Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse

Table 7 Protein identification by mass spectrometry from Aspergillus niger supernatants grown for 24 hours on steam-exploded sugarcane bagasse

Sample label	Protein name	Gene locus	NCBI GEO accession number	Theoretical molecular mass (kDa)	Score	Sequence coverage (%)	GE level (log₂ Cy5/Cy3)
1	β-glucosidase	An18g03570	[GEO:215260053]	90	107	16	2.56 ± 0.03
1	Xylosidase (XlnD)	An01g09960	[GEO:145230215]	87	176	18	2.10 ± 0.05
2	Glucan 1,4-α-glucosidase (GlaA)	An03g06550	[GEO:145235763]	68	264	40	ND
2	Hypothetical protein	An14g02470	[GEO:145249068]	65	63	15	ND
3	1,4-β-D-glucan cellobiohydrolase B (CbhB)	An01g11660	[GEO:145235763]	56	264	40	6.59 ± 0.15
3	Acid α-amylase	An11g03340	[GEO:145243632]	55	63	13	ND
3	α-N-arabinofuranosidase B	An15g02300	[GEO:1168267]	52	256	23	7.94 ± 0.10
4	1,4-β-D-glucan cellobiohydrolase A	An07g09330	[GEO:74698499]	48	158	12	3.89 ± 0.20
4	Candidate cellulose 1,4-β-cellobiosidase II (Cbh2)	An12g02220	[GEO:145246118]	48	89	18	4.37 ± 0.05
5	Aspergillopepsin A	An14g04710	[GEO:1709632]	41	126	23	5.83 ± 0.09
6	Endo-1,4-β-xylanase A precursor	An03g00940	[GEO:145234695]	35	219	67	3.64 ± 0.06
6	1,4-β-D-arabinoxylan arabinofuranohydrolase (AxhA)	An03g00960	[GEO:145234699]	35	127	37	4.03 ± 0.01
6	Endo-1,4-β-xylanase C	An03g00940	[GEO:292495635]	35	230	57	3.64 ± 0.06
6	Protease B	An14g04710	[GEO:1585070]	34	136	38	5.83 ± 0.09
7	Chain A, crystal structure of ferulic acid esterase	An09g00120	[GEO:48425840]	28	105	40	6.17 ± 0.99
8	Endoglucanase A	An14g02760	[GEO:289595328]	25	99	22	4.36 ± 0.30
9	Xylanase	An01g00780	[GEO:13242071]	11	99	78	8.29 ± 0.04

NCBI GEO = National Center for Biotechnology Information Gene Expression Omnibus; kDa = kilodaltons; GE = gene expression; log₂ Cy5/Cy3 ratio = base 2 logarithm ratio of cyanine 5 to cyanine 3; ND = no data. Data presented in right-hand column are means ± SD. Score column refers to Mowse scoring algorithm [45].

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