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Table 4 Strains and plasmids

From: Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum

Strain or plasmid Relevant features Source/reference
Microbial strains   
S. cerevisiae InvSc1 uracil auxotroph Invitrogen
E. coli Top10 dam+dcm+ Invitrogen
E. coli C2925 dam- dcm- New England Biolabs
E. coli BL21 (DE3) dam+dcm- New England Biolabs
Plasmids   
pAMG205 oriColE1, bla, CEN6, ARSH4, URA3, PgapD-cat-pyrF, pNW33N replication origin This study
pAMG206 oriColE1, bla, CEN6, ARSH4, URA3, PgapD-cat-hpt, pNW33N replication origin This study
pAMG205dcm7 pAMG205pyrF::pyrF* This study
pMU1054 oriColE1, bla, P-gapD-cat, pB6A origin This study
pMU1117 oriColE1, bla, P-gapD-cat, pMK3 origin This study