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Table 6 Gene expression measured by real-time RT-PCR of genes encoding Aspergillus niger putative transporters in presence of xylose or xylose and glucose

From: Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse

Gene locus Control (fructose) Xylose 1 hour Xylose 4 hours Xylose + glucose 1 hour Xylose + glucose 4 hours
An06g00620 0.08 ± 0.03 0.42 ± 0.07 0.38 ± 0.05 0.003 ± 0.00 0.03 ± 0.01
An15g04270 0.05 ± 0.00 0.04 ± 0.00 0.20 ± 0.00 0.04 ± 0.00 0.02 ± 0.00
An15g05440 0.34 ± 0.04 0.24 ± 0.03 0.56 ± 0.12 0.09 ± 0.05 0.19 ± 0.05
An11g03700 0.06 ± 0.00 0.04 ± 0.01 0.17 ± 0.01 0.009 ± 0.001 0.05 ± 0.01
An12g09270 0.29 ± 0.04 0.20 ± 0.02 0.56 ± 0.03 0.010 ± 0.001 0.20 ± 0.05
An01g00850 0.25 ± 0.01 0.16 ± 0.03 0.60 ± 0.02 0.13 ± 0.01 0.45 ± 0.17
An06g00260 0.70 ± 0.03 2.40 ± 0.22 2.30 ± 0.64 0.25 ± 0.01 0.36 ± 0.07
  1. The mRNA accumulation of several A. niger transporter-encoding genes during growth on minimal medium (MM) + xylose or MM + xylose + glucose. Cultures from the A. niger N402 strain were grown for 24 hours at 30°C in batch cultivation medium (BCM) + 50 mM fructose and the mycelia were transferred to either BCM without yeast extract + 1% xylose or BCM without yeast extract + 1% xylose + 2% glucose and grown for one and four hours at 30°C. Real-time RT-PCR was used to quantify mRNA. The measured quantity of a specific gene mRNA in each of the treated samples was normalized using the comparative threshold (Ct) values obtained for the actin mRNA amplifications run in the same plate. The relative quantitation of a specific gene and actin gene expression was determined by drawing a standard curve (that is, Ct values plotted against logarithm of DNA copy numbers). The results of four sets of experiments were combined for each determination. Data presented are means ± SD. The values represent the cDNA concentration of a specific gene divided by the actin cDNA concentration.