Release of sugars from tobacco lignin mutants at different incubation times. Samples from wild type, a double mutant for cinnamyl alcohol dehydrogenase (CAD) and caffeate O-methyltransferase (COMT) (cadcomt), double cinnamoyl-CoA reductase (CCR) and COMT material (ccrcomt) and a CCR mutant line (ccr) were loaded in 96-well plates and analysed using the high throughput platform. Samples were taken after 2, 4, 8 and 16 hours of incubation with hydrolytic enzymes. (A) Glucose, (B) mannose, (C) xylose and (D) galactose A were quantified by high performance anion exchange chromatography. Bars represent values ± SD of experiments carried out in triplicate.