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Table 2 Effect of cellulase supplementation with xylanase and cellulase replacement with xylanase on cellulose and xylan hydrolysis, and on the degree of synergism during hydrolysis of SPCS after 72 h

From: The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: is it an additive or synergistic effect?

Hydrolysis strategy Total protein (mg/g cellulose) Enzyme mixture Xylanase (% total protein preparation) DS Cellulose hydrolysis (%) Xylan hydrolysis (%)
Xylanase supplementation 40 35 mg C + 5 mg X 13 0.99 73.9 80.1
  45 35 mg C + 10 mg X 22 0.96 74.1 83.6
  45 35 mg C + 10 mg BSA 0 n/a 72.9 81.3
  95 35 mg C + 60 mg X 63 1.02 87.1 100
Cellulase replacement 35 35 mg C 0 n/a 72.1 81.6
   25 mg C + 10 mg X 29 0.98 74.1 82.6
   15 mg C + 20 mg X 57 1.09 74.2 82.6
   10 mg C + 25 mg X 71 1.31 82.3 98.6
   5 mg C + 30 mg X 86 1.62 86.3 99.3
   5 mg C + 30 mg BSA 0 n/a 55.8 68.6
Separate hydrolysis 5 5 mg C 0 n/a 45.6 56.3
  30 30 mg X 100 n/a 5.2 61.4
  1. Separate hydrolysis used as control. BSA: bovine serum albumin; C: cellulases; DS: degree of synergism; SPCS: steam pretreated corn stover; X: xylanase; n/a: not applicable