Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses

Table 1 Purified cellulase activity in culture with rice straw^a

	Culture supernatant	Concentration + buffer exchange	QFF anion exchange	S200 gel filtration P1	S200 gel filtration P2
Fraction volume, mL	500	3	4	1	1
Total protein, mg	27.81	8.97	2.24	1.39	0.23
4-MUC
Total activity, U	470.71	196.33	67.21	26.17	17.94
Specific activity, U/mg	16.93	21.90	29.95	18.89	78.97
Yield, %	100%	42%	14%	6%	4%
Purification factor	1.0	1.3	1.8	1.1	4.7
p NPG
Total activity, U	22.54	5.57	1.91	0.86	0.49
Specific activity, U/mg	0.81	0.62	0.85	0.62	2.17
Yield, %	100%	25%	8%	4%	2%
Purification factor	1.0	0.8	1.1	0.8	2.7
dye-CMC
Total activity, U	16.79	3.36	0.78	0.07	0.26
Specific activity, U/mg	0.60	0.38	0.35	0.05	1.15
Yield, %	100%	20%	5%	0%	2%
Purification factor	1.0	0.6	0.6	0.1	1.9

^a4-MUC: 4-methylumbelliferyl-β-cellobiose; dye-CMC: dye CM-cellulose; p NPG: p-nitrophenyl-β-D-glucopyranoside;

ISSN: 2731-3654