Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse

Table 6 Gene expression measured by real-time RT-PCR of genes encoding Aspergillus niger putative transporters in presence of xylose or xylose and glucose

Gene locus	Control (fructose)	Xylose 1 hour	Xylose 4 hours	Xylose + glucose 1 hour	Xylose + glucose 4 hours
An06g00620	0.08 ± 0.03	0.42 ± 0.07	0.38 ± 0.05	0.003 ± 0.00	0.03 ± 0.01
An15g04270	0.05 ± 0.00	0.04 ± 0.00	0.20 ± 0.00	0.04 ± 0.00	0.02 ± 0.00
An15g05440	0.34 ± 0.04	0.24 ± 0.03	0.56 ± 0.12	0.09 ± 0.05	0.19 ± 0.05
An11g03700	0.06 ± 0.00	0.04 ± 0.01	0.17 ± 0.01	0.009 ± 0.001	0.05 ± 0.01
An12g09270	0.29 ± 0.04	0.20 ± 0.02	0.56 ± 0.03	0.010 ± 0.001	0.20 ± 0.05
An01g00850	0.25 ± 0.01	0.16 ± 0.03	0.60 ± 0.02	0.13 ± 0.01	0.45 ± 0.17
An06g00260	0.70 ± 0.03	2.40 ± 0.22	2.30 ± 0.64	0.25 ± 0.01	0.36 ± 0.07

The mRNA accumulation of several A. niger transporter-encoding genes during growth on minimal medium (MM) + xylose or MM + xylose + glucose. Cultures from the A. niger N402 strain were grown for 24 hours at 30°C in batch cultivation medium (BCM) + 50 mM fructose and the mycelia were transferred to either BCM without yeast extract + 1% xylose or BCM without yeast extract + 1% xylose + 2% glucose and grown for one and four hours at 30°C. Real-time RT-PCR was used to quantify mRNA. The measured quantity of a specific gene mRNA in each of the treated samples was normalized using the comparative threshold (C_t) values obtained for the actin mRNA amplifications run in the same plate. The relative quantitation of a specific gene and actin gene expression was determined by drawing a standard curve (that is, C_t values plotted against logarithm of DNA copy numbers). The results of four sets of experiments were combined for each determination. Data presented are means ± SD. The values represent the cDNA concentration of a specific gene divided by the actin cDNA concentration.

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