Table 1 Amino acid sequences and glycosyl hydrolase families for all six bacterial enzymes.

LX3

MSGNALRDYAEARGIKIGTCVNYPFYNNSDPTYNSILQREFSMVVCENEMKFDALQPRQNVFDFSKGDQLLAFAERNGMQMRGHTLIWHNQNPSWLTNGNWNRDSLLAVMKNHITTVMTHYKGKIVEWDVANECMDDSGNGLRSSIWRNVIGQDYLDYAFRYAREADPDALLFYNDYNIEDLGPKSNAVFNMIKSMKERGVPIDGVGFQCHFINGMSPEYLASIDQNIKRYAEIGVIVSFTEIDIRIPQSENPATAFQVQANNYKELMKICLANPNCNTFVMWGFTDKYTWIPGTFPGYGNPLIYDSNYNPKPAYNAIKEALMGYHHHHHH

10

P10478

Truncated Clostridium thermocellum

(XynZ, 38.0 kDa)

(corresponds to last 324 A.A. in protein of the mature enzyme)

LX4

MKMGKMYEVALVVEGYQSSGKADVTSMTITVGNAPSTSSPPGPTPEPTPRSAFSKIEAEEYNSLKSSTIQTIGTSDGGSGIGYIESGDYLVFNKINFGNGANSFKARVASGADTPTNIQLRLGSPTGTLIGTLTVASTGGWNNYEEKSCSITNTTGQHDLYLVFSGPVNIDYFIFDSNGVNPTPTSQPQQGQVLGDLNGDKQVNSTDYTALKRHLLNITRLSGTALANADLNGDGKVDSTDLMILHRYLLGIISSFPRSNPQPSSNPQPSSNPQPTINPNAKLVALTFDDGPDNVLTARVLDKLDKYNVKATFMVVGQRVNDSTAAIIRRMVNSGHEIGNHSWSYSGMANMSPDQIRKSIADTNAVIQKYAGTTPKFFRPPNLETSPTLFNNVDLVFVGGLTANDWIPSTTAEQRAAAVINGVRDGTIILLHDVQPEPHPTPEALDIIIPTLKSRGYEFVTLTELFTLKGVPIDPSVKRMYNSVP

11

O87119

Truncated C. thermocellum

(XynA, 52.1 kDa)

(corresponds to residues 199-683 of the mature enzyme)

LX5

KNKRVLAKITALVVLLGVFFVLPSNISQLYADYEVVHDTFEVNFDGWCNLGVDTYLTAVENEGNNGTRGMMVINRSSASDGAYSEKGFYLDGGVEYKYSVFVKHNGTGTETFKLSVSYLDSETEEENKEVIATKDVVAGEWTEISAKYKAPKTAVNITLSITTDSTVDFIFDDVTITRKGMAEANTVYAANAVLKDMYANYFRVGSVLNSGTVNNSSIKALILREFNSITCENEMKPDATLVQSGSTNTNIRVSLNRAASILNFCAQNNIAVRGHTLVWHSQTPQWFFKDNFQDNGNWVSQSVMDQRLESYIKNMFAEIQRQYPSLNLYAYDVVNEAVSDDANRTRYYGGAREPGYGNGRSPWVQIYGDNKFIEKAFTYARKYAPANCKL

YYNDYNEYWDHKRDCIASICANLYNKGLLDGVGMQSHINADMNGFSGIQNYKAALQKYINIGCDVQITELDISTENGKFSLQQQADKYKAVFQAAVDINRTSSKGKVTAVCVWGPNDANTWLGSQNAPLLFNANNQPKPAYNAVASIIPQSEWGDGNNPAGGGGGGKPEEPDANGYYYHDTFEGSVGQWTARGPAEVLLSGRTAYKGSESLLVRNRTAAWNGAQRALNPRTFVPGNTYCFSVVASFIEGASSTTFCMKLQYVDGSGTQRYDTIDMKTVGPNQWVHLYNPQYRIPSDATDMYVYVETADDTINFYIDEAIGAVAGTVIEGPAPQPTQPPVLLGDVNG

