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Table 2 Number of transformants isolated when Dcm methylation site in pyrF is removed by silent mutation

From: Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum

   Average number of transformants per µg DNA (range)
E. colihosta Methylationb pAMG205 pAMG206 pAMG205Δdcm#7
Top10 Dam+, dcm+ 2 (0−6) 160 (95−240) 220 (60−520)
BL21 Dam+, dcm- 1200 (680−1700) 1400 (1100−1900) 1500 (840−2200)
  1. aE. coli strain from which plasmid DNA was isolated.
  2. bState of plasmid methylation by E. coli Dam and Dcm DNA methylases.