Table 2 Number of transformants isolated when Dcm methylation site in pyrF is removed by silent mutation
From: Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum
|  |  | Average number of transformants per µg DNA (range) | ||
|---|---|---|---|---|
E. colihosta | Methylationb | pAMG205 | pAMG206 | pAMG205Δdcm#7 |
Top10 | Dam+, dcm+ | 2 (0−6) | 160 (95−240) | 220 (60−520) |
BL21 | Dam+, dcm- | 1200 (680−1700) | 1400 (1100−1900) | 1500 (840−2200) |