10

B4BME8

Truncated C. thermocellum

(XynY, 81.4 kDa)

(corresponds to residues 1-736 of mature enzyme)

LX6

MEIPSLKEVYKDYFPIGAAVSHLNIYTYEDLLKKHFNSLTPENQMKWEVIHPKPYVYDFGPADEIVDFAMKNGMKVRGHTLVWHNQTPGWVYAGTKDEILARLKEHIYEVVGHYKGKVYAWDVVNEALSDNPNEFLRKAPWYDICGEEVIEKAFIWANEADPNAKLFYNDYNLEDPIKREKAYQLVKRLKEKGIPIHGVGIQGHWTLAWPTPKMLEDSIKRFSELGVEVQITEFDISIYYDRNENNNFKVPPDDRIEKQAQLYKQAFEILRKYRGVVTGVTFWGVADDYTWLYFWPVRGREDYPLLFDKNHNPKKAFWEIVKFHHHHHH

10

B8E346

Dictyoglomus turgidum

(Dtur_1647, 38.1 kDa)

LαGl

MAEVKPYNMCWLEYTDLSKYKNKYIKVFENVVVLGGNELNLPLKELKNFLTFSLNIKPKIFKNTLVKGRNYVLIGRLIEIKKIFKESERFEKLLNDEGFIIKRIDIDGNKVLIITAKSYNGIVYGIFNLIERLKRGEDIENIDIVSNPSLRFRMLNHWDNLDGSIERGYAGKSIFFRENKILINERTKDYARLLSSIGVNGVVINNVNVKKKEVELITPSYLKKIGELSKIFSAYGIKIY

LSINFASPIYLGGLNTADPLDKRVAVWWKAKVDEIYEYVPDFGGFLVKADSEFNPGPHMYGRTHADGANMLGEALESYGGFVIWRAFVYNCLQDWRDTNTDRAKAAYENFKPLDGKFSENVIVQIKYGPMDFQVREPVNPLFGGLEHTNQILELQITQEYTGQQIHLCYLGTLWKEVLDFDTYAKGEGSKVKEILKGNVFDLKNNGMAGVSNVGDDINWTGHDLAQANLYTFGALSWNPDERIEEVVKRWIELTFGDNEKVIKNISYMLLSSHKAYEKYTTPLGLGWMVNPGHHYGPNPEGYEYSKWGTYHRANYEAIGVDRSSRGTGYTLQYHSPWREIYDNIETCPEELLLFFHRVPYNYKLKSGKTLIQTYYDLHFEGVEEAEEIRKKWIELKGEIEDKIYERVLNRLDIQIEHAKEWRDVINTYFFRRTGIPDEKGRKIYPHHHHHH

67

B8E3B2

D. turgidum

(Dtur_1714, 79.4 kDa)

LArb

MSVKRAQMIIEKDFKIAKIDKRIYGSFIEHLGRAVYGGIYEPGHPQADENGFRQDVIEMVKELQVPIIRYPGGNFVSGYNWEDGVGPKEKRPRRLDLAWKSVETNEIGLNEFVDWAKMVGAEVNMAVNLGTRGIDAARNLVEYCNHPSGSYYSDLRISHGYKEPHKIKTWCLGNEMDGPWQIGHKTAVEYGRIACEAAKVMKWVDPTIELVACGSSGRNMPTFAEWEAIVLDHTYEHVDYISLHQYFGNRDNDTANYLAMSLEMDDFIRSVVAIADYVKAKKRSKKTIHLSFDEWNVWYHSNEADKQIEPWTVAPPLLEDIYNFEDALLVGCMLITLMKHADRVKIACLAQLVNVIAPIM

TEKNGPAWKQTIYYPFMHASVYGRGVALHPVISSPKYDSKDFTDVPYLESIAVYNEEKEEVTIFAVNRDMEDSLLLECDVRNFEDYRVIEHIVLEHENVKQTNSAQSSPVVPHRNGNAQM

51

B4BMB8

Geobacillus sp. G11MC16

(57.1 kDa)