The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: is it an additive or synergistic effect?

Table 2 Effect of cellulase supplementation with xylanase and cellulase replacement with xylanase on cellulose and xylan hydrolysis, and on the degree of synergism during hydrolysis of SPCS after 72 h

Hydrolysis strategy	Total protein (mg/g cellulose)	Enzyme mixture	Xylanase (% total protein preparation)	DS	Cellulose hydrolysis (%)	Xylan hydrolysis (%)
Xylanase supplementation	40	35 mg C + 5 mg X	13	0.99	73.9	80.1
	45	35 mg C + 10 mg X	22	0.96	74.1	83.6
	45	35 mg C + 10 mg BSA	0	n/a	72.9	81.3
	95	35 mg C + 60 mg X	63	1.02	87.1	100
Cellulase replacement	35	35 mg C	0	n/a	72.1	81.6
		25 mg C + 10 mg X	29	0.98	74.1	82.6
		15 mg C + 20 mg X	57	1.09	74.2	82.6
		10 mg C + 25 mg X	71	1.31	82.3	98.6
		5 mg C + 30 mg X	86	1.62	86.3	99.3
		5 mg C + 30 mg BSA	0	n/a	55.8	68.6
Separate hydrolysis	5	5 mg C	0	n/a	45.6	56.3
	30	30 mg X	100	n/a	5.2	61.4

Separate hydrolysis used as control. BSA: bovine serum albumin; C: cellulases; DS: degree of synergism; SPCS: steam pretreated corn stover; X: xylanase; n/a: not applicable

ISSN: 2731-3654