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Table 1 Purified cellulase activity in culture with rice strawa

From: Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses

  Culture supernatant Concentration + buffer exchange QFF anion exchange S200 gel filtration P1 S200 gel filtration P2
Fraction volume, mL 500 3 4 1 1
Total protein, mg 27.81 8.97 2.24 1.39 0.23
4-MUC      
   Total activity, U 470.71 196.33 67.21 26.17 17.94
   Specific activity, U/mg 16.93 21.90 29.95 18.89 78.97
   Yield, % 100% 42% 14% 6% 4%
   Purification factor 1.0 1.3 1.8 1.1 4.7
p NPG      
   Total activity, U 22.54 5.57 1.91 0.86 0.49
   Specific activity, U/mg 0.81 0.62 0.85 0.62 2.17
   Yield, % 100% 25% 8% 4% 2%
   Purification factor 1.0 0.8 1.1 0.8 2.7
dye-CMC      
   Total activity, U 16.79 3.36 0.78 0.07 0.26
   Specific activity, U/mg 0.60 0.38 0.35 0.05 1.15
   Yield, % 100% 20% 5% 0% 2%
   Purification factor 1.0 0.6 0.6 0.1 1.9
  1. a4-MUC: 4-methylumbelliferyl-β-cellobiose; dye-CMC: dye CM-cellulose; p NPG: p-nitrophenyl-β-D-glucopyranoside;
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