Table 2 Results for fermentation using Escherichia coli KO11, Zymomonas mobilis mobilis AX101 and Saccharomyces cerevisiae 424A(LNH-ST) in 2% w/w corn steep liquor (CSL) or yeast extract peptone (YEP)
Nutrients Source/sugar Type | Concentration (g/L) | Strain | Sugar consumption (%)* | Metabolic EtOH yield (%)*†| Carbon balance coverage‡ | EtOH Concentration* | Volumetric productivity (g/L/hr)†| Specific productivity (g/L/h/g cell)†0--48 h | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | Â | Â | Â | Â | Glc 0-24 h | Xyl 0-48 h | EtOH 0-48 h | Â |
|  |  | KO11 | 100.0 ± 0.0 | 87.0 ± 0.9 | 97.3 ± 1.1 | 44.3 ± 0.5 | -2.37 ± 0.00 | N/A | 0.79 | 0.57 ± 0.03 |
CSL/glucose | 100 | AX101 | 100.0 ± 0.0 | 93.2 ± 0.1 | 95.1 ± 0.1 | 47.5 ± 0.1 | -3.64 ± 0.13 | N/A | 0.97 | 0.77 ± 0.07 |
|  |  | 424A(LHN-ST) | 100.0 ± 0.0 | 85.2 ± 0.5 | 95.8 ± 0.4 | 43.5 ± 0.3 | -4.16 ± 0.03 | N/A | 0.87 | 0.16 ± 0.00 |
|  |  | KO11 | 98.2 ± 0.5 | 85.1 ± 1.1 | 95.9 ± 1.1 | 41.9 ± 0.8 | -2.16 ± 0.07 | -0.38 ± 0.03 | +0.72 ± 0.01 | 0.55 ± 0.00 |
CSL/glucose + xylose | 70 + 30 | AX101 | 94.5 ± 2.4 | 88.6 ± 0.0 | 96.0 ± 0.0 | 41.5 ± 1.1 | -2.43 ± 0.37 | -0.29 ± 0.05 | +0.77 ± 0.02 | 0.69 ± 0.02 |
|  |  | 424A(LHN-ST) | 98.4 ± 0.5 | 82.4 ± 0.5 | 100.3 ± 0.5 | 40.2 ± 0.1 | -2.76 ± 0.08 | -0.39 ± 0.04 | +0.73 ± 0.01 | 0.13 ± 0.00 |
|  |  | KO11 | 100.0 ± 0.0 | 85.1 ± 0.0 | 93.6 ± 0.2 | 43.1 ± 0.1 | N/A | -1.74 ± 0.02 | +0.72 ± 0.01 | 0.54 ± 0.00 |
CSL/Xylose | 100 | AX101 | 95.3 ± 0.1 | 84.9 ± 0.2 | 97.1 ± 0.1 | 41.3 ± 0.1 | N/A | -1.04 ± 0.01 | +0.42 ± 0.00 | 0.46 ± 0.01 |
|  |  | 424A(LHN-ST) | 37.9 ± 6.5 | 89.8 ± 1.3 | 93.2 ± 0.9 | 18.1 ± 3.2 | N/A | -0.32 ± 0.05 | +0.09 ± 0.02 | 0.10 ± 0.00 |
YEP§/xylose | 100 | 424A(LHN-ST) | 100.0 ± 0.0 | 92.0 ± 0.01 | N/A | 46.9 ± 0.0 | N/A | -1.77 ± 0.02 | +0.87 ± 0.01 | 0.11 ± 0.00 |