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Table 2 S. cerevisiae strains and plasmids used in this study

From: Improving L-arabinose utilization of pentose fermenting Saccharomyces cerevisiaecells by heterologous expression of L-arabinose transporting sugar transporters

S. cerevisiae strain or plasmid Relevant genotype Source or reference
Strains   
EBY.VW4000 MATa leu2-3,112ura3-52 trp1-289 his3-Δ1 MAL2-8c SUC2 Δhxt1-17Δgal2 Δstl1 Δagt1 Δmph2 Δmph3 [26]
MKY06 MATa leu2-3,112ura3-52 trp1-289 his3-Δ1 MAL2-8c SUC2 Δhxt1-17Δgal2 Δstl1 Δagt1 Δmph2 Δmph3 promTAL1::loxP-prom-vkHXT7 This work
Plasmids   
pUG6-kpHXT7 DNA-template for amplification of kanMX gene
with shortened HXT7 promoter for promoter substitution
[41]
pSH47 Cre-recombinase under control of GAL1 promoter, URA3 marker gene [40]
pTHStp2 2μ plasmid expressed with the A. thaliana STP2 under control of shortened HXT7 promoter, URA3 marker gene [8]
p423H7-synthIso Codon-optimized Bacillus licheniformis araA in p423H7-6HIS [4]
p424H7-synthKin Codon-optimized E. coli araB in p424H7-6HIS, mutation in araB [4]
p425H7-synthEpi Codon-optimized E. coli araD in p425H7-6HIS [4]
p426H7-6HIS 2μ plasmid, URA3 marker gene [8]
pHL125re 2μ plasmid with the GAL2 gene expressed under control of ADH1 promoter, URA3 marker gene, re-isolated [47, 27]
p426-opt-AraT-S 2μ plasmid with the codon-optimized S. stipitis ARAT under control of shortened HXT7 promoter, URA3 marker gene This work
p426-Stp2-HA 2μ plasmid expressed with a c-terminal, HA-tagged, full length version of the A. thaliana STP2 under control of shortened HXT7 promoter, URA3 marker gene This work
pTHHXT9 2μ plasmid with the HXT9 gene expressed under control of shortened HXT7 promoter, URA3 marker gene [8]
pTHHXT10 2μ plasmid with the HXT10 gene expressed under control of shortened HXT7 promoter, URA3 marker gene [8]
  1. HA; hemagglutinin.
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