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Table 6 Minimum cellulase and β-glucosidase loadings required for efficient hydrolysis (70% glucan conversion) of a broad range of pretreated lignocellulosic substrates as predicted by the equations shown in Table (5) for 2% (w/v) solids loading and 72 hours.

From: Cellulose accessibility limits the effectiveness of minimum cellulase loading on the efficient hydrolysis of pretreated lignocellulosic substrates

Substrate

Cell.a

BGb

Cell./BG

Total

SPCFc

5

18

0.3

23

SPCSd

30

24

1.3

54

SPLPe

42

21

2.0

63

SPDFf

45

16

2.8

61

OPPg

38

10

3.8

48

OPCFh

14

4

3.5

18

OPLPi

32

11

2.9

43

  1. aCell = cellulase (Celluclast 1.5), mg protein/g glucan.
  2. bBG = β-glucosidase (Novozym 188) mg protein/g glucan.
  3. cSPCF = steam-pretreated corn fiber.
  4. dSPCS = steam-pretreated corn stover.
  5. eSPLP = steam-pretreated lodgepole pine.
  6. fSPDF = steam-pretreated douglas fir.
  7. gOPP = organosolv-pretreated poplar.
  8. hOPCF = organosolv-pretreated corn fiber.
  9. iOPLP = organosolv-pretreated lodgepole pine.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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