Table 4 Strains and plasmids
From: Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum
Strain or plasmid | Relevant features | Source/reference |
|---|---|---|
Microbial strains | ||
S. cerevisiae InvSc1 | uracil auxotroph | Invitrogen |
E. coli Top10 | dam+dcm+ | Invitrogen |
E. coli C2925 | dam- dcm- | New England Biolabs |
E. coli BL21 (DE3) | dam+dcm- | New England Biolabs |
Plasmids | ||
pAMG205 | oriColE1, bla, CEN6, ARSH4, URA3, PgapD-cat-pyrF, pNW33N replication origin | This study |
pAMG206 | oriColE1, bla, CEN6, ARSH4, URA3, PgapD-cat-hpt, pNW33N replication origin | This study |
pAMG205â–³dcm7 | pAMG205â–³pyrF::pyrF* | This study |
pMU1054 | oriColE1, bla, P-gapD-cat, pB6A origin | This study |
pMU1117 | oriColE1, bla, P-gapD-cat, pMK3 origin | This study |