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Table 3 Effect of metal ions and reducing agents on the activity of purified NpaBGS

From: A highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5

Metal

1 mM

10 mM

AlCl3.6H2O

104

7

MgSO4.7H2O

132

152

CaCl2.2 H2O

109

136

MgCl2.6 H2O

132

150

MnCl2.2 H2O

142

151

ZnCl2

116

133

CuCl2.2 H2O

97

3

FeCl3.2 H2O

95

37

β-mercaptoenthanol

104

105

Dithiothreitol

103

101

EDTA

100

91

control (NpaBGS at 1:100)

100

100

  1. The assay was performed at 40°C, pH 6 for 1 hr using 0.01units/ml of the NpaBGS enzyme. The relative activities are expressed as percentage by normalizing to the control activity.