Figure 7From: A strain of Saccharomyces cerevisiaeevolved for fermentation of lignocellulosic biomass displays improved growth and fermentative ability in high solids concentrations and in the presence of inhibitory compoundsVerification of phenotype in individually isolated clones from the evolved yeast population. Glycerol freezer stock samples from AJP50 fermentations plated onto yeast-peptone-dextrose (YPD) agar containing all 13 inhibitory compounds (YPD-AI) yielded isolated colonies. Individual colonies were then subcultured on additional YPD-AI once (two platings), twice (three platings), or thrice (four platings), as described in Methods. Isolated colonies were grown in YPD broth containing all 13 inhibitory compounds (YPD-BI). Growth curves for each series of platings was plotted as a single line with error bars representing one standard deviation from the mean (two platings (red), n = 20; three platings (blue), n = 20; four platings (green), n = 40). XR122N did not grow in YPD-BI or YPD-AI, and is not represented on the graph.Back to article page