Table 1 Plasmids and strains used in this study
Plasmids/strains | Relevant characteristics | Source |
|---|---|---|
Plasmids | Â | Â |
   pGEM-T Easy | PCR product cloning, ampR | Promega (Madison, WI, USA) |
   pRSETB | ampR, T7p-T7t | Invitrogen (Carlsbad, CA, USA) |
   p426TEF | URA3 TEFp-CYC1t | [43] |
   pRESTB-XIPiromyces | AmpR, T7p-xiPiromyces-T7t | This study |
   pRSETB-Xym1 | AmpR, T7p-xym1-T7t | This study |
   pRSETB-Xym2 | AmpR, T7p-xym2-T7t | This study |
   p426TEF-XiPiromyces | URA3, p426TEFp-XiPiromyces-CYC1t | This study |
   p426TEF-Xym1 | URA3, p426TEFp-xym1-CYC1t | This study |
   p426TEF-Xym2 | URA3, p426TEFp-xym2-CYC1t | This study |
Escherichia coli strains | Â | Â |
   ElectroTen-Blue | KanR, TetR | Stratagene (La Jolla, California, USA) |
   HB101 | ara-14, proA2, xyl-5, leuB6, thi-1 | Takara Bio (Otsu, Shiga, Japan) |
   DH5α | SmR | Life Technologies (Rockville, MD, USA) |
   TMB2010 | HB101 + pRSETB | This study |
   TMB2011 | HB101 + pRSETB-xiPiromyces | This study |
   TMB2012 | HB101 + pRSETB-Xym1 | This study |
   TMB2013 | HB101 + pRSETB-Xym2 | This study |
Saccharomyces cerevisiae strains | Â | Â |
   TMB3044 | CEN.PK 2-1C Δgre3, his3::PGK1 p-XKS1-PGK1 t,TAL1::PGK1 p-TAL1-PGK1 t,TKL1::PGK1 p-TKL1-PGK1 t, RKI1::PGK1 p-RKI1-PGK1 t, RPE1::PGK1 p-RPE1-PGK1 t,ura3 | [26] |
   TMB3363 | TMB3044, p426TEF | This study |
   TMB3359 | TMB3044, p426TEF-XiPiromyces | This study |
   TMB3364 | TMB3044, p426TEF-Xym1 | This study |
   TMB3365 | TMB3044, p426TEF-Xym2 | This study |