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Table 2 Activities against different substrates (including CSCB) and protein content in termites.

From: High throughput screening of hydrolytic enzymes from termites using a natural substrate derived from sugarcane bagasse

  Activities (mU/animal); specific activities in parentheses (mU/mg protein)  
Species Laminarin CMC Pectin Xylan Cellulose pNPβGlu pNPβXyl CSCB Protein (mg/animal)
H. tenuis 0.20 ± 0.02 0.070 ± 0.004 0.11 ± 0.01 0.28 ± 0.04 1.3 ± 0.1 2.4 ± 0.1 0.36 ± 0.02 0.19 ± 0.02 0.10 ± 0.01 a,b
  (2.2 ± 0.2) a,b (0.77 ± 0.07) a (1.3 ± 0.2) a (3.0 ± 0.4) a (14 ± 2) a (40 ± 2) d (6.2 ± 0.3) a (2.2 ± 0.3) a  
C. gestroi 0.49 ± 0.05 0.14 ± 0.02 0.28 ± 0.07 0.55 ± 0.09 1.6 ± 0.2 7.3 ± 0.3 0.84 ± 0.08 0.43 ± 0.02 0.09 ± 0.01 a,b
  (6.2 ± 0.6) a,b,c (1.6 ± 0.3) a (3.6 ± 0.9) a (7 ± 1) a,b (17 ± 2) a (94 ± 3) f (11 ± 1) a (4.7 ± 0.8) a  
Grigiotermes sp. 2.7 ± 0.2 0.32 ± 0.04 0.27 ± 0.04 0.15 ± 0.04 0.022 ± 0.008 2.3 ± 0.1 2.29 ± 0.08 0.07 ± 0.01 0.14 ± 0.02 c
  (19 ± 5) d,e (2.2 ± 0.8) a (1.8 ± 0.5) a (0.8 ± 0.3) a (0.20 ± 0.07) b (57 ± 5) e (58 ± 4) c (0.45 ± 0.05) a  
S. nigrostomus 2.0 ± 0.2 1.06 ± 0.06 1.0 ± 0.2 1.4 ± 0.2 0.17 ± 0.04 2.4 ± 0.2 0.12 ± 0.02 0.930 ± 0.009 0.09 ± 0.01 a,b
  (22 ± 1) e (11.7 ± 0.6) b (11 ± 2) b (16 ± 3) b (2.3 ± 0.5) b (33 ± 4) c,d (1.2 ± 0.5) a (13 ± 3) b  
M. strunckii 0.030 ± 0.001 0.10 ± 0.01 0.10 ± 0.01 0.19 ± 0.02 0.30 ± 0.05 1.5 ± 0.2 0.17 ± 0.01 0.11 ± 0.03 0.10 ± 0.01 a,b
  (0.42 ± 0.02) a (1.3 ± 0.1) a (1.23 ± 0.09) a (2.4 ± 0.3) a (2.7 ± 0.6) b (17 ± 2) b (1.9 ± 0.2) a (1.0 ± 0.4) a  
Orthognathoterme 4.5 ± 0.4 0.13 ± 0.02 0.6 ± 0.1 0.10 ± 0.02 0 0.17 ± 0.03 0.16 ± 0.03 0.020 ± 0.005 0.26 ± 0.03 d
s sp 2 (14 ± 1) c,d,e (0.42 ± 0.07) a (1.9 ± 0.4) a (0.32 ± 0.07) a (0) b (1.2 ± 0.2) a (1.1 ± 0.1) a (0.38 ± 0.09) a  
C. silvestrii 2.3 ± 0.3 0.53 ± 0.09 0.57 ± 0.06 1.61 ± 0.07 0.23 ± 0.03 1.39 ± 0.01 2.67 ± 0.03 0.10 ± 0.01 0.14 ± 0.01 b,c
  (19 ± 2) d,e (4.3 ± 0.8) a (4.6 ± 0.6) a (12.9 ± 0.7) b (1.5 ± 0.2) b (26 ± 1) b,c (44 ± 5) b (0.64 ± 0.04) a  
S. dirus 5.3 ± 0.4 0.9 ± 0.3 0.59 ± 0.04 0.26 ± 0.06 0.6 ± 0.2 9 ± 1 0.7 ± 0.1 0.16 ± 0.04 0.18 ± 0.01 c
  (9 ± 1) a,b,c,d (2.3 ± 0.4) a (1.0 ± 0.1) a (0.4 ± 0.1) a (4 ± 1) b (25 ± 4) b,c (2.3 ± 0.5) a (2.1 ± 0.7) a  
N. jaraguae 0.56 ± 0.07 1.5 ± 0.1 0.80 ± 0.04 2.1 ± 0.3 0.17 ± 0.04 3.2 ± 0.1 0.4 ± 0.1 0.15 ± 0.03 0.040 ± 0.003 a
  (8 ± 1) a,b,c (15 ± 1) b,c (20 ± 1) c (44 ± 7) c (3.8 ± 0.5) b (68 ± 4) e (9 ± 1) a (3.8 ± 1.1) a  
N. bivalens 0.89 ± 0.09 1.5 ± 0.3 1.5 ± 0.1 3.15 ± 0.05 0.38 ± 0.02 1.9 ± 0.2 0.62 ± 0.02 0.140 ± 0.005 0.10 ± 0.02 a,b
  (11 ± 1) b,c,d (20 ± 3) c (17 ± 2) c (37 ± 1) c (4 ± 1) b (32 ± 2) b,c,d (10.8 ± 0.2) a (1.5 ± 0.4) a  
  1. Figures are means ± standard error of the mean. See Materials and methods for details. Statistical analysis (ANOVA) was carried out with SPSS 8.0. Specific activities for each substrate were compared across species. Each column was submitted to one-way ANOVA and pairs of figures were compared using Tukey's honestly significant difference test, with significance level of 0.05. a,b,c,d In a column, groups with the same superscript letter are not significantly different. CMC: carboxymethylcellulose; CSCB: colloidal sugar cane bagasse; pnPβGlu: p-nitrophenyl-β-glucoside; pNPβXyl: p-nitrophenyl-β-xyloside.