Figure 5

Fermentation using (A) Zymomonas mobilis mobilis AX101, (B) Saccharomyces cerevisiae 424A(LNH-ST) and (C) Escherichia coli KO11, in enzymatic hydrolysate from 6.0% glucan loading of ammonia fiber explosion (AFEX)-pretreated corn stover (CS). Fermentation was conducted under largely anaerobic condition and initiated at cell density equivalent to 0.5 unit OD600 nm temperature and pH were controlled at 37°C, 6.8 for KO11 and 30°C, 5.5 for AX101 and 424A(LNH-ST). Solid lines: Seed culture in yeast extract peptone; dotted lines: seed culture in 3% glucan loading of AFEX-CS hydrolysate.