Verification of phenotype in individually isolated clones from the evolved yeast population. Glycerol freezer stock samples from AJP50 fermentations plated onto yeast-peptone-dextrose (YPD) agar containing all 13 inhibitory compounds (YPD-AI) yielded isolated colonies. Individual colonies were then subcultured on additional YPD-AI once (two platings), twice (three platings), or thrice (four platings), as described in Methods. Isolated colonies were grown in YPD broth containing all 13 inhibitory compounds (YPD-BI). Growth curves for each series of platings was plotted as a single line with error bars representing one standard deviation from the mean (two platings (red), n = 20; three platings (blue), n = 20; four platings (green), n = 40). XR122N did not grow in YPD-BI or YPD-AI, and is not represented on the graph.