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Table 1 Plasmids and strains used in this study

From: Isolation of xylose isomerases by sequence- and function-based screening from a soil metagenomic library

Plasmids/strains Relevant characteristics Source
Plasmids   
   pGEM-T Easy PCR product cloning, ampR Promega (Madison, WI, USA)
   pRSETB ampR, T7p-T7t Invitrogen (Carlsbad, CA, USA)
   p426TEF URA3 TEFp-CYC1t [43]
   pRESTB-XIPiromyces AmpR, T7p-xiPiromyces-T7t This study
   pRSETB-Xym1 AmpR, T7p-xym1-T7t This study
   pRSETB-Xym2 AmpR, T7p-xym2-T7t This study
   p426TEF-XiPiromyces URA3, p426TEFp-XiPiromyces-CYC1t This study
   p426TEF-Xym1 URA3, p426TEFp-xym1-CYC1t This study
   p426TEF-Xym2 URA3, p426TEFp-xym2-CYC1t This study
Escherichia coli strains   
   ElectroTen-Blue KanR, TetR Stratagene (La Jolla, California, USA)
   HB101 ara-14, proA2, xyl-5, leuB6, thi-1 Takara Bio (Otsu, Shiga, Japan)
   DH5α SmR Life Technologies (Rockville, MD, USA)
   TMB2010 HB101 + pRSETB This study
   TMB2011 HB101 + pRSETB-xiPiromyces This study
   TMB2012 HB101 + pRSETB-Xym1 This study
   TMB2013 HB101 + pRSETB-Xym2 This study
Saccharomyces cerevisiae strains   
   TMB3044 CEN.PK 2-1C Δgre3, his3::PGK1 p-XKS1-PGK1 t,TAL1::PGK1 p-TAL1-PGK1 t,TKL1::PGK1 p-TKL1-PGK1 t, RKI1::PGK1 p-RKI1-PGK1 t, RPE1::PGK1 p-RPE1-PGK1 t,ura3 [26]
   TMB3363 TMB3044, p426TEF This study
   TMB3359 TMB3044, p426TEF-XiPiromyces This study
   TMB3364 TMB3044, p426TEF-Xym1 This study
   TMB3365 TMB3044, p426TEF-Xym2 This study