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Table 4 Final concentrations and yields of glucose and xylose obtained in the hydrolysis of SPS, SPWS and SPB

From: Enzymatic hydrolysis of steam-pretreated lignocellulosic materials with Trichoderma atroviride enzymes produced in-house

Substrate Enzyme Final glucose (g/L) Final xylose (g/L) Glucose from hydrolysis (g/L) Xylose from hydrolysis (g/L) Glucose yield (% of theoreticala) Xylose yield (% of theoreticala)
SPS Cell+Nov 6.9 1.4 3.6 n.d. 29 -
  F-1663/S 6.9 1.4 3.6 n.d. 29 -
  F-1663/WS 6.8 1.4 3.5 n.d. 28 -
  F-1663/B 6.9 1.4 3.6 n.d. 29 -
SPWS Cell+Nov 9.6 6.5 8.7 1.1 60 72
  F-1663/S 10.4 5.6 9.5 0.2 65 13
  F-1663/WS 10.3 5.6 9.4 0.2 64 13
  F-1663/B 10.0 5.6 9.1 0.2 62 13
SPB Cell+Nov 7.6 5.6 6.8 3.0 50 53
  F-1663/S 7.6 3.0 6.8 0.4 50 7
  F-1663/WS 8.3 3.1 7.5 0.5 55 9
  F-1663/B 8.0 3.3 7.2 0.7 52 12
  1. SPS: steam-pretreated spruce; SPWS: steam-pretreated wheat straw; SPB: steam-pretreated sugarcane bagasse
  2. a Theoretical sugar concentrations from hydrolysis (glucose and xylose originating from glucan + glucose oligomers and xylan + xylose oligomers, respectively) were 12.3 g/L glucose on SPS, 14.6 g/L glucose and 1.5 g/L xylose on SPWS, and 13.7 g/L glucose and 5.7 g/L xylose on SPB.
  3. n.d.: not detected, Cell+Nov: the 3:1 (w:w) mixture of Celluclast 1.5 L & Novozym 188, F-1663/S, WS, B: the crude enzyme supernatants of Trichoderma atroviride TUB F-1663 produced on steam-pretreated spruce, wheat straw and sugarcane bagasse.
  4. The values presented are the average of two separate hydrolysis experiments, and the standard deviations are <5%.
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