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Table 1 Metabolite production in all cultures: cumulative gas production and accumulation of metabolites in the test cultures after 49 day of incubation

From: Biogenic hydrogen and methane production from Chlorella vulgaris and Dunaliella tertiolectabiomass

Substrate

Inoculum

H2(ml)

CH4(ml)

CO2(ml)

Sum of VFA and alcohols (mM)

None

U-C

0.0 ± 0.0

0.2 ± 0.3

2.3 ± 0.4

-0.6 ± 0.7

None

B-C

0.0 ± 0.0

0.0 ± 0.0

4.7 ± 0.2

5.1 ± 0.5

None

U-D

0.0 ± 0.0

0.0 ± 0.0

1.8 ± 0.0

-0.5 ± 0.1

None

B-D

0.0 ± 0.0

0.0 ± 0.0

4.5 ± 0.2

3.7 ± 0.4

Chlorella vulgaris

None

2.1 ± 0.7

0.0 ± 0.0

10.8 ± 0.6

22.1 ± 1.8

C. vulgaris and BESA

None

1.3 ± 0.2

0.0 ± 0.0

12.0 ± 0.7

19.1 ± 5.1

Dunaliella tertiolecta

None

2.8 ± 0.1

0.0 ± 0.0

3.0 ± 0.4

5.1 ± 0.1

D. tertiolecta and BESA

None

1.5 ± 0.3

0.0 ± 0.0

4.2 ± 0.2

4.0 ± 0.3

C. vulgaris

U-C

0.0 ± 0.0

74.9 ± 3.6

35.2 ± 0.3

-3.8 ± 1.0

C. vulgaris

B-C

0.1 ± 0.0

0.0 ± 0.0

24.8 ± 0.0

31.2 ± 0.7

D. tertiolecta

U-D

0.0 ± 0.0

4.7 ± 0.2

4.9 ± 0.0

0.4 ± 0.1

D. tertiolecta

B-D

0.0 ± 0.0

0.0 ± 0.0

7.4 ± 0.8

8.9 ± 0.4

Glucose

U-C

4.9 ± 0.4

56.4 ± 0.1

62.5 ± 0.4

2.9 ± 0.7

Glucose

B-C

7.1 ± 0.4

0.0 ± 0.0

57.9 ± 0.9

46.1 ± 0.4

Glucose

U-D

5.2 ± 0.9

38.5 ± 14.0

56.2 ± 7.0

13.3 ± 14.9

Glucose

B-D

14.6 ± 2.3

0.0 ± 0.0

60.8 ± 0.5

44.4 ± 7.1

Cellulose

U-C

0.0 ± 0.0

0.3 ± 0.4

0.9 ± 0.1

0.7 ± 1.4

Cellulose

B-C

0.0 ± 0.0

0.0 ± 0.0

4.0 ± 0.0

7.6 ± 0.4

Cellulose

U-D

0.0 ± 0.0

0.1 ± 0.1

1.8 ± 0.1

0.0 ± 0.1

Cellulose

B-D

0.0 ± 0.0

0.0 ± 0.0

4.6 ± 0.3

5.4 ± 1.3

Chitosan

U-C

0.0 ± 0.0

3.6 ± 4.1

2.4 ± 2.5

0.4 ± 1.5

Chitosan

B-C

0.0 ± 0.0

0.0 ± 0.0

3.1 ± 0.3

6.9 ± 0.5

Chitosan

U-D

0.0 ± 0.0

0.0 ± 0.0

1.0 ± 0.0

0.1 ± 0.1

Chitosan

B-D

0.0 ± 0.0

0.0 ± 0.0

3.2 ± 0.1

5.6 ± 0.8

  1. A minus sign in front of sum of VFA and alcohols indicates that the sum of VFA and alcohols was higher on day 0 than on day 49. The values include standard errors.
  2. H2-fermenting cultures: C. vulgaris biomass as substrate = B-C; D. tertiolecta biomass as substrate = B-D; CH4-producing cultures: C. vulgaris biomass as substrate = U-C; D. tertiolecta biomass as substrate = U-D.
  3. BESA = 2-bromoethanesulfonic acid; VFA = volatile fatty acids.