| |
Culture supernatant
|
Concentration + buffer exchange
|
QFF anion exchange
|
S200 gel filtration P1
|
S200 gel filtration P2
|
|---|
|
Fraction volume, mL
|
500
|
3
|
4
|
1
|
1
|
|
Total protein, mg
|
27.81
|
8.97
|
2.24
|
1.39
|
0.23
|
|
4-MUC
| | | | | |
|
Total activity, U
|
470.71
|
196.33
|
67.21
|
26.17
|
17.94
|
|
Specific activity, U/mg
|
16.93
|
21.90
|
29.95
|
18.89
|
78.97
|
|
Yield, %
|
100%
|
42%
|
14%
|
6%
|
4%
|
|
Purification factor
|
1.0
|
1.3
|
1.8
|
1.1
|
4.7
|
|
p NPG
| | | | | |
|
Total activity, U
|
22.54
|
5.57
|
1.91
|
0.86
|
0.49
|
|
Specific activity, U/mg
|
0.81
|
0.62
|
0.85
|
0.62
|
2.17
|
|
Yield, %
|
100%
|
25%
|
8%
|
4%
|
2%
|
|
Purification factor
|
1.0
|
0.8
|
1.1
|
0.8
|
2.7
|
|
dye-CMC
| | | | | |
|
Total activity, U
|
16.79
|
3.36
|
0.78
|
0.07
|
0.26
|
|
Specific activity, U/mg
|
0.60
|
0.38
|
0.35
|
0.05
|
1.15
|
|
Yield, %
|
100%
|
20%
|
5%
|
0%
|
2%
|
|
Purification factor
|
1.0
|
0.6
|
0.6
|
0.1
|
1.9
|
- a4-MUC: 4-methylumbelliferyl-β-cellobiose; dye-CMC: dye CM-cellulose; p NPG: p-nitrophenyl-β-D-glucopyranoside;
