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Table 3 Effect of metal ions and reducing agents on the activity of purified NpaBGS

From: A highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5

Metal 1 mM 10 mM
AlCl3.6H2O 104 7
MgSO4.7H2O 132 152
CaCl2.2 H2O 109 136
MgCl2.6 H2O 132 150
MnCl2.2 H2O 142 151
ZnCl2 116 133
CuCl2.2 H2O 97 3
FeCl3.2 H2O 95 37
β-mercaptoenthanol 104 105
Dithiothreitol 103 101
EDTA 100 91
control (NpaBGS at 1:100) 100 100
  1. The assay was performed at 40°C, pH 6 for 1 hr using 0.01units/ml of the NpaBGS enzyme. The relative activities are expressed as percentage by normalizing to the control activity.