Table 2 S. cerevisiae strains and plasmids used in this study

EBY.VW4000

MATa leu2-3,112ura3-52 trp1-289 his3-Δ1 MAL2-8c SUC2 Δhxt1-17Δgal2 Δstl1 Δagt1 Δmph2 Δmph3

[26]

MKY06

MATa leu2-3,112ura3-52 trp1-289 his3-Δ1 MAL2-8c SUC2 Δhxt1-17Δgal2 Δstl1 Δagt1 Δmph2 Δmph3 promTAL1::loxP-prom-vkHXT7

This work

Plasmids

pUG6-kpHXT7

DNA-template for amplification of kanMX gene

with shortened HXT7 promoter for promoter substitution

[41]

pSH47

Cre-recombinase under control of GAL1 promoter, URA3 marker gene

[40]

pTHStp2

2μ plasmid expressed with the A. thaliana STP2 under control of shortened HXT7 promoter, URA3 marker gene

[8]

p423H7-synthIso

Codon-optimized Bacillus licheniformis araA in p423H7-6HIS

[4]

p424H7-synthKin

Codon-optimized E. coli araB in p424H7-6HIS, mutation in araB

[4]

p425H7-synthEpi

Codon-optimized E. coli araD in p425H7-6HIS

[4]

p426H7-6HIS

2μ plasmid, URA3 marker gene

[8]

pHL125^re

2μ plasmid with the GAL2 gene expressed under control of ADH1 promoter, URA3 marker gene, re-isolated

[47, 27]

p426-opt-AraT-S

2μ plasmid with the codon-optimized S. stipitis ARAT under control of shortened HXT7 promoter, URA3 marker gene

This work

p426-Stp2-HA

2μ plasmid expressed with a c-terminal, HA-tagged, full length version of the A. thaliana STP2 under control of shortened HXT7 promoter, URA3 marker gene

This work

pTHHXT9

2μ plasmid with the HXT9 gene expressed under control of shortened HXT7 promoter, URA3 marker gene

[8]

pTHHXT10

2μ plasmid with the HXT10 gene expressed under control of shortened HXT7 promoter, URA3 marker gene

[8]