.6	9.1	0.2	62	13
SPB	Cell+Nov	7.6	5.6	6.8	3.0	50	53
	F-1663/S	7.6	3.0	6.8	0.4	50	7
	F-1663/WS	8.3	3.1	7.5	0.5	55	9
	F-1663/B	8.0	3.3	7.2	0.7	52	12

SPS: steam-pretreated spruce; SPWS: steam-pretreated wheat straw; SPB: steam-pretreated sugarcane bagasse
^a Theoretical sugar concentrations from hydrolysis (glucose and xylose originating from glucan + glucose oligomers and xylan + xylose oligomers, respectively) were 12.3 g/L glucose on SPS, 14.6 g/L glucose and 1.5 g/L xylose on SPWS, and 13.7 g/L glucose and 5.7 g/L xylose on SPB.
n.d.: not detected, Cell+Nov: the 3:1 (w:w) mixture of Celluclast 1.5 L & Novozym 188, F-1663/S, WS, B: the crude enzyme supernatants of Trichoderma atroviride TUB F-1663 produced on steam-pretreated spruce, wheat straw and sugarcane bagasse.
The values presented are the average of two separate hydrolysis experiments, and the standard deviations are <5%.

ISSN: 2731-3654