Figure 8
Localization of E1 in transgenic corn stover. Confocal microscopic images of sectioned WT and E1-containing corn stover. All images were obtained by using immunofluorescence confocal laser microscopy. An E1cd primary antibody and an Alexa Fluor 488 antimouse secondary antibody were used and spectrally deconvoluted to show antibody in red and nonspecific autofluoresence in green. (a) Original magnification ×200 image of E1-transgenic maize leaf tissue indicating that the transgenic tissue exhibits a broad expression of E1 throughout the plant. Scale bar, 50 μm. (b) Original magnification ×200 confocal microscopic image of leaf tissue from a WT control corn stover stalk showing no expression of E1 enzyme. Scale bar, 50 μm. (c) Original magnification ×600 image of E1-transgenic maize stem tissue. This image indicates that E1-transgenic tissue exhibits specific expression of E1 in the lignified sclerenchyma cells in the bottom portion of the image. Scale bar, 25 μm. (d) Original magnification ×600 confocal microscopic image of stem tissue from a WT control corn stover stalk showing no expression of E1 enzyme. Scale bar, 25 μm.