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Table 1 Metabolite production in all cultures: cumulative gas production and accumulation of metabolites in the test cultures after 49 day of incubation

From: Biogenic hydrogen and methane production from Chlorella vulgaris and Dunaliella tertiolectabiomass

Substrate Inoculum H2(ml) CH4(ml) CO2(ml) Sum of VFA and alcohols (mM)
None U-C 0.0 ± 0.0 0.2 ± 0.3 2.3 ± 0.4 -0.6 ± 0.7
None B-C 0.0 ± 0.0 0.0 ± 0.0 4.7 ± 0.2 5.1 ± 0.5
None U-D 0.0 ± 0.0 0.0 ± 0.0 1.8 ± 0.0 -0.5 ± 0.1
None B-D 0.0 ± 0.0 0.0 ± 0.0 4.5 ± 0.2 3.7 ± 0.4
Chlorella vulgaris None 2.1 ± 0.7 0.0 ± 0.0 10.8 ± 0.6 22.1 ± 1.8
C. vulgaris and BESA None 1.3 ± 0.2 0.0 ± 0.0 12.0 ± 0.7 19.1 ± 5.1
Dunaliella tertiolecta None 2.8 ± 0.1 0.0 ± 0.0 3.0 ± 0.4 5.1 ± 0.1
D. tertiolecta and BESA None 1.5 ± 0.3 0.0 ± 0.0 4.2 ± 0.2 4.0 ± 0.3
C. vulgaris U-C 0.0 ± 0.0 74.9 ± 3.6 35.2 ± 0.3 -3.8 ± 1.0
C. vulgaris B-C 0.1 ± 0.0 0.0 ± 0.0 24.8 ± 0.0 31.2 ± 0.7
D. tertiolecta U-D 0.0 ± 0.0 4.7 ± 0.2 4.9 ± 0.0 0.4 ± 0.1
D. tertiolecta B-D 0.0 ± 0.0 0.0 ± 0.0 7.4 ± 0.8 8.9 ± 0.4
Glucose U-C 4.9 ± 0.4 56.4 ± 0.1 62.5 ± 0.4 2.9 ± 0.7
Glucose B-C 7.1 ± 0.4 0.0 ± 0.0 57.9 ± 0.9 46.1 ± 0.4
Glucose U-D 5.2 ± 0.9 38.5 ± 14.0 56.2 ± 7.0 13.3 ± 14.9
Glucose B-D 14.6 ± 2.3 0.0 ± 0.0 60.8 ± 0.5 44.4 ± 7.1
Cellulose U-C 0.0 ± 0.0 0.3 ± 0.4 0.9 ± 0.1 0.7 ± 1.4
Cellulose B-C 0.0 ± 0.0 0.0 ± 0.0 4.0 ± 0.0 7.6 ± 0.4
Cellulose U-D 0.0 ± 0.0 0.1 ± 0.1 1.8 ± 0.1 0.0 ± 0.1
Cellulose B-D 0.0 ± 0.0 0.0 ± 0.0 4.6 ± 0.3 5.4 ± 1.3
Chitosan U-C 0.0 ± 0.0 3.6 ± 4.1 2.4 ± 2.5 0.4 ± 1.5
Chitosan B-C 0.0 ± 0.0 0.0 ± 0.0 3.1 ± 0.3 6.9 ± 0.5
Chitosan U-D 0.0 ± 0.0 0.0 ± 0.0 1.0 ± 0.0 0.1 ± 0.1
Chitosan B-D 0.0 ± 0.0 0.0 ± 0.0 3.2 ± 0.1 5.6 ± 0.8
  1. A minus sign in front of sum of VFA and alcohols indicates that the sum of VFA and alcohols was higher on day 0 than on day 49. The values include standard errors.
  2. H2-fermenting cultures: C. vulgaris biomass as substrate = B-C; D. tertiolecta biomass as substrate = B-D; CH4-producing cultures: C. vulgaris biomass as substrate = U-C; D. tertiolecta biomass as substrate = U-D.
  3. BESA = 2-bromoethanesulfonic acid; VFA = volatile fatty acids.